Replication protein-A mediates the association of calf thymus DNA polymerase α-DNA primase complex with guanine-rich DNA sequence

Journal of Biochemistry

Volumn 120, Issue 4, 1996, Pages 766-772

  Suzuki, Motoshi ^a   Tamiya Koizumi, Keiko ^a   Takemura, Masaharu ^a   Furuta, Keigo ^a   Izuta, Shunji ^a   Savoysky, Ericka ^b   Miura, Ayuko ^a   Yoshida, Shonen ^a  

^a NAGOYA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b CHUGAI PHARMACEUTICAL CO LTD   (Japan)

Author keywords

DNA polymerase primase; DNA replication; Replication protein A

Indexed keywords

DNA DIRECTED DNA POLYMERASE ALPHA; DNA PRIMASE; PROTEIN A;

ARTICLE; DNA REPLICATION; DNA SEQUENCE; DNA STRUCTURE; DNA SYNTHESIS; ESCHERICHIA COLI; NONHUMAN; SIMIAN VIRUS 40; THYMUS;

ESCHERICHIA COLI; SIMIAE; SIMIAN VIRUS; SIMIAN VIRUS 40;

EID: 0029812212     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021477     Document Type: Article

References (38)

1. Kornberg, A. and Baker, T. (1992) DNA Replication, W.H. Freeman and Company, New York
2. Conaway, R.C. and Lehman, I.E. (1982) A DNA primase activity associated with DNA polymerase α from Drosophila melanogaster embryos. Proc. Natl. Acad. Sci. USA 79, 4585-4588
3. Shioda, M., Nelson, E.M., Bayne, M.L., and Benbow, R.M. (1982) DNA primase activity associated with DNA polymerase α from Xenopus laevis ovaries. Proc. Natl. Acad. Sci. USA 79, 7209-7213
4. Yagura, T., Kozu, T., and Seno, T. (1982) Mouse DNA polymerase accompanied by a novel RNA polymerase activity: Purification and partial characterization. J. Biochem. 91, 607-618
5. Suzuki, M., Izuta, S., Savoysky, E., Sakurai, T., Simbulan, C., Tatebe, M., Kojima, K., and Yoshida, S. (1993) Deoxypyrimidine cluster mediates the priming by calf thymus DNA primase subunit. Biochem. Mol. Biol. Int. 29, 645-652
6. Suzuki, M., Savoysky, E., Izuta, S., Tatebe, M., Okajima, T., and Yoshida, S. (1993) RNA priming coupled with DNA synthesis on natural template by calf thymus DNA polymerase α-primase. Biochemistry 32, 12782-12792
7. Collins, K.L. and Kelly, T.J. (1991) Effects of T antigen and replication protein A on the initiation of DNA synthesis by DNA polymerase α-primase. Mol. Cell. Biol. 11, 2108-2115
8. Savoysky, E., Suzuki, M., Simbulan, C., Tamai, K., Ohuchi, T., Akiyama, T., and Yoshida, S. (1993) Immunopurified Rb protein inhibits SV40 T antigen-dependent stimulation of DNA polymerase α. Oncogene 8, 319-325
9. Holmes, A.M., Cheriathundam, E., Bollum, F.J., and Chang, L.M. (1985) Initiation of DNA synthesis by the calf thymus DNA polymerase-primase complex. J. Biol. Chem. 260, 10840-10846
10. Yoshida, S., Suzuki, R., Masaki, S., and Koiwai, O. (1985) Arabinosylnucleoside 5′-triphosphate inhibits DNA primase of calf thymus. J. Biochem. 98, 427-433
11. Kuchta, R.D., Reid, B., and Chang, L.M. (1990) DNA primase. Processivity and the primase to polymerase α activity switch. J. Biol. Chem. 265, 16158-16165
12. Podust, V.N., Vladimirova, O.V., Manakova, E.N., and Lavrik, O.I. (1991) Eukaryotic DNA primase. Abortive synthesis of oligoadenylates. FEBS Lett. 280, 281-283
13. Harrington, C. and Perrino, F.W. (1995) Initiation of RNA-primed DNA synthesis in vitro by DNA polymerase alpha-primase, Nucleic Acids Res. 23, 1003-1009
14. Roth, Y.F. (1987) Eucaryotic primase. Eur. J. Biochem. 165, 473-481
15. Yamaguchi, M., Hendrickson, E.A., and DePamphilis, M.L. (1985) DNA primase-DNA polymerase α from simian cells: Sequence specificity of initiation sites on simian virus 40 DNA. Mol. Cell. Biol. 5, 1170-1183
16. Davey, S.K. and Faust, E.A. (1990) Murine DNA polymerase α fills gaps to completion in a direct assay. Altered kinetics of de novo DNA synthesis at single nucleotide gaps. J. Biol. Chem. 265, 4098-4104
17. Sheaff, K.J. and Kuchta, R.D. (1993) Mechanism of calf thymus DNA primase: Slow initiation, rapid polymerization, and intelligent termination. Biochemistry 32, 3027-3037
