TrbK, a small cytoplasmic membrane lipoprotein, functions in entry exclusion of the IncPα plasmid RP4

Journal of Bacteriology

Volumn 178, Issue 23, 1996, Pages 6720-6729

  Haase, Jana ^a   Kalkum, Markus ^a   Lanka, Erich ^a  

^a MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GENE PRODUCT; LIPOPROTEIN; MEMBRANE PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL MEMBRANE; BACTERIUM CONJUGATION; ESCHERICHIA COLI K 12; HOST CELL; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN LOCALIZATION; PROTEIN PROCESSING;

BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI K12;

EID: 0029808684     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.23.6720-6729.1996     Document Type: Article

References (80)

1. Achtman, M., N. Kennedy, and R. Skurray. 1977. Cell-cell interaction in conjugating Escherichia cou: role of Ira T protein in surface exclusion. Proc. Natl. Acad. Sei. USA 74:5104-5108.c
2. Achtman, M., A. P. Manning, B. Kusecek, S. Schwuchow, and N. Willens. 1980. A genetic analysis of F sex factor cistrons needed for surface exclusion in Escherichia coli. J. Mol. Biol. 138:779-795.
3. Achtman, M., P. A. Manning, C. Edelbluth, and P. Herrlich. 1979. Export without protcolylic processing of inner and outer membrane proteins encoded by F sex factor tra cistrons in Escherichia coli minicclls. Proc. Nail. Acad. Sci. USA 76:4837-4841.
4. Alt-Morbe, J., J. L. Stryker, C. Fuqua, P.-L. Li, S. K. Farrand, and S. C. Winans. 1996. The conjugal transfer system of Agmbacteriun twnefaciens octopine-type Ti plasmid is closely related to the transfer system of an IncP plasmid and distantly related to Ti plasmid vir genes. J. Bactcriol. 178:42484257.
5. Bachmann, B. J. 1972. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol. Rev. 36:525-557.
6. Beavis, R. C., T. Chaudhary, and B. T. Chait 1992. a-Cyano-4-hydroxycinnamic acid as a matrix for matrix-assisted laser desorption mass spectrometry. Org. Mass. Spectrom. 27:156-158.
7. Bayer, H. W., and D. Roulland-Dussoix. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:459-72.
8. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
9. Bullock, W. O., J. M. Fernandez, and J. M. Short. 1987. XL-1 Blue: a high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. BioTechniques 5:376-378.
10. Chatfield, L. K., E. Orr, G. J. Boulnols, and B. M. Wilkins. 1982. DNA primase of plasrnid Collb is involved in conjugal DNA synthesis in donor and recipient bacteria. J. Bacteriol. 152:1188-1195.
11. Covarrubias, L., and F. Bolivar. 1982. Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivate of pBR328, lacking the 482-hase-pair inverted duplication. Gene 17:79-89.
12. Cowan, P., and V. Krishnapillai. 1982. Tn7 insertion mutations affecting the host range of promiscuous IncP-l plasmid R18. Plasmid 8:164-174.
13. Datta, N., R. VV. Hedges, E. J. Shaw, R. B. Sykes, and M. H. Richmond. 1971. Properties of an R factor from Pseudomonas aeniginosa. J. Bacteriol. 108: 1244-1249.
14. de Maagd, R. A., and B. Lugtenberg. 1986. Fractionation of Rhizobium leguminosantm cells into outer membrane, cytoplasmic membrane, periplasmic, and cytoplasmic components. J. Bacteriol. 167:1083-1085.
15. Derbyshire, K. M., G. Hatfull, and N. Willelts. 1987. Mobilization of the nonconjugative plasmid RSF1010: a genetic and DNA sequence analysis of the mobilization region. Mol. Gen. Genet. 206:161-168.
16. Dev, I. K., and P. H. Ray. 1984. Rapid assay and purification of a unique signal peptidase that processes the prolipoprotein from Escherichia coli. J. Biol. Chem. 259:11114-11120.
17. Devereux, J., P. Haebcrli. and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
