Peptide rescue of an N-terminal truncation of the Stoffel fragment of Taq DNA polymerase

Protein Science

Volumn 5, Issue 9, 1996, Pages 1785-1792

  Vainshtein, Inna ^a   Atrazhev, Alexey ^a   Eom, Soo Hyun ^b   Elliott, John F ^a   Wishart, David S ^a   Malcolm, Bruce A ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b YALE UNIVERSITY   (United States)

Author keywords

deletion; peptide rescue; Stoffel fragment; Taq polymerase; thermostability

Indexed keywords

DNA FRAGMENT; DNA POLYMERASE; EXONUCLEASE;

AMINO ACID SUBSTITUTION; AMINO ACID SYNTHESIS; AMINO TERMINAL SEQUENCE; ARTICLE; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; GENE DELETION; NONHUMAN; PEPTIDE SYNTHESIS; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN STABILITY; PROTEIN STRUCTURE; THERMOSTABILITY;

EID: 0029814367     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050904     Document Type: Article

References (30)

1. Barnes WM. 1992. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Gene 112:29-35.
2. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-54.
3. Connelly PR, Varadarajan R, Sturtevant JM, Richards FM. 1990. Thermodynamics of protein-peptide interactions in the ribonuclease S system studied by titration calorimetry. Biochemistry 29:6108-6114.
4. Eijsink VGH, Vriend G, van den Burg B, van der Zee GR, Veltmann OR, Stulp BK, Venema G. 1992. Introduction of a stabilizing 10 residue beta-hairpin in Bacillus subtilis neutral protease. Protein Eng 5:157-162.
5. Engelke DR, Krikos A, Bruck ME, Ginsburg D. 1990. Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Anal Biochem 191:396-400.
6. Eom SH, Wang J, Steitz TA. 1996. Structure of Taq polymerase with DNA at the polymerase active site. Nature (London) 382:278-281.
7. Erlich HA, Gelfand D, Sninsky J. 1991. Recent advances in the polymerase chain reaction. Science 252:1643-1651
8. Finn FM, Hofmann K. 1965 Studies on polypeptides. XXXIII. Enzymatic properties of partially synthetic ribonucleases. J Am Chem Soc 87:645-650.
9. Hearn RP, Richards FM, Sturtevant JM, Watt GD. 1971. Thermodynamics of the binding of S-peptide to S-protein to form ribonuclease S. Biochemistry 10:806-817.
10. Johnson WC. 1990. Protein secondary structure and circular dichroism: A practical guide. Proteins Struct Funct Genet 7:205-214.
11. Kim Y, Eom SH, Wang J, Lee D, Suh SW, Steitz TA. 1995. Crystal structure of Thermus aquaticus DNA polymerase. Nature (Lond) 376:612-616.
12. Langley KE, Villarejo MR, Fowler AV, Zamenhof PJ, Zabin I. 1975. Molecular basis of beta-galactosidase alpha-complementation. Proc Natl Acad Sci USA 72:1254-1257.
13. Lawyer FC, Stoffel S, Saiki RK, Myambo K, Drummond R, Gelfand DH. 1989. Isolation, characterization and expression in Eschericia coli of the DNA polymerase gene from Thermus aquaticus. J Biol Chem 264:6427-6437.
14. Mrabet NT, van den Broeck A, van den Brande I, Stanssens P, Laroche Y, Lambeir AM, Matthijssens G, Jenkins J, Chiadmi M, van Tilbeurgh H, Rey F, Janin J, Quax WJ, Lasters I, de Maeyer M, Wodak SJ. 1992. Arginine residues as stabilizing elements in proteins. Biochemistry 31:2239-2253.
15. Nicholls A, Sharp KA, Honig B. 1991. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins Struct Funct Genet 11:281-296.
16. Nicholson H, Becktel WJ, Matthews BW. 1988. Enhanced protein thermostability from designed mutations that interact with alpha-helix dipoles. Nature (Lond) 336:651-656.
17. Numata K, Muro M, Akutsu N, Nosoh Y, Yamagishi A, Oshima T. 1995 Thermal stability of chimeric isopropylmalate dehydrogenase genes constructed from a thermophile and a mesophile. Protein Eng 8:39-43.
18. Polesky AH, Steitz TA, Grindley NDF, Joyce CM. 1990 Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Eschericia coli. J Biol Chem 265:14579-14591.
19. Provencher SW, Glockner J. 1981. Estimation of globular protein secondary structure from circular dichroism. Biochemistry 20:33-37.
20. Richards FM, Vithayathil PJ. 1959. The preparation of subtilisin-modified ribonuclease and the separation of the peptide and protein components. J Biol Chem 234:1459-1465.
21. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
22. Sanger F, Nicklen S, Coulson AR. 1977. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467.
23. Schafmeister CE, Miercke LJW, Stroud RM. 1993. Structure at 2.5 Å of a designed peptide that maintains solubility of membrane proteins. Science 262: 734-738.
24. Stark MJ. 1987. Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli. Gene 51: 255-67.
25. Tozawa K, Odaka M, Date T, Yoshida M. 1992. Molecular dissection of the beta subunit of F1-ATPase into peptide fragments. J Biol Chem 267:16484-16490.
26. Varadarajan R, Richards FM. 1992. Crystallographic structures of ribonuclease S variants with nonpolar substitutions at position 13: Packing and cavities. Biochemistry 31:12315-12327.
27. Williams KP, Shoelson SE. 1993. Cooperative self-assembly of SH2 domain fragments restores phosphopeptide binding. Biochemistry 32:11279-11284.
28. Wishart DS, Boyko RF, Willard L, Richards FM, Sykes BD. 1994. SEQSEE A comprehensive program suite for protein sequence analysis. Comput Appl Biosci 10:121-132.
29. Yang YR, Schachman HK. 1993. In vivo formation of active aspartate transcarbamoylase from complementing fragments of the catalytic polypeptide chains. Protein Sci 2:1013-1023.
30. Yanisch-Perron C, Vieira J, Messing J. 1985. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-109.