Development of a method based on alkaline gel electrophoresis for estimation of oxidative damage to DNA in Escherichia coli

Journal of Applied Bacteriology

Volumn 81, Issue 2, 1996, Pages 133-138

  Zirkle, R E ^a   Krieg, N R ^a  

^a VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; DNA; ELECTROPHORESIS; ENZYMES; REPAIR;

ALKALINES; CELL SUSPENSION; CELL-BE; CELL/B.E; DNA STRANDS; DNA-REPAIR ENZYMES; GEL ELECTROPHORESIS; ISOGENIC STRAIN; OXIDATIVE DAMAGE; STRAND BREAKAGE;

ESCHERICHIA COLI;

AGAROSE; BACTERIAL DNA; CATALASE; HYDROGEN PEROXIDE;

ANALYTIC METHOD; ARTICLE; CELL SURVIVAL; COMPUTER AIDED DESIGN; DNA DAMAGE; DNA REPAIR; DNA STRAND BREAKAGE; ESCHERICHIA COLI; GEL ELECTROPHORESIS; OXIDATIVE STRESS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029814218     PISSN: 00218847     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.1996.tb04490.x     Document Type: Article

References (15)

1. Ahnstrom, G. and Edvardsson, K.A. (1974) Radiation induced single-strand breaks in DNA determined by rate of alkaline strand separation and hydroxyapatite chromatography: an alternative to velocity sedimentation. International Journal of Radiation Research 61, 275-287.
2. Chen, C.-Z. and Sutherland, J.C. (1989) Gel electrophoresis method for the quantification of gamma ray-induced single- and doublestrand breaks in DNA irradiated in vitro. Electrophoresis 10, 318-326.
3. Demple, B. and Harrison, L. (1994) Repair of oxidative damage to DNA. Annual Review of Biochemistry 63, 915-948.
4. Demple, B., Halbrook, J. and Linn, S. (1983) Escherichia coli xth mutants are hypersensitive to hydrogen peroxide. Journal of Bacteriology 153, 1079-1082.
5. Demple, B., Johnson, A. and Fung, D. (1986) Exonuclease III and endonuclease IV remove 3 blocks from DNA synthesis primers in H2O2 damaged Escherichia coli. Proceedings of the National Academy of Sciences USA 83, 7731-7735.
6. Doetsch, P.W. and Cunningham, R.P. (1990) The enzymology of apurinic/apyrimidinic endonucleases. Mutation Research 236, 173-201.
7. Echols, H. and Goodman, M.F. (1990) Mutation induced by DNA damage: a many protein affair. Mutation Research 236, 301-311.
8. Hagensee, M.E. and Moses, R.E. (1989) Multiple pathways for the repair of hydrogen peroxide induced DNA damage in Escherichia coli. Journal of Bacteriology 171, 991-995.
9. Hutchinson, F. (1985) Chemical changes induced in DNA by ionizing radiation. Progress in Nucleic Acid Research and Molecular Ao/ogy 32, 115-154.
10. Imlay, J.A. and Linn, S. (1988) DNA damage and oxygen radical toxicity. Science 240, 1302-1309.
11. Kohn, K..W. and Ewig, R.A. (1973) Alkaline elution analysis, a new approach to the study of DNA single-strand interruptions in cells. Cancer Research 33, 1849-1853.
12. Kovacs, E. and Langemann, H. (1990) Investigation of the repair of single-strand breaks in human DNA using alkaline gel electrophoresis. Radiation Research 124, 137-140.
13. Livneh, Z., Cohen-Fix, O., Skaliter, R. and Elizur, T. (1993) Replication of damaged DNA and the molecular mechanism of ultraviolet light mutagenesis. Critical Review of Biochemistry and Molecular Biology 28, 465-513.
14. McGrath, R.A. and Williams, R.W. (1966) Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces. Nature 212, 534-535.
15. Zhang, Q.-M., Yonei, S. and Kato, M. (1992) Multiple pathways for repair of hydrogen peroxide induced DNA damage in Escherichia coli. Mutation Research 132, 334-388.