Evaluation of methods for preparing and thawing cryopreserved CD34+ and CD34- cell lines for use as reagents in flow cytometry of hematopoietic progenitor cells

Transfusion

Volumn 36, Issue 11-12, 1996, Pages 985-988

  Bowman, C A ^a   Yu, M ^a   Cottler Fox, M ^a,b  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD34 ANTIGEN; FICOLL; REAGENT;

ANTIGEN EXPRESSION; ARTICLE; BLOOD COMPONENT; CELL STRAIN K 562; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LEUKEMIA CELL LINE; QUALITY CONTROL; THAWING;

EID: 0029807783     PISSN: 00411132     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1537-2995.1996.36111297091743.x     Document Type: Article

References (8)

1. McCoy JP, Carey JL, Krause JR. Quality control in flow cytometry for diagnostic pathology. Am J Clin Pathol 1990;93(4 suppl 1):S27-37.
2. Lubbert M, Koeffler HP. Myeloid cell lines: tools for studying differentiation of normal and abnormal hematopoietic cells. Blood Rev 1988;2:121-33.
3. Sutherland DR, Watt SM, Dowden G, et al. Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34. Leukemia 1988;2:793-803.
4. Areman EM, Deeg HJ, Sacher RA, eds. Bone marrow and stem cell processing: a manual of current techniques. Philadelphia: F.A. Davis, 1992.
5. Caldwell CW, Maggi J, Henry LB, Taylor HM. Fluorescence intensity as a quality control parameter in clinical flow cytometry. Am J Clin Pathol 1987;88:447-56.
6. Yu M, Cottler-Fox M. Quality assurance in flow cytometry: comparison of three analytical methods for quantifying CD34+ cells in marrow. Prog Clin Biol Res 1994;389:729-36.
7. Henry JB. Clinical diagnosis and management by laboratory methods. 17th ed. Philadelphia: Saunders, 1984.
8. Finney DJ. Statistics for biologists. London: Chapman and Hall, 1980.