Cysteine-scanning mutagenesis of transmembrane domain XII and the flanking periplasmic loop in the lactose permease of Escherichia coli

Biochemistry

Volumn 35, Issue 39, 1996, Pages 12909-12914

  He, Molly M ^a   Sun, Jianzhong ^a   Ronald Kaback, H ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; LACTOSE; LACTOSE PERMEASE; MUTANT PROTEIN; N ETHYLMALEIMIDE;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GROWTH; CARBOHYDRATE TRANSPORT; ENZYME INACTIVATION; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE MUTATION; IMMUNOBLOTTING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; CELL MEMBRANE; CYSTEINE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; ETHYLMALEIMIDE; LACTOSE; MEMBRANE TRANSPORT PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, SECONDARY; SYMPORTERS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029813985     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi960876b     Document Type: Article

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