-
1
-
-
0019994630
-
-
R. Montesano, J. Roth, A. Robert, L. Orci, Nature 296, 651 (1982); D. Trans, J.-L. Carpentier, F. Sawano, P. Gorden, L. Orci, Proc. Natl. Acad. Sci. U.S.A. 84, 7957 (1987); R. G. Parton, J. Histochem. Cytochem. 42, 155 (1994).
-
(1982)
Nature
, vol.296
, pp. 651
-
-
Montesano, R.1
Roth, J.2
Robert, A.3
Orci, L.4
-
2
-
-
0023449563
-
-
R. Montesano, J. Roth, A. Robert, L. Orci, Nature 296, 651 (1982); D. Trans, J.-L. Carpentier, F. Sawano, P. Gorden, L. Orci, Proc. Natl. Acad. Sci. U.S.A. 84, 7957 (1987); R. G. Parton, J. Histochem. Cytochem. 42, 155 (1994).
-
(1987)
Proc. Natl. Acad. Sci. U.S.A.
, vol.84
, pp. 7957
-
-
Trans, D.1
Carpentier, J.-L.2
Sawano, F.3
Gorden, P.4
Orci, L.5
-
3
-
-
0028057494
-
-
R. Montesano, J. Roth, A. Robert, L. Orci, Nature 296, 651 (1982); D. Trans, J.-L. Carpentier, F. Sawano, P. Gorden, L. Orci, Proc. Natl. Acad. Sci. U.S.A. 84, 7957 (1987); R. G. Parton, J. Histochem. Cytochem. 42, 155 (1994).
-
(1994)
J. Histochem. Cytochem.
, vol.42
, pp. 155
-
-
Parton, R.G.1
-
4
-
-
0018951216
-
-
C. Huet, J. F. Ash, S. J. Singer, Cell 21, 429 (1980); G. Raposo et al., Eur. J. Cell Biol. 50, 340 (1989); R. I. Goldberg, R. M. Smith, L. Jarett, J. Cell Physiol. 133, 203 (1987); G. E. Palade and R. R. Bruns, J. Cell Biol. 37, 633 (1968); L. Ghitescu, A. Fixman, M. Simionescu, N. Simionescu, ibid. 102, 1304 (1986).
-
(1980)
Cell
, vol.21
, pp. 429
-
-
Huet, C.1
Ash, J.F.2
Singer, S.J.3
-
5
-
-
0024804424
-
-
C. Huet, J. F. Ash, S. J. Singer, Cell 21, 429 (1980); G. Raposo et al., Eur. J. Cell Biol. 50, 340 (1989); R. I. Goldberg, R. M. Smith, L. Jarett, J. Cell Physiol. 133, 203 (1987); G. E. Palade and R. R. Bruns, J. Cell Biol. 37, 633 (1968); L. Ghitescu, A. Fixman, M. Simionescu, N. Simionescu, ibid. 102, 1304 (1986).
-
(1989)
Eur. J. Cell Biol.
, vol.50
, pp. 340
-
-
Raposo, G.1
-
6
-
-
0023518564
-
-
C. Huet, J. F. Ash, S. J. Singer, Cell 21, 429 (1980); G. Raposo et al., Eur. J. Cell Biol. 50, 340 (1989); R. I. Goldberg, R. M. Smith, L. Jarett, J. Cell Physiol. 133, 203 (1987); G. E. Palade and R. R. Bruns, J. Cell Biol. 37, 633 (1968); L. Ghitescu, A. Fixman, M. Simionescu, N. Simionescu, ibid. 102, 1304 (1986).
-
(1987)
J. Cell Physiol.
, vol.133
, pp. 203
-
-
Goldberg, R.I.1
Smith, R.M.2
Jarett, L.3
-
7
-
-
0014301569
-
-
C. Huet, J. F. Ash, S. J. Singer, Cell 21, 429 (1980); G. Raposo et al., Eur. J. Cell Biol. 50, 340 (1989); R. I. Goldberg, R. M. Smith, L. Jarett, J. Cell Physiol. 133, 203 (1987); G. E. Palade and R. R. Bruns, J. Cell Biol. 37, 633 (1968); L. Ghitescu, A. Fixman, M. Simionescu, N. Simionescu, ibid. 102, 1304 (1986).