18. Dunny, G. M., D. L. Zimmerman, and M. L. Tortorello. 1985. Induction of surface exclusion (entry exclusion) by Streptococcus faecalis sex pheromones: use of monoclonal antibodies to identify an inducible surface antigen involved in the exclusion process. Proc. Nail. Acad. Sci. USA 82:8582-8586.
19. Purste, J. P., W. Pansegrau, R. Frank, H. Blöcker, P. Scholz, M. Bagdasarian, and E. Lanka. 1986. Molecular cloning of the plasrnid RP4 primase region in a multi-host-range tacP expression vector. Gene 48:119-131.
20. Furuya, N., and T. Komano. 1994. Surface exclusion gene of IncI, plasmid R64: nucleotide sequence and analysis of deletion mutants. Plasmid 32:8084.
21. Gennity, J. M., and M. Inouye. 1991. The protein sequence responsible for lipoprotein membrane localization in Escherichia coli exhibits remarkable specificity. J. Biol. Chem. 266:16458-16464.
22. Haase, J.. R. Lurz, M. A. Grahn, D. H. Bamford, and E. Lanka. 1995 Bacterial conjugation mediated by plasmid RP4: RSF1010 mobilization, donor-specific phage propagation, and pilus production require the same Tra2 core components of a proposed DNA transport complex. J. Bacteriol. 177:4779-4791.
23. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
24. Hartskeerl, R., P. Overduin, W. Hoekstra, and J. Tommassen. 1986. Nucleotide sequence of the exclusion-determining locus of the Incl plasmid R144. Gene 42:107-111.
25. Hartskeerl, R., J. Tommassen, and W. Hoekstra. 1985. Relationship between the proteins encoded by the exclusion determining locus of the Incl plasmid R144 and the cellular localization of these proteins in Escherichia coli K-12. Mol. Gen. Genet. 200:138-144.
26. Hartskeerl, R., E. Zuidweg, M. van Geffen, and W. Hoekstra. 1985. The Incl plasmids R144, R64 and Collb belong to one exclusion group. J. Gen. Microbiol. 131:1305-1311.
27. Hartskeerl, R. A., J. E. N. Bergmans, M. C. Kamp, and W. P. M. Hoekstra. 1983. Cloning of an exclusion-determining fragment of the Incl plasmid, R144. Plasmid 10:11-20.
28. Hartskeerl, R. A., and W. P. M. Hoekstra. 1984. Exclusion in Incl-type Escherichia coli conjugation: the stage of conjugation at which exclusion operates. Antonie Leeuwenhoek J. Microbiol. Serol. 50:113-124.
29. Hedges, R. W., and A. E. Jacob. 1974. Transposition of ampicillin resistance from RP4 to other replicons. Mol. Gen. Genet. 132:31-40.
30. Hjerten, S., and R. Mosbach. 1962. "Molecular-sieve" chromatography of proteins on columns of cross-linked polyacrylamide. Anal. Biochem. 3:109118.
31. Howland, C. J., C. E. D. Rees, P. T. Barth, and B. M. Wilkins. 1989. The ssb gene of plasmid ColIb-P9. J. Bacteriol. 171:2466-2473.
32. Hussain. M., S. Ichihara, and S. Mizushima. 1980. Accumulation of glyceride-containing precursor of the outer membrane lipoprotein in the cytoplasmic membrane of Escherichia coli treated with globomycin. J. Biol. Chem. 255:3707-3712.
33. Jalajakumari, A. G., H. J. Bunk, and P. A. Manning. 1987. Surface exclusion genes tra S and tr aT of the F sex factor of Escherichia coli K-12. J. Mol. Biol. 198:1-11.
34. Jobanputra, R. S., and N. Datta. 1974. Trimethopnm R factors in enterobacteria from clinical specimens. J. Med. Microbiol. 7:169-177.
35. Kao, S.-M., S. B. Olmsted. A. S. Viksnins, J. C. Gallo, and G. M. Dunny. 1991. Molecular and genetic analysis of a region of plasmid pCFlO containing positive control genes and structural genes encoding surface proteins involved in pheromone-induciblc conjugation in Ewerococcus faecalis. J. Bacteriol. 173:7650-7664.
36. Karas, M., and F. Hillenkamp. 1988. Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Anal. Chem. 60:2299-2301.
37. LaVallie, E. R., E. A. DiBlasio. S. Kovacic, K. L. Granl, P. F. Schendel, and J. M. McCoy. 1993. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio/Technology 11:187-193.
