A single amino acid determines differences in ethanol actions on strychnine-sensitive glycine receptors

Molecular Pharmacology

Volumn 50, Issue 2, 1996, Pages 402-406

  Mascia, Maria Paola ^a   Mihic, S John ^a   Valenzuela, C Fernando ^a   Schofield, Peter R ^c   Harris, R Adron ^a,b  

^a UNIVERSITY OF COLORADO   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

^c GARVAN INSTITUTE OF MEDICAL RESEARCH   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; GLYCINE RECEPTOR; PROPOFOL; RECEPTOR SUBUNIT; STRYCHNINE;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CONCENTRATION RESPONSE; DRUG RECEPTOR BINDING; FEMALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; RECEPTOR AFFINITY; RECEPTOR UPREGULATION; XENOPUS LAEVIS;

ANIMALS; CHLORIDES; DOSE-RESPONSE RELATIONSHIP, DRUG; ETHANOL; FEMALE; GLYCINE; GLYCINE AGENTS; MICE; RECEPTORS, GLYCINE; STRUCTURE-ACTIVITY RELATIONSHIP; STRYCHNINE; XENOPUS LAEVIS;

EID: 0029814174     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Betz, H. Glycine receptors: heterogeneous and widespread in the mammalian brain. Trends Neurosci. 14:458-461 (1991).
2. Grenningloh, G., E. Gundelfinger, B. Schmitt, H. Betz, M. G. Darlison, E. B. Barnard, P. R. Schofield, and P. H. Seeburg. Glycine vs GABA receptors. Nature (Lond.) 330:25-26 (1987).
3. Vandenberg, R. J., C. R. French, P. H. Barry, J. Shine, and P. R. Schofield. Antagonism of ligand-gated ion channel receptors: two domains of the glycine receptor α subunit form the strychnine-binding site. Proc. Natl. Acad. Sci. USA 89:1765-1769 (1992).
4. Vandenberg, R. J., C. A. Handford, and P. R. Schofield. Distinct agonist-and antagonist-binding sites on the glycine receptor. Neuron 9:491-496 (1992)
5. Grenningloh, G., V. Schmieden, P. R. Schofield, P. H. Seeburg, T. Siddique, T. K. Mohandas, C.-M. Becker, and H. Betz. Alpha subunit variants of the human glycine receptor: primary structures, functional expression and chromosomal localization of the corresponding genes. EMBO J. 9:771-776 (1990).
6. Grenningloh, G., I. Pribilla, P. Prior, G. Multhaup, K. Beyreuther, O. Taleb, and H. Betz. Cloning and expression of the 58 kd γ subunit of the inhibitory glycine receptor. Neuron 4:963-970 (1990).
7. Kuhse, J., V. Schmieden, and H. Betz. Identification and functional expression of a novel ligand binding subunit of the inhibitory glycine receptor. J. Biol. Chem. 265:22317-22320 (1990).
8. Malosio, M.-L., B. Marqueze-Pouey, J. Kuhse, and H. Betz. Widespread expression of glycine receptor subunit mRNAs in the adult and developing rat brain. EMBO J. 10:2401-2409 (1991).
9. Rajendra, S., and P. R. Schofield. Molecular mechanism of inherited startle syndromes. Trends Neurosci. 18:80-82 (1995).
10. Bormann, J., N. Rundstrom, H. Betz, and D. Langosch. Residues within transmembrane segment M2 determine chloride conductance of glycine receptor homo- and hetero-oligomers. EMBO J. 12:3729-3737 (1993).
11. Sanna, E., and R. A. Harris. Neuronal ion channels. Recent Dev. Alcohol. 11:169-186 (1993).
12. A receptor/ chloride channel complex, in Pharmacological Effects of Ethanol on the Nervous System (R. A. Deitrich and V. G. Erwin, eds.). CRC Press, New York (1996).
13. Williams, K. L., A. P. Ferko, E. J. Barbieri, and G. J. Di Gregorio. Glycine enhances the central depressant properties of ethanol in mice. Pharmacol. Biochem. Behav. 50:199-205 (1995).
14. - currents in cultured mouse neurons. J. Pharmacol. Exp. Ther. 270:61-69 (1994).
15. Celentano, J. J., T. T. Gibbs, and D. H. Farb. Ethanol potentiates GABA and glycine induced currents in chick spinal cord neurons. Brain Res. 455:377-380 (1988).
16. - fluxes in rat brain synaptoneurosomes. J. Neurochem. 57:384-390 (1991).
17. Sontheimer, H., C.-M Becker, D. B. Pritchett, P. R. Schofield, G. Grenningloh, H. Kettenmann, H. Betz, and P. H. Seeburg. Functional chloride channels by mammalian cell expression of rat glycine receptor subunit. Neuron 2:1491-1497 (1989)
18. Ryan, S. G., M. S. Buckwalter, J. W. Lynch, C. A. Handford, L. Segura, R. Shiang, J. J. Wasmuth, S. A. Camper, P. R. Schofield, and P. O'Connell. A missense mutation in the gene encoding the α1 subunit of the inhibitory glycine receptor in the spasmodic mouse. Nature Genet. 7:131-135 (1994)
19. Lin, L.-H., L. L. Chen, J. A. Zirrolli, and R. A. Harris. General anesthetics potentiate GABA actions on GABAA receptors expressed by Xenopus oocytes: lack of involvement of intracellular calcium. J. Pharmacol. Exp. Ther. 263:569-578 (1992).
20. Colman, A. Expression of exogenous DNA in Xenopus oocytes, in Transcription and Translation: A Practical Approach (B. D. Hames and S. J. Higgins, eds.). Oxford Press, Washington, D.C. (1984).
21. Taleb, O., and H. Betz. Expression of the human glycine receptor α1 subunit in Xenopus oocytes: apparent affinities of agonists increase at high receptor density. EMBO J. 13:1318-1324 (1994).
22. Rundstrom, N., V. Schmieden, H. Betz, J. Bormann, and D. Langosch. Cyanotriphenylborate: subtype-specific blocker of glycine receptor chloride channels. Proc. Natl. Acad. Sci. USA 81:8950-8954 (1994).
23. Saul, B., V. Schmieden, C. Kling, C. Mulhardt, P. Gass, J. Kuhse, and C.-M. Becker. Point mutation of glycine receptor α1 subunit in the spasmodic mouse affects agonist responses. FEBS Lett. 350:71-76 (1994).
24. A responses in cells stably transfected with receptor subunits. Alcohol. Clin. Exp. Res. 19:226-232 (1995).
25. A receptor-mediated currents: lack of subunit specificity. Eur. J. Pharmacol. 268:209-214 (1994).
26. A receptors requires phosphorylation of the alternatively spliced variant of the gamma 2 subunit. FEBS Lett. 313:113-117 (1992)
27. Garnier, J., D. G. Osguthorpe, and B. Robson. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J. Mol. Biol. 120:97-120 (1978).