-
(1968)
J. Cell Biol.
, vol.37
, pp. 633
-
-
Palade, G.E.1
Bruns, R.R.2
-
8
-
-
0022551377
-
-
C. Huet, J. F. Ash, S. J. Singer, Cell 21, 429 (1980); G. Raposo et al., Eur. J. Cell Biol. 50, 340 (1989); R. I. Goldberg, R. M. Smith, L. Jarett, J. Cell Physiol. 133, 203 (1987); G. E. Palade and R. R. Bruns, J. Cell Biol. 37, 633 (1968); L. Ghitescu, A. Fixman, M. Simionescu, N. Simionescu, ibid. 102, 1304 (1986).
-
(1986)
J. Cell Biol.
, vol.102
, pp. 1304
-
-
Ghitescu, L.1
Fixman, A.2
Simionescu, M.3
Simionescu, N.4
-
9
-
-
0023605609
-
-
A. J. Milici, N. E. Watrous, H. Stukenbrok, G. E. Palade, J. Cell Biol. 105, 2603 (1987); L Ghitescu and M. Bendayan, ibid. 117, 745 (1992).
-
(1987)
J. Cell Biol.
, vol.105
, pp. 2603
-
-
Milici, A.J.1
Watrous, N.E.2
Stukenbrok, H.3
Palade, G.E.4
-
10
-
-
0026649889
-
-
A. J. Milici, N. E. Watrous, H. Stukenbrok, G. E. Palade, J. Cell Biol. 105, 2603 (1987); L Ghitescu and M. Bendayan, ibid. 117, 745 (1992).
-
(1992)
J. Cell Biol.
, vol.117
, pp. 745
-
-
Ghitescu, L.1
Bendayan, M.2
-
11
-
-
0027997787
-
-
J. E. Schnitzer, P. Oh, E. Pinney, A. Allard, ibid. 127, 1217 (1994).
-
(1994)
J. Cell Biol.
, vol.127
, pp. 1217
-
-
Schnitzer, J.E.1
Oh, P.2
Pinney, E.3
Allard, A.4
-
15
-
-
0027931859
-
-
_ and P. Oh, J. Biol. Chem. 269, 6072 (1994); Am. J. Physiol. 263, H1872 (1992).
-
(1994)
J. Biol. Chem.
, vol.269
, pp. 6072
-
-
Oh, P.1
-
16
-
-
10244271642
-
-
_ and P. Oh, J. Biol. Chem. 269, 6072 (1994); Am. J. Physiol. 263, H1872 (1992).
-
(1992)
Am. J. Physiol.
, vol.263
-
-
-
17
-
-
0026493761
-
-
J. E. Schnitzer, A. Sung, R. Horvat, J. Bravo, J. Biol. Chem. 264, 24544 (1992); J. E. Schnitzer and J. Bravo, ibid. 268, 7562 (1993).
-
(1992)
J. Biol. Chem.
, vol.264
, pp. 24544
-
-
Schnitzer, J.E.1
Sung, A.2
Horvat, R.3
Bravo, J.4
-
18
-
-
0027478043
-
-
J. E. Schnitzer, A. Sung, R. Horvat, J. Bravo, J. Biol. Chem. 264, 24544 (1992); J. E. Schnitzer and J. Bravo, ibid. 268, 7562 (1993).
-
(1993)
J. Biol. Chem.
, vol.268
, pp. 7562
-
-
Schnitzer, J.E.1
Bravo, J.2
-
19
-
-
0027952583
-
-
E. J. Smart, D. C. Foster, Y.-S. Ying, B. A. Karmen, R. G. W. Anderson, J. Cell Biol. 124, 307 (1994).
-
(1994)
J. Cell Biol.
, vol.124
, pp. 307
-
-
Smart, E.J.1
Foster, D.C.2
Ying, Y.-S.3
Karmen, B.A.4
Anderson, R.G.W.5
-
20
-
-
0024047352
-
-
N. J. Severs, J. Cell Sci. 90, 341 (1988); B. Van Deurs, P. K. Holm, K. Sandvig, S. H. Hansen, Trends Cell Biol. 3, 249 (1993).
