Bacterial plasmolysis as a physical indicator of viability

Applied and Environmental Microbiology

Volumn 62, Issue 11, 1996, Pages 3939-3947

  Korber, D R ^a   Choi, A ^a   Wolfaardt, G M ^a   Caldwell, D E ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

SODIUM CHLORIDE;

ARTICLE; BACTERICIDAL ACTIVITY; BIOFILM; CELL VIABILITY; CONTROLLED STUDY; LIGHT SCATTERING; LYSIS; NONHUMAN; OSMOTIC STRESS; PROTOPLAST; PSEUDOMONAS FLUORESCENS; SALMONELLA ENTERITIDIS;

BACTERIA (MICROORGANISMS); ENTERITIDIS; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; SALMONELLA ENTERITIDIS;

EID: 0029805912     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.11.3939-3947.1996     Document Type: Article

References (42)

1. Atlas, R. M. 1984. Use of microbial diversity measurements to assess environmental stress, p. 540-545. In M. J. Klug and C. A. Reddy (ed.), Current perspectives in microbial ecology. ASM Press, Washington, D.C.
2. Betts, R. P., P. Bankes, and J. G. Banks. 1989. Rapid enumeration of viable micro-organisms by staining and direct microscopy. Lett. Appl. Microbiol. 9:199-202.
3. Bottomley, P. J., and S. P. Maggard. 1990. Determination of viability within serotypes of a soil population of Rhizobium leguminosarum bv. trifolii. Appl. Environ. Microbiol. 56:533-540.
4. Brock, T. D. 1987. The study of microorganisms in situ: progress and problems, p. 1-20. In M. Fletcher, T. R. G. Gray, and J. G. Jones (ed.), Ecology of microbial communities. Cambridge University Press, Cambridge.
5. Caldwell, D. E., D. R. Korber, and J. R. Lawrence. 1992. Imaging of bacterial cells by fluorescence exclusion using scanning confocal laser microscopy. J. Microbiol. Methods 15:249-261.
6. Caldwell, D. E., and J. R. Lawrence. 1986. Growth kinetics of Pseudomonas fluorescens microcolonies within the hydrodynamic boundary layers of surface microenvironments. Microb. Ecol. 12:299-312.
7. Caldwell, D. E., and J. R. Lawrence. 1989. Microbial growth and behavior within surface microenvironments, p. 140-145. In T. Hattori, Y. Ishida, Y. Maruyama, R. Y. Morita, and A. Uchida (ed.), Recent advances in microbial ecology. Japan Scientific Societies Press, Tokyo.
8. Daley, R. J. 1979. Direct epifluorescence enumeration of native aquatic bacteria: uses, limitations, and comparative accuracy, p. 29-45. In J. W. Costerton and R. R. Colwell (ed.), Native aquatic bacteria: enumeration, activity, and ecology. American Society for Testing and Materials publication no. 695.
9. Dhavises, G., and G. D. Anagnostopoulos. 1979. Influence of amino acids and water activity on the growth of Escherichia coli B/r/1. Microbios Lett. 7:105-115.
10. Fischer, A. 1900. In A. C. Jones (ed.), The structure and function of bacteria, p. 4-9. Oxford Press, Oxford.
11. Geesey, G. G., and R. Y. Morita. 1981. Relationship of cell envelope stability to substrate capture in a marine psychrophilic bacterium. Appl. Environ. Microbiol. 42:533-540.
12. Hua, S.-S. T., V. Y. Tsa, G. M. Lichens, and A. T. Noma. 1982. Accumulation of amino acids in Rhizobium sp. strain WR1001 in response to sodium chloride salinity. Appl. Environ. Microbiol. 44:135-140.
13. Jensen, V. 1968. The plate count technique, p. 158-170. In T. R. G. Gray and D. Parkinson (ed.), The ecology of soil bacteria. University of Toronto Press, Toronto.
14. Johnson, F. H., and E. N. Harvey. 1937. The osmotic and surface properties of marine luminous bacteria. J. Cell. Comp. Physiol. 9:363-380.
15. Kaprelyants, A. S., and D. B. Kell. 1992. Rapid assessment of bacterial viability and vitality by rhodamine 123 and flow cytometry. J. Appl. Bacteriol. 72:410-422.
16. Killham, K., and M. K. Firestone. 1984. Salt stress control of intracellular solutes in streptomycetes indigenous to saline soils. Appl. Environ. Microbiol. 47:301-306.
17. Koch, A. L. 1984. Shrinkage of growing Escherichia coli cells by osmotic challenge. J. Bacteriol. 159:919-924.
