Quantification of in vitro growth and synthesis in rabbit middle ear epithelial cells

European Archives of Oto-Rhino-Laryngology

Volumn 253, Issue 7, 1996, Pages 395-400

  Schousboe, L P ^a   Blegvad, S ^a   Ovesen, T ^a  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

Cell culture; Glycoprotein analysis; Middle ear epithelium; Quantification

Indexed keywords

CELL PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; AUDITORY CANAL; CELL CULTURE; DNA SYNTHESIS; EPITHELIUM CELL; FEMALE; MIDDLE EAR; NONHUMAN; PRIORITY JOURNAL; RABBIT;

EID: 0029816198     PISSN: 09374477     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00168490     Document Type: Article

References (18)

1. Absher M (1973) Hemocytometer counting. In: Kruse PF, Patterson MK (eds) Tissue culture, methods and applications, 1st edn. Academic Press, New York, pp 395-397
2. Ashihara T, Baserga R (1979) Cell synchronization. In: Jakoby WB, Pastan IH (eds) Methods in enzymology, vol 58. Academic Press, New York, pp 248-261
3. BioRad (1990) Bio-Rad protein assay. Germany
4. Blitterswijk CA van, Ponce M, Muijen GN van, Wijsman MC, Koerten HK, Grote JJ (1986) Culture and characterization of rat middle-ear epithelium. Acta Otolaryngol (Stockh) 101:453-466
5. Brown DT, Litt M, Potsic WP (1985) A study of mucus glycoproteins in secretory otitis media. Arch Otolaryngol 111:688-695
6. Harada T, Kim Y, Juhn SK, Sakakura Y (1993) Biochemical characterization of mucous glycoproteins secreted by in vitro chinchilla middle ear epithelial cells. Eur Arch Otorhinolaryngol 250:182-185
7. Hawley Nelson P, Sullivan JE, Kung M, Hennings H, Yuspa SH (1980) Optimized conditions tor the growth of human epidermal cells in culture. J Invest Dermatol 75:176-182
8. Hill J, Hutton DA, Green GGR, Birchall JP, Pearson JP (1992) Culture of human middle ear mucosal explants: mucin production. Clin Otolaryngol Allied Sci 17:491-496
9. Jensen PK, Therkelsen AJ (1981) Cultivation at low temperature as a measure to prevent contamination with fibroblasts in epithelial cultures from human skin. J Invest Dermatol 77: 210-212
10. Kleinman HK, Klebe RJ, Martin GR (1981) Role of collagenous matrices in the adhesion and growth of cells. J Cell Biol 88:473-485
11. Labarca C, Paigen K (1980) A simple, rapid, and sensitive DNA assay procedure. Anal Biochem 102:344-352
12. Ledet T, Heickendorff L (1984) Glycosaminoglycans of arterial basement membrane-like material from cultured rabbit aortic myomedial cells. Biochim Biophys Acta 798:276-282
13. Nakamura A, Demaria TF, Lim DJ, Blitterswijk CA van (1991) Primary culture of chinchilla middle ear epithelium. Ann Otol Rhinol Laryngol 100:774-782
14. Ovesen T, Gaihede M, Schousboe LP, Ledet T (1994) Atmospheric air versus normal middle ear gas: the effects upon in vitro growth and collagen synthesis in normal middle ear fibroblasts. In Vitro Cell Dev Biol 30:249-255
15. Palva T (1990) The pathogenesis and treatment of cholesteatoma. Acta Otolaryngol (Stockh) 109:323-330
16. Palva T, Raunio V (1975) The role of the middle ear mucosa in secretory otitis media. J Laryngol Otol 89:491-494
17. Rago R, Mitchen J, Wilding G (1990) DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water. Anal Biochem 191:31-34
18. Schousboe LP, Ovesen T, Ottosen PD, Ledet T, Elbrönd O (1995) Culture of rabbit middle ear epithelial cells. A method for primary culture and subculture with identification, characterization and growth specification. Acta Otolaryngol (Stockh) 115:787-795