-
(1988)
J. Cell Sci.
, vol.90
, pp. 341
-
-
Severs, N.J.1
-
21
-
-
0027216627
-
-
N. J. Severs, J. Cell Sci. 90, 341 (1988); B. Van Deurs, P. K. Holm, K. Sandvig, S. H. Hansen, Trends Cell Biol. 3, 249 (1993).
-
(1993)
Trends Cell Biol.
, vol.3
, pp. 249
-
-
Van Deurs, B.1
Holm, P.K.2
Sandvig, K.3
Hansen, S.H.4
-
22
-
-
0041110797
-
-
M. Bundgaard, J. Frokjaer-Jensen, C. Crone, Proc. Natl. Acad. Sci. U.S.A. 76, 6439 (1979); J. Frokjaer-Jensen, J. Ultrastruct. Res. 73, 9 (1980); M. Bundgaard, Fed. Proc. 42, 2425 (1983); J. Frokjaer-Jensen, R. C. Wagner, S. B. Andrews, P. Hagman, T. S. Reese, Cell Tissue Res. 254, 17 (1988); J. J. Frokjaer-Jensen, J. Electron Microsc. Tech. 19, 291 (1991).
-
(1979)
Proc. Natl. Acad. Sci. U.S.A.
, vol.76
, pp. 6439
-
-
Bundgaard, M.1
Frokjaer-Jensen, J.2
Crone, C.3
-
23
-
-
0019274075
-
-
M. Bundgaard, J. Frokjaer-Jensen, C. Crone, Proc. Natl. Acad. Sci. U.S.A. 76, 6439 (1979); J. Frokjaer-Jensen, J. Ultrastruct. Res. 73, 9 (1980); M. Bundgaard, Fed. Proc. 42, 2425 (1983); J. Frokjaer-Jensen, R. C. Wagner, S. B. Andrews, P. Hagman, T. S. Reese, Cell Tissue Res. 254, 17 (1988); J. J. Frokjaer-Jensen, J. Electron Microsc. Tech. 19, 291 (1991).
-
(1980)
J. Ultrastruct. Res.
, vol.73
, pp. 9
-
-
Frokjaer-Jensen, J.1
-
24
-
-
0021103970
-
-
M. Bundgaard, J. Frokjaer-Jensen, C. Crone, Proc. Natl. Acad. Sci. U.S.A. 76, 6439 (1979); J. Frokjaer-Jensen, J. Ultrastruct. Res. 73, 9 (1980); M. Bundgaard, Fed. Proc. 42, 2425 (1983); J. Frokjaer-Jensen, R. C. Wagner, S. B. Andrews, P. Hagman, T. S. Reese, Cell Tissue Res. 254, 17 (1988); J. J. Frokjaer-Jensen, J. Electron Microsc. Tech. 19, 291 (1991).
-
(1983)
Fed. Proc.
, vol.42
, pp. 2425
-
-
Bundgaard, M.1
-
25
-
-
0024097293
-
-
M. Bundgaard, J. Frokjaer-Jensen, C. Crone, Proc. Natl. Acad. Sci. U.S.A. 76, 6439 (1979); J. Frokjaer-Jensen, J. Ultrastruct. Res. 73, 9 (1980); M. Bundgaard, Fed. Proc. 42, 2425 (1983); J. Frokjaer-Jensen, R. C. Wagner, S. B. Andrews, P. Hagman, T. S. Reese, Cell Tissue Res. 254, 17 (1988); J. J. Frokjaer-Jensen, J. Electron Microsc. Tech. 19, 291 (1991).
-
(1988)
Cell Tissue Res.
, vol.254
, pp. 17
-
-
Frokjaer-Jensen, J.1
Wagner, R.C.2
Andrews, S.B.3
Hagman, P.4
Reese, T.S.5
-
26
-
-
0025996847
-
-
M. Bundgaard, J. Frokjaer-Jensen, C. Crone, Proc. Natl. Acad. Sci. U.S.A. 76, 6439 (1979); J. Frokjaer-Jensen, J. Ultrastruct. Res. 73, 9 (1980); M. Bundgaard, Fed. Proc. 42, 2425 (1983); J. Frokjaer-Jensen, R. C. Wagner, S. B. Andrews, P. Hagman, T. S. Reese, Cell Tissue Res. 254, 17 (1988); J. J. Frokjaer-Jensen, J. Electron Microsc. Tech. 19, 291 (1991).
