Isolation of multiple mRNAs from a few eukaryotic cells: A fast method to obtain templates for RT-PCR

BioTechniques

Volumn 21, Issue 3, 1996, Pages 394-396

  Noppen, Christoph ^a   Spagnoli, Giulio C ^a   Schaefer, Christoph ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

ARTICLE; GENE ISOLATION; MOLECULAR CLONING; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PURIFICATION; YEAST;

EUKARYOTA;

EID: 0029810990     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96213bm11     Document Type: Article

References (5)

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2. Coulie, P., V. Brichard, A. Van Pel, T. Wölfel, J. Schneider, C. Traversari, S. Mattei, E. De Plaen, C. Lurquin, J.P. Szikora, J.C. Renauld and T. Boon. 1994. A new gene coding for differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas. J. Exp. Med. 180:35-42.
3. Hoon, S.B., Y. Wang, P.S. Dale, A.C. Conrad, P. Schmid, D. Garrison, C. Kuo, L.J. Foshag, A.J. Nizze and D.L. Morton. 1995. Detection of occult melanoma cells in blood with a multiple-marker Polymerase chain reaction assay. J. Clin. Onc. 13:2109-2116.
4. Houghton, A.N. 1994. Cancer antigens: immune recognition of self and altered self. J. Exp. Med. 180:1-4.
5. Kawakami, Y., S. Eliyahu, K. Sakaguchi, P.F. Robbins, L. Rivoltini, J.R. Yannelli, E. Appella and S.A. Rosenberg. 1994. Identification of the immunodominant peptides of the MART-1 human melanoma antigen recognized by the majority of HLA-A2-restricted tumor infiltrating lymphocytes. J. Exp. Med. 180:347-352.