Spermatozoa selection by the swim-up procedure and two-layer Percoll gradient centrifugation

International Urology and Nephrology

Volumn 28, Issue 3, 1996, Pages 409-418

  Evliyaoglu Y ^a   Ciftci U ^b   Bozdemir, N ^c  

^a ADANA NUMUNE EDUCATION AND RESEARCH HOSPITAL   (Turkey)

^b Düzen Laboratory   (Turkey)

^c CUKUROVA UNIVERSITY   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; CELL FRACTIONATION; CELL SEPARATION; HUMAN; HUMAN CELL; MALE; MALE INFERTILITY; SPERMATOZOON; SPERMATOZOON DENSITY;

EID: 0029814953     PISSN: 03011623     EISSN: None     Source Type: Journal    
DOI: 10.1007/bf02550505     Document Type: Article

References (14)

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2. Harris, S. J., Milligan, M. P., Masson, G. M , Dennis, K. J.: Improved separation of motile sperm in asthenospermia and its application to artificial insemination homologous (AIH). Fertil. Steril., 36, 219 (1981).
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8. Morales, P., Vantman, D., Barros, C., Vigil, P.: Human spermatozoa selection by Percoll gradient or swim-up are equally capable of binding to the human zona pellucida and undergoing the acrosome reaction. Hum. Reprod., 6 (3), 401 (1991).
9. Englert, Y., Van den Bergh, M., Rodesch, C., Bertrand, E., Biramane, J., Legreve, A.: Comparative auto-controlled study between swim-up and Percoll preparation of fresh semen samples for in vitro fertilization. Hum. Reprod., 7 (3), 399 (1992).
10. Ng, F. L., Liu, D. Y., Baker, H. W.: Comparison of Percoll, mini-Percoll and swim-up methods for sperm preparation from abnormal semen samples. Hum. Reprod., 7 (2), 261 (1992).
11. Bollendorf, A., Check, J. H., Katsoff, D., Lurie, D.: Comparison of direct swim-up, mini-Percoll and Sephadex G 10 separation procedures. Arch. Androl., 32 (2), 157 (1994).
12. Guerin, J. F., Mathieu, C., Lomage, J., Pinatel, M. C., Boulieu, D.: Improvement of survival and fertilizing capacity of human spermatozoa in an IVF programme by selection on discontinuous Percoll gradients. Hum. Reprod., 4 (7), 798 (1989).
13. McClure, R. D., Nunes, L., Tom, R.: Semen manipulation, improved sperm recovery and function with a two-layer Percoll gradient. Fertil. Steril., 51 (5), 874 (1989)
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