-
(1991)
J. Electron Microsc. Tech.
, vol.19
, pp. 291
-
-
Frokjaer-Jensen, J.J.1
-
27
-
-
0025303751
-
-
K. G. Rothberg, Y. Ying, J. F. Kolhouse, B. A. Kamen, R. G. W. Anderson, J. Cell Biol. 110, 637 (1990); R. G. W. Anderson, B. A. Kamen, K. G. Rothberg, S. W. Lacey, Science 255, 410 (1992).
-
(1990)
J. Cell Biol.
, vol.110
, pp. 637
-
-
Rothberg, K.G.1
Ying, Y.2
Kolhouse, J.F.3
Kamen, B.A.4
Anderson, R.G.W.5
-
28
-
-
0026545612
-
-
K. G. Rothberg, Y. Ying, J. F. Kolhouse, B. A. Kamen, R. G. W. Anderson, J. Cell Biol. 110, 637 (1990); R. G. W. Anderson, B. A. Kamen, K. G. Rothberg, S. W. Lacey, Science 255, 410 (1992).
-
(1992)
Science
, vol.255
, pp. 410
-
-
Anderson, R.G.W.1
Kamen, B.A.2
Rothberg, K.G.3
Lacey, S.W.4
-
29
-
-
0029082412
-
-
J. E. Schnitzer, D. P. McIntosh, A. M. Dvorak, J. Liu, P. Oh, Science 269, 1435 (1995); J. E. Schnitzer, P. Oh, B. S. Jacobson, A. M. Dvorak, Proc. Natl. Acad. Sci. U.S.A. 92, 1759 (1995).
-
(1995)
Science
, vol.269
, pp. 1435
-
-
Schnitzer, J.E.1
McIntosh, D.P.2
Dvorak, A.M.3
Liu, J.4
Oh, P.5
-
30
-
-
0028925762
-
-
J. E. Schnitzer, D. P. McIntosh, A. M. Dvorak, J. Liu, P. Oh, Science 269, 1435 (1995); J. E. Schnitzer, P. Oh, B. S. Jacobson, A. M. Dvorak, Proc. Natl. Acad. Sci. U.S.A. 92, 1759 (1995).
-
(1995)
Proc. Natl. Acad. Sci. U.S.A.
, vol.92
, pp. 1759
-
-
Schnitzer, J.E.1
Oh, P.2
Jacobson, B.S.3
Dvorak, A.M.4
-
31
-
-
10244272581
-
-
note
-
Cytosol was isolated from rat lungs perfused as described (8, 14). The lungs were flushed free of blood, perfused with cold protease inhibitors, and homogenized in ice-cold cytosolic buffer (2× v/w) (25 mM KCl, 2.5 mM Mg acetate, 5 mM EGTA, 150 mM K acetate, and 25 mM Hepes titrated to a final pH of 7.4 with KOH). The homogenate was centrifuged at 100,000g for 1 hour at 4°C, and the supernatant was collected. This cytosol was filtered with a prepacked DG-10 column (Bio-Rad), and samples were stored frozen at -80°C. Unless indicated otherwise, the filtered cytosol (final concentration, 5 mg/ml) was supplemented just before experimentation with 2 mM ATP and an ATP-regeneration system of creatine phosphokinase (16.7 U/ml) and 16.7 mM phosphocreatine.