38. Lederberg, J., L. Cavalli, and E. M. Lederberg. 1952. Sex compatibility in Escherichia coli. Genetics 37:720-730.
39. Lessl, M., D. Balzer, R. Lurz, V. L. Waters. D. G. Guiney, and E. Lanka. 1992. Dissection of IncP conjugative plasmid transfer: definition of the transfer region Tra2 by mobilization of the Tral region in trans. J. Bacteriol. 174:2493-2500.
40. Lessl, M., D. Balzer, W. Pansegrau, and E. Lanka. 1992. Sequence similarities between the RP4 Tra2 and the Ti VirB region strongly support the conjugation model for T-DNA transfer. J. Biol. Chem. 267:20471-20480.
41. Lessl, M., D. Balzer, K. Weyrauch, and E. Lanka. 1993. The mating pair formation system of plasmid RP4 defined by RSF1010 mobilization and donor-specific phage propagation. J. Bacleriol. 175:6425-6425.
42. Lessl, M., V. Krishnapillai, and W. Schilf. 1991. Identification and characterization of two entry exclusion genes of the promiscuous IncP plasmid R18. Mol. Gen. Genet. 227:120-126.
43. Lu, M. J., and U. Henning. 1994. Superinfection exclusion by T-even-type coliphages. Trends Microbiol. 2:137-139.
44. Lyras, D., A. W. S. Chan, J. Mcfarlane, and V. A. Stanisich. 1994. The surface exclusion system of RP1 : investigation of the roles of trbJ and trbK in the surface exclusion, transfer, and slow-growth phenotypes. Plasmid 32:254261.
45. Messing, J., and J. Vieira. 1982. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269-276.
46. Miele, L., B. Strack, V. Kmft, and E. Lanka. 1991. Gene organization and nucleotide sequence of the primase region of IncP plasmids RP4 and R751. DNA Seq. 2:145-162.
47. Miller, J. H. 1972. Experiments in molecular genetics, p. 431-433. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
48. Novick, R. P. 1969. Extrachromosomal inheritance in bacteria. Bacteriol. Rev. 33:210-235.
49. Olmsted, S. B., S. M. Kao, L. J. ran Putte, J. C. Gallo, and G. M. Dunny. 1991. Role of the pheromone-inducible surface protein Asc 10 in mating aggregate formation and conjugal transfer of the Enlemcoccus faecalis plasmid pCFlO. J. Bacteriol. 173:7665-7672.
50. Osborn, M. J., J. E. Gander, E. Parisi, and J. Carson. 1972. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J. Biol. Chem. 247: 3962-3972.
51. Pansegrau, W., D. Balzer, V. Kruft, R. Lurz, and E. Lanka. 1990. in vitro assembly of relaxosomes at the transfer origin of plasmid RP4. Proc. Natl. Acad. Sci. USA 87:6555-6559.
52. Pansegrau, W., E. Lanka, P. T. Barth, D. H. Figurski, D. G. Guiney, D. Haas, D. R. Helinski, H. Schwab, V. A. SUnisich, and C. M. Thomas. 1994. Complete nucleotide sequence of Birmingham IncPaα plasmids: compilation and comparative analysis. J. Mol. Biol. 239:623-663.
53. Pappin, D. J. C., H. F. Rahman, H. F. Hansen, M. Bartlet-Jones, W. Jeffery, and A. J. Bleasby. 1995. Chemistry, mass spectrometry and peptide-mass databases: evolution of methods for the rapid identification and mapping of cellular proteins. In A. L. Burlingame and S. A. Carr (ed.), Proceedings of the Third International Symposium on Mass Spectrometry in Health and Life Science. Humana Press, Clifton, N.J.
54. Perumal, N. B., and J. E. G. Minkley. 1984. The product of the F sex factor tra T surface exclusion gene is a lipoprotein. J. Biol. Chem. 259:5357-5360.
55. Pohlman,R. F., H. D. Genetti, and S. C. Winans. 1994. Entry exclusion of the IncN plasmid-pKM 101 is mediated by a single hydrophilic protein containing a lipid attachment motif. Plasmid 31:158-165.
56. Rees, C. E. D., D. E. Bradley, and B. M. Wilkins. 1987. Organization and regulation of the conjugation genes of IncI, plasmid Collb-P9. Plasmid 18:223-236.
