High-resolution analysis of immunoglobulin heavy-chain gene rearrangements using denaturing gradient gel electrophoresis

Diagnostic Molecular Pathology

Volumn 5, Issue 3, 1996, Pages 159-165

  Tierens, A ^a   Lozano, M D ^a   Wickert, R ^a   Chan, W C ^a   Greiner, T C ^a  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

Denaturing gradient gel electrophoresis; Immunoglobulin gene rearrangements; Lymphoma; Polymerase chain reaction

Indexed keywords

IMMUNOGLOBULIN HEAVY CHAIN;

ARTICLE; B CELL LYMPHOMA; DENATURING GRADIENT GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE REARRANGEMENT; HUMAN; HUMAN TISSUE; IMMUNOGENETICS; IMMUNOGLOBULIN GENE; MINIMAL RESIDUAL DISEASE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ANTIBODIES, MONOCLONAL; BASE SEQUENCE; CLONING, MOLECULAR; ELECTROPHORESIS, AGAR GEL; GENE REARRANGEMENT, B-LYMPHOCYTE; GENES, IMMUNOGLOBULIN; HUMANS; IMMUNOGLOBULIN HEAVY CHAINS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 0029811894     PISSN: 10529551     EISSN: None     Source Type: Journal    
DOI: 10.1097/00019606-199609000-00003     Document Type: Article

References (24)

1. Bourguin A, Tung R, Galili N, Sklar J. Rapid nonradioactive detection of clonal T-cell receptor gene rearrangements in lymphoid neoplasms. Proc Natl Acad Sci USA 1990;87: 8536-40.
2. Cayuela JM, Herbert J, Sigaux F. Correspondence. Am J Pathol 1994;145:498-9.
3. Chan WC, Hooper C, Wickert R, et al. HTLV-I sequence in lymphoproliferative disorders. Diagn Mol Pathol 1993;2: 192-6.
4. Cleary ML, Meeker TC, Levy S, et al. Clustering of extensive somatic mutations in the variable region of an immunoglobulin heavy chain gene from a human B cell lymphoma. Cell 1986;44:97-106.
5. Davis TH, Courtland EY, Balk SP. Detection of clonal immunoglobulin gene rearrangements by polymerase chain reaction amplification and single-strand conformational polymorphism analysis. Am J Pathol 1993;142:1841-7.
6. Deane M, Norton JD. Immunoglobulin gene fingerprinting: an approach to analysis of B lymphoid clonality in lymphoproliferative disorders. Br J Haematol 1991;77:274-81.
7. Greiner TC, Raffeld M, Lutz C, Dick F, Jaffe ES. Analysis of T-cell receptor γ gene rearrangements by denaturing gradient gel electrophoresis of GC-clamped PCR products: correlation with tumor-specific sequences. Am J Pathol 1995; 146:46-55.
8. Inghirami G, Szabolics MJ, Yee HT, Corradini P, Cesarman E, Knowles DM. Detection of immunoglobulin gene rearrangement of B cell non-Hodgkin's lymphoma and leukemias in fresh, unfixed and formalin-fixed, paraffinembedded tissue by polymerase chain reaction. Lab Invest 1993;68:746-57.
9. Lozano MD, Tierens A, Greiner TC, Wickert RS, Weisenburger DD, Chan WC. Clonality analysis of B-lymphoid proliferations using PCR. Cancer 1996;77:1349-55.
10. Myers RM, Fischer SG, Lerman LS, Maniatis T. Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis Nucleic Acids Res 1985;13:3135-45.
11. Myers RM, Maniatis T, Lerman LS. Detection and localization of single base changes by denaturing gradient gel electrophoresis. Methods Enzymol 1987;155:501-27.
12. Myers RM, Sheffield VC, Cox DR. Mutation detection by PCR, GC-clamps, and denaturing gradient gel electrophoresis. In: Erlich HA, ed. PCR technology: principles and applications for DNA amplification. New York: Stockton Press, 1990:71-88.
13. Reed TJ, Reid A, Wallberg K, O'Leary TJ, Frizzera G. Determination of B-cell clonality in paraffin-embedded lymph nodes using the polymerase chain reaction. Diagn Mol Pathol 1993;2:42-9.
14. Sheffield VC, Cox DR, Lerman LS, Myers RM. Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc Natl Acad Sci USA 1989;6:2232-6.
15. Sioutos N, Bagg A, Michaud GY, et al. Polymerase chain reaction versus Southern blot hybridization. Detection of immunoglobulin heavy-chain gene rearrangements. Diagn Mol Pathol 1995;41:8-13.
16. Slack DN, McCarthy KP, Wiedemann LM, Sloane JP. Evaluation of sensitivity, specificity, and reproducibility of an optimized method for detecting clonal rearrangements of immunoglobulin and T-cell receptor genes in formalin-fixed, paraffin embedded sections. Diagn Mol Pathol 1993;2:223-32.
17. Theodorou I, Delfau-Larue M-H, Bigorgne C, et al. Cutaneous T-cell infiltrates: analysis of T-cell receptor γ gene rearrangement by polymerase chain reaction and denaturing gradient gel electrophoresis. Blood 1995;86:305-10.
18. Tonegawa S. Somatic generation of antibody diversity. Nature 1983;302:575-81.
19. Trainor KJ, Brisco MJ, Wan JH, Neoh S, Grist S, Morley AA. Gene rearrangement in B- and T-lymphoproliferative disease detected by the polymerase chain reaction. Blood 1991;78:192-6.
20. Waldmann TA. The arrangement of Ig and T-cell receptor genes in human proliferative disorders. Adv Immunol 1987; 40:247-321.
21. Wan JH, Trainor KJ, Brisco MJ, Morely AA. Monoclonality in B cell lymphoma detected in paraffin wax embedded sections using the polymerase chain reaction. J Clin Pathol 1990;43:888-90.
22. Wickert RS, Weisenburger DD, Tierens A, Greiner TC, Chan WC. Clonal relationship between lymphocytic predominance Hodgkin's disease and concurrent or subsequent large cell lymphoma of B-lineage. Blood 1995;86:2312-20.
23. Wright DK, Manos MM. Sample preparation from paraffinembedded tissues. In: Innis MA, Gelfand DA, Sninsky JJ, White TJ, eds. PCR protocols. A guide to methods and applications. San Diego, CA: Academic Press, 1990:153.
24. Yamada M, Hudson S, Tournay O, et al. Detection of minimal disease in hematopoietic malignancies of the B-cell lineage by using third-complementarity-determining region (CDR-III)-specific probes. Proc Natl Acad Sci USA 1989; 86:5123-7.