-
-
-
-
32
-
-
10244280541
-
-
note
-
A cell-free assay for caveolar fission was reconstituted with the silica-coated luminal endothelial cell plasma membranes purified from rat lungs as described (14). The silica-coated membranes were mixed gently tor 0 to 120 min at 37°C with cytosol (15) containing 1 mM GTP (unless indicated otherwise) before the addition of ice-cold 60% sucrose in 20 mM KCl to achieve a final 30% sucrose concentration. The mixture was layered onto a cushion of 40% sucrose in a SW55 centrifuge tube, and then 20% sucrose was added to the tube before topping with 20 mM KCI. After centrifugation for 2 hours (30,000 rpm in a SW55 rotor) at 4°C, the pellet containing the silicacoated membranes was processed for immunoblot analysis and densitometric quantification of the signal as described (14, 17). For isolation of released caveolae, the membranes were treated as above with 1 mM GTP for 60 min, except that centrifugation was performed with a continuous sucrose gradient as described (15).
-
-
-
-
34
-
-
0028878310
-
-
J. E. Schnitzer, P. Oh, E. Pinney, J. Allard, Am. J. Physiol. 268, H48 (1995);
-
(1995)
Am. J. Physiol.
, vol.268
-
-
Schnitzer, J.E.1
Oh, P.2
Pinney, E.3
Allard, J.4
-
35
-
-
0028294852
-
-
D. Predescu, R. Horvat, S. Predescu, G. E. Palade, Proc. Natl. Acad. Sci. U.S.A. 91, 3014 (1994).
-
(1994)
Proc. Natl. Acad. Sci. U.S.A.
, vol.91
, pp. 3014
-
-
Predescu, D.1
Horvat, R.2
Predescu, S.3
Palade, G.E.4
-
37
-
-
0027970448
-
-
A. M. Fra, E. Williamson, K. Simons, R. G. Parton J. Biol. Chem. 269, 30745 (1994); A. Gorodinsky and D. A. Harris J. Cell Biol. 129, 619 (1995).
-
(1994)
J. Biol. Chem.
, vol.269
, pp. 30745
-
-
Fra, A.M.1
Williamson, E.2
Simons, K.3
Parton, R.G.4
-
38
-
-
0028926204
-
-
A. M. Fra, E. Williamson, K. Simons, R. G. Parton J. Biol. Chem. 269, 30745 (1994); A. Gorodinsky and D. A. Harris J. Cell Biol. 129, 619 (1995).
-
(1995)
J. Cell Biol.
, vol.129
, pp. 619
-
-
Gorodinsky, A.1
Harris, D.A.2
-
40
-
-
10244276521
-
-
The purified silica-coated plasma membranes did not contain detectable ε-COP or other intracellular organelte markers (14, 19)
-
The purified silica-coated plasma membranes did not contain detectable ε-COP or other intracellular organelte markers (14, 19).
-
-
-
-
41
-
-
0026640940
-
-
T. V. Kurzchalia et al., J. Cell Biol. 118, 1003 (1992); P. Dupree, R. G. Parton, G. Raposo, T. V. Kurchalia, K. Simons, EMBO J. 12, 1597 (1993); D. Brown and J. K. Rose, Cell 68, 533 (1992).
-
(1992)
J. Cell Biol.
, vol.118
, pp. 1003
-
-
Kurzchalia, T.V.1
-
42
-
-
0027414646
-
-
T. V. Kurzchalia et al., J. Cell Biol. 118, 1003 (1992); P. Dupree, R. G. Parton, G. Raposo, T. V. Kurchalia, K. Simons, EMBO J. 12, 1597 (1993); D. Brown and J. K. Rose, Cell 68, 533 (1992).
-
(1993)
EMBO J.
, vol.12
, pp. 1597
-
-
Dupree, P.1
Parton, R.G.2
Raposo, G.3
Kurchalia, T.V.4
Simons, K.5
-
43
-
-
0026512314
-
-
T. V. Kurzchalia et al., J. Cell Biol. 118, 1003 (1992); P. Dupree, R. G. Parton, G. Raposo, T. V. Kurchalia, K. Simons, EMBO J. 12, 1597 (1993); D. Brown and J. K. Rose, Cell 68, 533 (1992).
-
(1992)
Cell
, vol.68
, pp. 533
-
-
Brown, D.1
Rose, J.K.2
-
44
-
-
10244280860
-
-
note
-
2, 11 mM glucose (pH 7.4)]. Reduced SLO dissolved in buffer A at 0.6 U/ml was bound at 4°C for 10 min before washing twice in ice-cold buffer A and incubation in buffer A at 37°C for 15 min to induce pore formation in the plasma membranes.
