The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae

European Journal of Cell Biology

Volumn 70, Issue 4, 1996, Pages 289-297

  Chen, Yen Ju ^a   Stevens, Tom H ^a  

^a UNIVERSITY OF OREGON   (United States)

Author keywords

Golgi retention; Membrane traffic; Receptor localization; Vacuolar protein sorting; Yeast vacuole

Indexed keywords

ALKALINE PHOSPHATASE; CELL ENZYME; CELL RECEPTOR; GENE PRODUCT; HYDROLASE; MEMBRANE PROTEIN; PROTEINASE; SERINE CARBOXYPEPTIDASE;

ARTICLE; CELL MUTANT; CELL STRUCTURE; CELL VACUOLE; CONTROLLED STUDY; DNA DETERMINATION; ENZYME RELEASE; GENE FUNCTION; GOLGI COMPLEX; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TARGETING; SACCHAROMYCES CEREVISIAE; YEAST CELL;

EID: 0029816236     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, D. J. Lipman: Basic local alignment search tool. J. Mol. Biol. 215, 403-410 (1990).
2. Bankaitis, V. A., L. M. Johnson, S. D. Emr: Isolation of yeast mutants defective in protein targeting to the vacuole. Proc. Natl. Acad. Sci. USA 83, 9075-9079 (1986).
3. Banta, L. M., J. S. Robinson, D. J. Klionsky, S. D. Emr: Organelle assembly in yeast: characterization of yeast mutants efective in vacuolar biogenesis and protein sorting. J. Cell Biol. 107, 1369-1383 (1988).
4. Boeke, J. D., F. LaCroute, G. R. Fink: A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast:5-fluororotic acid resistance. Mol. Gen. Genet. 197, 345-346 (1984).
5. Carlson, M., D. Botstein: Two differentially regulated mRNAs with different 5′ ends encode secreted proteins with intracellular forms of yeast invertase. Cell 28, 145-154 (1982).
6. Casadaban, M. F., S. N. Cohen: Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138, 179-207 (1980).
7. Cereghino, J. L., E. G. Marcusson, S. D. Emr: The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization. Mol. Biol. Cell 6, 1089-1102 (1995).
8. Cooper, A., H. Bussey: Yeast Kex1p is a Golgi-associated membrane protein: deletions in a cytoplasmic targeting domain result in mislocalization to the vacuolar membrane. J. Cell Biol. 119, 1459-1468 (1992).
9. Cooper, A. A., T. H. Stevens: Vps10p cycles between the late Golgi and the prevacuolar compartment in its function as the sorting receptor for multiple yeast vacuolar hydrolases. J. Cell Biol. 133, in press (1996).
10. Graham, T. R., S. D. Emr: Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant. J. Cell Biol. 114, 207-218 (1991).
11. Hill, J. E., A. M. Myers, T. J. Koerner, A. Tzagoloff: E. coli shuttle vectors with multiple unique restriction sites. Yeast 2, 163-167 (1986).
12. Hill, K. J., T. H. Stevens: Vma22p is a novel endoplasmic reticulum-associated protein required for assembly of the yeast vacuolar H+-ATPase complex. J. Biol. Cliem. 270, 22329-22336 (1995).
13. Horazdovsky, B. F, S. D. Emr: The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast. J. Biol. Chem. 268, 4953-4962 (1993).
14. Ito, H., Y. Fukuda, K. Murata, A. Kimura: Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168 (1983).
15. Jones, E. W.: Proteinase mutants of Saccharomyces cerevisiae. Genetics 85, 23-33 (1977).
16. Kane, P. M.: Biogenesis of the yeast vacuolar H(+)-ATPase. J. Exp. Biol. 172, 93-103 (1992).
17. Klionsky, D. J., L. M. Bant, S. D. Emr: Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information. Mol. Cell. Biol. 8, 2105-2116 (1988).
18. Klionsky, D. J., S. D. Emr: Membrane protein sorting: biosynthesis, transport and processing of yeast vacuolar alkaline phosphatase. EMBO J. 8, 2241-2250 (1989).
19. Kornfeld, S.: Structure and function of the mannose 6-phosphate/ insulin-like growth factor II receptors. Annu. Rev. Biochem. 61, 307-330 (1992).
20. Kunkel, T. A., J. D. Roberts, R. A. Zakour: Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154, 367-382 (1987).
21. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685 (1970).
22. Marcusson, E. G., B. F. Horazdorsky, J. L. Cereghino, E. Gharakhanian, S. D. Emr: The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. Cell 77, 579-586 (1994).
