Development of an immunoradiometric assay for quantitative determination of CrylA(b) protein in transgenic sugarcane plants

Journal of Immunological Methods

Volumn 196, Issue 1, 1996, Pages 33-39

  Vázquez, Roberte I ^a   Prieto, Dmitri ^a   De La Riva, Gustavo A ^a   Selman Housein, Guillermo ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Bacillus thuringiensis; ELISA; Expression level; Immunoassay; Immunoradiometric assay; Endotoxin

Indexed keywords

CRYSTAL PROTEIN; ENDOTOXIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNORADIOMETRIC ASSAY; PRIORITY JOURNAL; TRANSGENIC PLANT;

BACILLUS THURINGIENSIS; SACCHARUM HYBRID CULTIVAR;

EID: 0029813730     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(96)00100-7     Document Type: Article

References (20)

1. Adang, M.J., Brody, M.S., Cardineau, G., Eagen, N., Roush, R.T., Shewmaker, S.K., Jones, A., Oakes, J.V. and McBride, K.E. (1993) The reconstruction and expression of a Bacillus thuringiensis cryIIIA gene in protoplasts and potato plants. Plant Mol. Biol. 21, 1131-1145.
2. Arencibia, A., Molina, R., Gutiérrez, C., Greenidge, V., Menéndez, E., De la Riva, G.A and Selman-Housein, G. (1992) Regeneration of transgenic sugarcane (Saccharum officinarum L.) plants from intact meristenatic tissue transformed by electroporation. Biotecnol. Apl. 9, 156-165.
3. Arencibia, A., Molina, P., De la Riva, G.A. and Selman-Housein, G. (1995) Production of transgenic sugarcane (Saccharum officinarum L.) plants by intact cell electroporation. Plant Cell Rep. 14, 305-309.
4. Arencibia, A., Vázquez, R.I., Prieto, D., Téllez, P., Carmona, E.R., Coego, A., Hernández, L., de la Riva, G.A. and Selman-Housein, G. (1996) Transgenic sugarcane plants resistant to stem borer attack. Sumitted to Mol. Breeding.
5. Bradford, M.M. (1976) A rapid and sensitive method for the quantificaton of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
6. Feitelson, J.S., Payne, J. and Kim, L. (1992) Bacillus thuringiensis: insects and beyond. Bio/Technology 10, 271-275.
7. Fischhoff, D.A., Bowdish, K.S., Perlak, F.J., Marrone, P.G., McCormick, M.S., Niedermeyer, J.D., Dean, D.A., Kusano-Kretzmer, K., Mayer, E.J., Rochester, D.E., Rogers, S.G. and Fraley, R.T. (1987) Insect resistant transgenic tomato plants. Bio/Technology 5, 807-813.
8. Hofmann, C., Lüthy, P., Hütter, R. and Pliska, V. (1988) Binding of the δ-endotoxin from Bacillus thuringiensis to brush-border membrane vesicles of cabbage butterfly (Pieris brassicae). Env. J. Biochem. 173, 85-91.
9. Höfte, H. and Whiteley, H. (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53, 242-255.
10. Hunter, W.M. and Greenwood, F. (1962) Preparation of iodine-131 labelled human growth hormone of specific activity. Nature 194, 495-496.
11. Kaniewski, W.K. and Thomas, P.E. (1988) A two-step ELISA for rapid, reliable detection of potato viruses. Am. Potato J. 5, 561-571.
12. Knowles, B.H. and Dow, J.A.T. (1993) The crystal δ-endotoxins of Bacillus thuringiensis: models for their mechanism of action on the insect gut. Bioessays 15, 83-91.
13. Koziel, M.G., Beland, G.L., Bowman, C., Carrozzi, N.B., Crenshaw, R., Crossland, L., Dawson, J., Desai, N., Hill, M.S., Kadwell, S., Launis, K., Lewis, K., Maddox, D., McPherson, K., Meghji, M.R., Merlin, E., Rhodes, R., Warren, G.W., Wright, M. and Evola, S.V. (1993) Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Bio/Technology 11, 194-200.
14. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
15. Perlack, F.J., Deaton, R.W., Armstrong, T.A., Fuch, R.T., Sims, S.R., Greenplate, J.T. and Fischhoff, D.A. (1990) Insect resistant cotton plants. Bio/Technology 8, 939-943.
16. Vaeck, M., Reynaerts, A., Höfte, H., Jansens, S., De Beukeleer, M., Dean, D.A., Zabeau, M., Van Montagu, M. and Leemans, J. (1987) Transgenic plants protected from insect attack. Nature 328, 33-37.
17. N in tobacco plants transformed with the viral coat protein cistron. Plant Mol. Biol. 20, 631-639.
18. Van der Wilk, F., Posthumus-Lutke, W.P. and Goldbach, R. (1991) Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection. Plant Mol. Biol. 17, 431-439.
19. Vázquez, R.I., Prieto, D., Oloriz, M.I., De la Riva, G.A. and Selman-Housein, G. (1995) Heterologous expression of recombinant δ-endotoxins from Bacillus thuringiensis in Escherichia coli: comparative study of three transcriptional systems. Rev. Lat.-amer. Microbiol. 37, 237-244.
20. Voller, A., Bidwell, D. and Barlett, A. (1977) Enzyme Linked Immunosorbent Assay (ELISA). A Review with a Bibliography of Microplates Application. Flowgene Publication, Guernsey.