-
-
-
-
45
-
-
0028310326
-
-
J. E. Schnitzer, A. Siflinger-Birnboim, P. J. Del Vecchio, A. B. Malik, Biochem. Biophys. Res. Commun. 199, 11 (1994).
-
(1994)
Biochem. Biophys. Res. Commun.
, vol.199
, pp. 11
-
-
Schnitzer, J.E.1
Siflinger-Birnboim, A.2
Del Vecchio, P.J.3
Malik, A.B.4
-
46
-
-
0025072339
-
-
S. A. Tooze, U. Weiss, W. B. Huttner, Nature 347, 207 (1990); M. F. Rexach and R. W. Schekman, J. Cell Biol. 114, 219 (1991); L. L. Carter, T. E. Redelmeier, L. A. Woolenweber, S. L. Schmid, ibid. 120, 37 (1993).
-
(1990)
Nature
, vol.347
, pp. 207
-
-
Tooze, S.A.1
Weiss, U.2
Huttner, W.B.3
-
47
-
-
0025775567
-
-
S. A. Tooze, U. Weiss, W. B. Huttner, Nature 347, 207 (1990); M. F. Rexach and R. W. Schekman, J. Cell Biol. 114, 219 (1991); L. L. Carter, T. E. Redelmeier, L. A. Woolenweber, S. L. Schmid, ibid. 120, 37 (1993).
-
(1991)
J. Cell Biol.
, vol.114
, pp. 219
-
-
Rexach, M.F.1
Schekman, R.W.2
-
48
-
-
0027498027
-
-
S. A. Tooze, U. Weiss, W. B. Huttner, Nature 347, 207 (1990); M. F. Rexach and R. W. Schekman, J. Cell Biol. 114, 219 (1991); L. L. Carter, T. E. Redelmeier, L. A. Woolenweber, S. L. Schmid, ibid. 120, 37 (1993).
-
(1993)
J. Cell Biol.
, vol.120
, pp. 37
-
-
Carter, L.L.1
Redelmeier, T.E.2
Woolenweber, L.A.3
Schmid, S.L.4
-
49
-
-
10244232372
-
-
V. Maholtra, T. Serafini, L. Orel, J. C. Shepherd, J. E. Rothman, Cell 58, 329 (1939); R. Schwaninger, H. Plutner, G. M. Bokoch, W. E. Balch, J. Cell Biol. 119, 1077 (1992).
-
(1939)
Cell
, vol.58
, pp. 329
-
-
Maholtra, V.1
Serafini, T.2
Orel, L.3
Shepherd, J.C.4
Rothman, J.E.5
-
50
-
-
0026470765
-
-
V. Maholtra, T. Serafini, L. Orel, J. C. Shepherd, J. E. Rothman, Cell 58, 329 (1939); R. Schwaninger, H. Plutner, G. M. Bokoch, W. E. Balch, J. Cell Biol. 119, 1077 (1992).
-
(1992)
J. Cell Biol.
, vol.119
, pp. 1077
-
-
Schwaninger, R.1
Plutner, H.2
Bokoch, G.M.3
Balch, W.E.4
-
51
-
-
0028820041
-
-
E. J. Smart, Y.-S. Ying, C. Mineo, R. W. G. Anderson, Proc. Natl. Acad. Sci. U.S.A. 92, 10104 (1995).
-
(1995)
Proc. Natl. Acad. Sci. U.S.A.
, vol.92
, pp. 10104
-
-
Smart, E.J.1
Ying, Y.-S.2
Mineo, C.3
Anderson, R.W.G.4
-
53
-
-
10244273733
-
-
note
-
We are indebted to R. Jahn, R. A. Skidgel, M. Krieger, and J. P. Luzio for supplying antibodies to VAMP, ACE, ε-COP, and 5'NT, respectively. Supported by the Beth Israel Hospital Foundation, a Grant-in-Aid from the American Heart Association, and NIH grants HL43278 and HL52766 (J.E.S.). Work for this study was carried out during the tenure of an Established Investigatorship Award from the American Heart Association and Genentech (J.E.S.).
-
-
-
|