23. Nasmyth, K. A., S. I. Reed: Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc. Natl. Acad. Sci. USA 77, 2119-2123 (1980).
24. Nothwehr, S. F., E.Conibear, T. H. Stevens: Golgi and vacuolar membrane proteins reach the vacuole in vps1 mutant yeast cells via the plasma membrane. J. Cell Biol. 129, 35-46 (1995).
25. Nothwehr, S. F., C. J. Roberts, T. H. Stevens: Membrane protein retention in the yeast Golgi apparatus: dipeptidyl aminopeptidase A is retained by a cytoplasmic signal containing aromatic residues. J. Cell Biol. 121, 1197-1209 (1993).
26. Nothwehr, S. F., T. H. Stevens: Sorting of membrane proteins in the yeast secretory pathway. J. Biol. Chem. 269, 10185-10188 (1994).
27. Paul, R., G. W. Anderson: N,N′-carbonyldiimidazole, a new peptide forming reagent. J. Am. Chem. Soc. 82, 4596-4600 (1960).
28. Piper, R. C., A. A. Cooper, Y. Hong, T. H. Stevens: VPS27 controls vacuolar end endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae. J. Cell Biol. 131, 603-617 (1995).
29. Piper, R. C., E. A. Whitters, T. H. Stevens: Yeast Vps45p is a Sec1p-like protein required for the consumption of vacuole-targeted. post-Golgi transport vesicles. Eur. J. Cell Biol. 65, 305-318 (1994).
30. Raymond, C. K., I. HowaldStevenson, C. A. Vater, T. H. Stevens: Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol. Biol. Cell 3, 1389-1402 (1992).
31. Raymond, C. K., P. J. O'Hara, G. Eichinger, J. H. Rothman, T. H. Stevens: Molecular analysis of the yeast VPS3 gene and the role of its product in vacuolar protein sorting and vacuolar segregation during the cell cycle. J. Cell Biol. 111, 877-892 (1990).
32. Roberts, C. J., C. K. Raymond, C. T. Yamashiro, T. H. Stevens: Methods for studying the yeast vacuole. Methods Enzymol. 194, 644-661 (1991).
33. Robinson, J. S., D. J. Klionsky, L. M. Banta, S. D. Emr: Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol. Cell. Biol. 8, 4936-4948 (1988).
34. Rothman, J. H., I. Howald, T. H. Stevens: Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J. 8, 2057-2065 (1989).
35. Rothman, J. H., T. H. Stevens: Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47, 1041-1051 (1986).
36. Sanger, F., S. Nicklen, A. R. Coulson: DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467 (1977).
37. Sherman, F., G. R. Fink, J. B. Hicks, Methods in Yeast Genetics. Cold Spring Harbor Laboratory. New York 1982.
38. Sikorski, R. S., P. Hieter: A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27 (1989).
39. Stack, J. H., B. F. Horazdovsky, S. D. Emr: Receptor-mediated protein sorting to the vacuole in yeast: Roles for a protein kinase, a lipid kinase and GTP-binding proteins. Annu. Rev. Cell Dev. Biol. 11, 1-33 (1995).
40. Stevens, T., B. Esmon, R. Schekman: Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole. Cell 30, 439-448 (1982).
41. Towbin, H., T. Staehelin, J. Gordon: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354 (1979).
42. Vails, L. A., C. P. Hunter, J. H. Rothman, T. H. Stevens: Protein sorting in yeast: the localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide. Cell 48, 887-897 (1987).
43. Vater, C. A., C. K. Raymond, K. Ekena, I. Howald-Stevenson, T. H. Stevens: The VPS1 protein, a homolog of dynamin required for vacuolar protein sorting in Saccharomyces cerevisiae, is a GTPase with two functionally separable domains. J. Cell Biol. 119, 773-786 (1992).
44. Vida, T. A., G. Huyer, S. D. Emr: Yeast vacuolar proenzymes are sorted in the late-Golgi complex and transported to the vacuole via a prevacuolar endosome-like compartment. J. Cell Biol. 121, 1245-1256 (1993).
45. Wilcox, C. A., K. Redding, R. Wright, R. S. Fuller: Mutation of a tyrosine localization signal in the cytosolic tail of yeast Kex2 protease disrupts Golgi retention and results in default transport to the vacuole. Mol. Biol. Cell 3, 1353-1371 (1992).
46. Wilsbach, K., G. S. Payne: Vps1p, a member of the dynamin GTPase family, is necessary for Golgi membrane protein retention in Saccharomyces cerevisiae. EMBO J. 12, 3049-3059 (1993).
47. Wolf, D. H., G. R. Fink: Proteinase C (Carboxypeptidase Y) mutants of yeast. J. Bacteriol. 123, 1150-1156 (1975).