18. Smale, S.T. and Tjian, R. (1986) T-antigen DNA polymerase a complex implicated in simian virus 40 DNA replication. Mol. Cell. Biol. 6, 4077-4087
19. Cannon, J.V. and Lane, D.P. (1987) p53 and DNA polymerase α compete for binding to SV40 T antigen. Nature 329, 456-458
20. Dornreiter, I., Hoss, A., Arthur, A.K., and Fanning, E. (1990) SV40 T antigen binds directly to the large subunit of purified DNA polymerase α. EMBO. J. 9, 3329-3336
21. Collins, K.L., Russo, A.A.R., Tseng, B.Y., and Kelly, T.J. (1993) The role of the 70 kDa subunit of human DNA polymerase α in DNA replication. EMBO J. 12, 4555-4566
22. Dornreiter, I., Copeland, W.C., and Wang, T.S. (1993) Initiation of simian virus 40 DNA replication requires the interaction of a specific domain of human DNA polymerase α with large T antigen. Mol. Cell. Biol. 13, 809-820
23. Dornreiter, I., Erdile, L.F., Gilbert, I.U., von Winkler, D., Kelly, T.J., and Fanning, E. (1992) Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen. EMBO J. 11, 769-776
24. Hay, R.T. and DePamphilis, M.L. (1982) Initiation of SV40 DNA replication in vivo: Location and structure of 5′ ends of DNA synthesized in the on region. Cell 28, 767-779
25. Ishimi, Y., Claude, A., Bullock, P., and Hurwitz, J. (1988) Complete enzymatic synthesis of DNA containing the SV40 origin of replication. J. Biol. Chem. 263, 19723-19733
26. Savoysky, E., Suzuki, M., Tamai, K., Sakurai, T., Ohuchi, T., Akiyama, T., Kojima, K., and Yoshida, S. (1992) Efficient purification of a full length and biochemically active p110Rb, the retinoblastoma gene product. Biochem. Biophys. Res. Commun. 187, 697-702
27. Kenny, M.K., Schlegel, U., Furneaux, H., and Hurwitz, J. (1990) The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication. J. Biol. Chem. 265, 7693-7700
28. Suzuki, M., Izuta, S., and Yoshida, S. (1994) DNA polymerase α overcomes an error-prone pause site in the presence of replication protein-A. J. Biol. Chem. 269, 10225-10228
29. Masaki, S., Shiku, H., Kaneda, T., Koiwai, O., and Yoshida, S. (1982) Production and characterization of monoclonal antibody against 10S DNA polymerase α from calf thymus. Nucleic Acids Res. 10, 4703-4713
30. Tamai, K., Kojima, K., Hanaichi, T., Masaki, S., Suzuki, M., Umekawa, H., and Yoshida, S. (1988) Structural study of immunoaffinity-purified DNA polymerase α-DNA primase complex from calf thymus. Biochim. Biophys. Acta 860, 263-273
31. Fairman, M., Prelich, G., Tsurimoto, T., and Stillman, B. (1988) Identification of cellular components required for SV40 DNA replication in vitro. Biochim. Biophys. Acta 951, 382-387
32. Kenny, M.K., Lee, S.H., and Hurwitz, J. (1989) Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: Single-strand stabilization and stimulation of DNA polymerases α and δ. Proc. Natl. Acad. Sci. USA 86, 9757-9761
33. Wold, M.S., Weinberg, D.H., Virahup, D.M., Li, J.J., and Kelly, T.J. (1989) Identification of cellular proteins required for simian virus 40 DNA replication. J. Biol. Chem. 264, 2801-2809
34. Kim, C., Paulus, B.F., and Wold, M.S. (1994) Interactions of human replication protein A with oligonucleotides, Biochemistry 33, 14197-14206
35. Kim, C., Snyder, R.O., and Wold, M.S. (1992) Binding properties of replication protein A from human and yeast cells. Mol. Cell. Biol. 12, 3050-3059
36. Bullock, P.A. and Denis, D. (1995) DNA synthesis generally initiates outside the simian virus 40 core origin in vitro. Mol. Cell. Biol. 15, 173-178
37. Carty, M.P., Levine, A.S., and Dixon, K. (1992) HeLa cell single-stranded DNA-binding protein increases the accuracy of DNA synthesis by DNA polymerase δ in vitro. Mutat. Res. 274, 29-43
38. Simbulan, C.M.G., Tamiya-Koizumi, K., Suzuki, M., Shoji, M., Taki, T., and Yoshida, S. (1994) Sphingosine inhibits the synthesis of RNA primers by primase in vitro. Biochemistry 33, 9007-9012