Heterogeneity of huma serum cholinesterase revealed by thiocholine substrates

Periodicum Biologorum

Volumn 98, Issue 3, 1996, Pages 331-335

  Simeon Rudolf, Vera ^a   Juršić, Brigita ^a,b  

^a INSTITUTE FOR MEDICAL RESEARCH AND OCCUPATIONAL HEALTH   (Croatia)

^b University of New Orleans   (United States)

Author keywords

Acetylthiocholine; Butyrylthiocholine; Human serum cholinesterase; Inhibition; Molecular forms; PAA electrophoresis; Propionylthiochol

Indexed keywords

ACETYLTHIOCHOLINE; BUTYRYLTHIOCHOLINE; CHOLINESTERASE; CHOLINESTERASE INHIBITOR; THIOCHOLINE;

ARTICLE; CHOLINESTERASE BLOOD LEVEL; ELECTROPHORESIS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GENETIC HETEROGENEITY; HUMAN; MICHAELIS MENTEN KINETICS; MOLECULAR WEIGHT; PHENOTYPE;

EID: 0029806836     PISSN: 00315362     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. AUGUSTINSSON K B, BARTFAI T, MANNERVIK B 1974 A steady-state kinetic model of butyrylcholinesterase from horse plasma. Biochem J 141: 825-834
2. BROCK A 1989 Additional electrophoretic components of cholinesterase in plasma: A phenomenon of no importance to the total plasma cholinesterase activity. J Clin Chem Clin Biochem 27: 429-431
3. CAUET G, FRIBOULET A, THOMAS D 1987 Substrate activation and thermal denaturation kinetics of the tetrameric and trypsin-generated monomeric forms of horse serum butyrycholine-sterase. Biochem Biophys Acta 912: 338-342
4. CAUET G, FRIBOULET A, THOMAS D 1987 Horse serum butyrylcholinesterase kinetics: a molecular mechanism based on inhibition studies with dansylaminoethyltrimethylammonium. Biochem Cell Biol 65. 529-535
5. CHIU Y C, TRIPATHI R K, O'BRIEN R D 1972 Differences in reactivity of four butyrylcholinesterase isozymes towards substrate and inhibitors. Biochem Biophys Res Comm 46: 3542
6. ELLMAN G L, COURTNEY K D, ANDRES V Jr, FEATHERSTONE R M 1961 A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmac 7: 88-95
7. EVANS R T 1986 Cholinesterase phenotyping: Clinical aspects and laboratory applications. CRC Crit Rev Clin Lab Sci 23: 3-64
8. GAFFNEY P J Jr 1970 Human serum cholinesterase I. Partial 1 unification and nature of the heterogeneity of this system. Biochem Biophys Acta 207: 465-476
9. HAREL M, SUSSMAN J L, KREJCI E, BON S, CHANAL P, MASSOULIÉ J, SILMAN I 1992 Conversion of acetylcholinesterase to butyrylcholinesterase: Modeling and mutagenesis. Proc Natl Acad Sci USA 89: 10827-10831
10. HOSEA N A, BERMAN H A, TAYLOR P 1995 Specificity and orientation of trigonal carboxyl esters and tetrahedral alkylphosphonyl esters in cholinesterases. Biochemistry 34: 11528-11536
11. JUUL P 1968 Human plasma cholinesterase isoenzymes. Clin Chim Acta 19: 205-213
12. MASSON P, ADKINS S, GOUET P, LOCKRIDGE O 1993 Recombinant human butyrylcholinesterase G390V, the fluoride-2 variant, expressed in Chinese hamster ovary cells, is a low affinity variant. J Biool Chem 268: 14329-14341
13. LaMOTTA R V, WORONICK CH L, REINFRANK R F 1970 Multiple forms of serum cholinesterase: Molecular weights of the isoenzymes. Arch Biochem Biophys 136: 448-451
14. MUENSCH H, GOEDDE H-W, YOSHIDA A 1973 Human-serum cholinesterase subunits and number of active sites of the major component. Eur J Biochem 70: 217-223
15. NAGY I, SASHEGYI J, KURCZ M, BARANYAI P 1974 Separation of serum cholinesterase isozymes by polyacrilamide gradient gel electrophoresis. Acta Biochem Biophys Acad Sci Hung 9: 209-212
16. ORDENTLICH A, BARAK D, KRONMAN CH, FLASHNER Y, LEITNER M, SEGALL Y, ARIEL N, COHEN S, VELAN B, SHAFFERMAN A 1993 Dissection of the human acetylcholinesterase, active center determinants of substrate specificity. J Biol Chem 268: 17083-17095
17. POLYACRILAMIDE GEL ELECTROPHORESIS 1980 Laboratory techniques Pharmacia Fine Chemicals. Lund, p 1, 8-12
18. REINER E., SIMEON-RUDOLF V, ŠKRINJARIĆ-ŠPOLJAR M 1995 Catalytic properties and distribution profiles of paraoxonase and cholinesterase in human sera. Toxicology Letters 82/83: 447-452
19. SIMEON V, BUNTIĆ A, ŠURINA B, FLEGAR-MEŠTRIĆ Z 1987 Cholinesterase phenotyping and distribution of activity in sera of 346 individuals. Acta Pharm lugosl 37: 107-114
20. SIMEON V, REINER E, ŠKRINJARIĆ-ŠPOLJAR M, KRAUTHACKER B 1988 Cholinesterase in rabbit serum. Gen Pharmac 19: 849-853
21. VELLOM D C, RADIĆ Z, LI Y, PICKERING N A, CAMP S, TAYLOR P 1993 Amino acid residues controlling acetlcholinesterase and butyrylcholinesterase specificity. Biochemistry 32: 12-17