18. Korber, D. R., G. A. James, and J. W. Costerton. 1994. Evaluation of fleroxacin activity against established Pseudomonas fluorescens biofilms. Appl. Environ. Microbiol. 60:1663-1669.
19. Korber, D. R., J. R. Lawrence, K. E. Cooksey, B. Cooksey, and D. E. Caldwell. 1989. Computer image analysis of diatom chemotaxis. Binary: Comput. Microbiol. 1:155-168.
20. - Pseudomonas fluorescens biofilms using representative elements. Biofouling 7:339-358.
21. Korber, D. R., J. R. Lawrence, H. M. Lappin-Scott, and J. W. Costerton. 1995. Growth of microorganisms on surfaces, p. 15-45. In H. M. Lappin-Scott and J. W. Costerton (ed.), Microbial biofilms. Cambridge University Press, Cambridge.
22. - Pseudomonas fluorescens. Microb. Ecol. 18:1-19.
23. Kurath, G., and R. Y. Morita. 1983. Starvation-survival physiological studies of a marine Pseudomonas sp. Appl. Environ. Microbiol. 45:1206-1211.
24. Lawrence, J. R., D. R. Korber, and D. E. Caldwell. 1989. Computer-enhanced darkfield microscopy for the quantitative analysis of bacterial growth and behavior on surfaces. J. Microbiol. Methods 10:123-138.
25. Lawrence, J. R., D. R. Korber, and D. E. Caldwell. 1992. Behavioral analysis of Vibrio parahaemolyticus variants in high- and low-viscosity microenvironments using computer-enhanced microscopy. J. Bacteriol. 174:5732-5739.
26. Mager, J., M. Kuckzynski, G. Schatzberg, and Y. Avi-Dor. 1956. Turbidity changes in bacterial suspensions in relation to osmotic pressure. J. Gen. Microbiol. 14:69-76.
27. Marquis, R. E. 1968. Salt-induced contraction of bacterial cell walls. J. Bacteriol. 95:775-781.
28. Mitchell, P., and J. Moyle. 1956. Osmotic function and structure in bacteria. Symp. Soc. Gen. Microbiol. 6:150-180
29. Morita, R. Y. 1985. Starvation and miniaturisation of heterotrophs with special emphasis on maintenance of starved viable state, p. 111-130. In M. Fletcher and G. D. Floodgate (ed.), Bacteria in their natural environments. Academic Press, Inc., London.
30. Nix, P. G., and M. M. Daykin. 1992. Resazurin reduction tests as an estimate of coliform and heterotrophic bacterial numbers in environmental samples. Bull. Environ. Contam. Toxicol. 49:354-360.
31. Novitsky, T. J. 1984. Monitoring and validation of high purity water systems with the Limulus amebocyte lysate test for pyrogens. Pharm. Eng. 4:21-33.
32. Postgate, J. 1989. A microbial way of death. New Sci. 20:43-47.
33. Postgate, J. R., J. E. Crumpton, and J. R. Hunter. 1961. The measurement of bacterial viabilities by slide culture. J. Gen. Microbiol. 24:15-24.
34. Rodriguez, G. G., D. Phipps, K. Ishiguro, and H. F. Ridgway. 1992. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58:1801-1808.
35. Roszak, D. B., and R. R. Collwell. 1987. Survival strategies of bacteria in the natural environment. Microbiol. Rev. 51:365-379.
36. Schall, B. F., G. V. Marathe, and B. K. Ghosh. 1981. Stereological analysis of plasmolysis in logarithmic-phase Bacillus licheniformis. J. Bacteriol. 146: 391-397.
37. Scheie, P. O. 1969. Plasmolysis of Escherichia coli B/r with sucrose. J. Bacteriol. 98:335-340.
38. Sperber, W. H. 1983. Influence of water activity on foodborne bacteria - a review. J. Food Prot. 46:142-150.
39. Stubberfield, L. C. F., and P. J. A. Shaw. 1990. A comparison of tetrazolium reduction and FDA hydrolysis with other measures of microbial activity. J. Microbiol. Methods 12:151-162.
40. Tabor, P. S., and R. A. Neihof. 1982. Improved method for determination of respiring individual microorganisms in natural waters. Appl. Environ. Microbiol. 43:1249-1255.
41. Ward, D. M., R. Weller, and M. M. Bateson. 1990. 16S rRNA sequences reveal uncultured inhabitants of a well-studied thermal community. Nature (London) 345:63-65.
42. Yu, F. P., and G. A. McFeters. 1994. Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes. J. Microbiol. Methods 20:1-10.