57. Riede, L, and M.-L. Eschbach. 1986. Evidence that TraT interacts with OmpA of Escherichia coli. FEES Lett. 205:241-245.
58. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
59. Sankaran, K., and H. C. Wu. 1994. Lipid modification of bacterial prolipoproteins. J. Biol. Chem. 269:19701-19706.
60. Sayers, J. R., W. Schmidt, and F. Eckstein. 1988. 5′ -3′ exonucleases in phosphorothioate-based oligonucleotide-dirccted mutagenesis. Nucleic Acids Res. 16:791-802.
61. Schägger, H., and G. V. Jagow. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophorcsis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochcm. 166:368-379.
62. Schnaitman, C. A. 1970. Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis. J. Bacteriol. 104:882-889.
63. - mutants: class of mutants in Escherichia coli K-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage. J. Bacteriol. 119:726-735.
64. Strack, B., M. Lessl, R. Calendar, and E. Lanka. 1992. A common sequence motif, -E-G-Y-A-T-A-, identified within the primase domains of plasmidencoded I- and P-type DNA primases and the a protein of the Escherichia coli satellite phage P4. J. Biol. Chem. 267:13062-13072.
65. Sukupolvi. S., and D. O'Connor. 1990. TraT lipoprotcin, a plasmid-specified mediator of interactions between gram-negative bacteria and their environment. Microbiol. Rev. 54:331-341.
66. Tanaka, K., H. Waki, Y. Ido, S. Akita, Y. Yoshida, and T. Yoshida. 1988. Protein and polymer analysis up to m/z 100,000 by laser time-of-flight mass spectrometry. Mass. Spectrom. 2:151-153.
67. Thorsted, P. B., D. Macartney, P. Aklar, IS. AH, P. Davidson, T. Stafford, C. Thelwell, E. Lanka, and C. M. Thomas. 1996. Complete sequence of the IncPβ plasmid R751: comparison with the Birmingham IncPcr plasmids. European Science Foundation Network Workshop on Molecular Biology and Ecology of Plasmid-Mediated Gene Transfer. Potsdam, Germany.
68. Tokunaga, M., H. Tokunaga, and H. C. Wu. 1982. Post-translational modification and processing of Escherichia coli prolipoprotein in vitro. Proc. Natl. Acad. Sci. USA 79:2255-2259.
69. Towbin, H., T. Staebelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
70. Vestal, M. L., P. Juhasz, and S. A. Martin. 1995. Delayed extraction matrixassisted laser desorption time-of-flight mass spectrometry. Mass Spectrom. 9:1044-1050.
71. von Heijne, G. 1990. The signal peptide. J. Membr. Biol. 115:195-201.
72. Waters, V. L., B. Strack, W. Pansegrau, E. Lanka, and D. G. Guiney. 1992. Mutational analysis of essential IncPβ plasmid transfer genes traf and traG and involvement of traf in phage sensitivity. J. Bacteriol. 174:6666-6673.
73. Weidlich, G., R. Wirth, and D. Galli. 1992. Sex pheromone plasmid pADlencoded surface exclusion protein oiEnterococcusfaecalis. Mol. Gen. Genet. 233:161-168.
74. Willetts, N., and J. Maule. 1974. Interactions between the surface exclusion systems of some F-like plasmids. Genet. Res. 24:81-89.
75. Winans, S. C., and G. C. Walker. 1985. Conjugal transfer of the IncN plasmid pKMIOl. J. Bacteriol. 161:402-410.
76. Winans, S. C., and G. C. Walker. 1985. Entry exclusion determinant(s) of the IncN plasmid pKM101. J. Bacteriol. 161:411-416.
77. Yamada, Y., M. Yamada, and A. Kakazawa. 1995. A ColEl-encoded gene directs entry exclusion of the plasmid. J. Bacteriol. 177:6064-6068.
78. Yamaguchi, K., F. Yu, and M. Inouye. 1988. A single amino acid determinant of the memhrane localization of lipoproteins in E. coli. Cell 53:423-432.
79. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103-119.
80. Ziegelin, G., W. Pansegrau, B. Strack, D. Balzer, M. Kroger, V. Kruft, and E. Lanka. 1991. Nucleotide sequence and organization of genes flanking the transfer origin of promiscuous plasmid RP4. DNA Seq. 1:303-327.