Activity and thermostability of the small self-splicing group I intron in the pre-tRNA(Ile) of the purple bacterium Azoarcus

RNA

Volumn 2, Issue 1, 1996, Pages 74-83

  Tanner, Michael A ^a   Cech, Thomas R ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

Anabaena; Azoarcus; GAAA loop; group I intron; melting curves; self splicing activity

Indexed keywords

BACTERIAL RNA; RNA PRECURSOR; TRANSFER RNA;

ARTICLE; BACTERIUM; CONTROLLED STUDY; INTRON; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY; RNA SEQUENCE; RNA SPLICING; THERMOSTABILITY;

BASE SEQUENCE; GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS; INTRONS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RNA PRECURSORS; RNA SPLICING; RNA, TRANSFER, ILE; TEMPERATURE;

ANABAENA; AZOARCUS; AZOARCUS SP.; BACTERIA (MICROORGANISMS); FELIS CATUS;

EID: 0029805902     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Angata K, Ogawa S, Yanagisawa K, Tanaka Y. 1995. A group-I intron in the mitochondrial large-subunit ribosomal RNA-encoding gene of Dictyostelium discoideum: Same site localization in alga and in vitro self-splicing. Gene 153:49-55.
2. Banerjee AR, Jaeger JA, Turner DH. 1993. Thermal unfolding of a group I ribozyme: The low-temperature transition is primarily disruption of tertiary structure. Biochemistry 32:153-163.
3. Barfod ET, Cech TR. 1988. Deletion of nonconserved helices near the 3′ end of the rRNA intron of Tetrahymena thermophila alters self-splicing but not core catalytic activity. Genes & Dev 2:652-663.
4. Beaudry AA, Joyce GF. 1990. Minimum secondary structure requirements for catalytic activity of a self-splicing group I intron. Biochemistry 29:6534-6539.
5. Biniszkiewicz D, Cesnaviciene E, Shub DA. 1994. Self-splicing group I intron in cyanobacterial initiator methionine tRNA: Evidence for lateral transfer of introns in bacteria. EMBO J 13:4629-4635.
6. Burke JM, Irvine KD, Kaneko JK, Kerker BJ, Oettgen AB, Tierney WM, Williamson CL, Zaug AJ, Cech TR. 1986. Role of conserved sequence elements 9L and 2 in self-splicing of the Tetrahymena ribosomal RNA precursor. Cell 45:167-176.
7. Cech TR. 1990. Self-splicing of group I introns. Annu Rev Biochem 59:543-568.
8. Cech TR. 1993. Structure and mechanism of the large catalytic RNAs: Group I and group II introns and ribonuclease P. In: Gesteland RF, Atkins JF, eds. The RNA world. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, pp 239-269.
9. Cech TR, Bass BL. 1986. Biological catalysis by RNA. Annu Rev Biochem 55:599-629.
10. Cech TR, Damberger SH, Gutell RR. 1994. Representation of the secondary and tertiary structure of group I introns. Nature Struct Biol 1:273-280.
11. Cech TR, Herschlag D, Piccirilli JA, Pyle AM. 1992. RNA catalysis by a group I ribozyme. J Biol Chem 267:17479-17482.
12. Cech TR, Zaug AJ, Grabowski PJ. 1981. In vitro splicing of the ribosomal RNA precursor of Tetrahymena: Involvement of a guanosine nucleotide in the excision of the intervening sequence. Cell 27:487-496.
13. Chu FK, Maley GF, Maley F. 1987. Mechanism and requirements of in vitro RNA splicing of the primary transcript from the T4 bacteriophage thymidylate synthase gene. Biochemistry 26:3050-3057.
14. Costa M, Michel F. 1995. Frequent use of the same tertiary motif by self-folding RNAs. EMBO J 14:1276-1285.
15. Damberger SH, Gutell RR. 1994. A comparative database of group I intron structures. Nucleic Acids Res 22:3508-3510.
16. Davanloo P, Rosenberg AH, Dunn JJ, Studier FW. 1984. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc Natl Acad Sci USA 81:2035-2039.
17. Deng WP, Nickoloff JA. 1992. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal Biochem 200:81-88.
18. Doudna JA, Grosshans C, Gooding A, Kundrot CE. 1993. Crystallization of ribozymes and small RNA motifs by a sparse matrix approach. Proc Natl Acad Sci USA 90:7829-7833.
19. Ehrenman K, Pedersen-Lane J, West D, Herman R, Maley F, Belfort M. 1986. Processing of phage T4 td-encoded RNA is analogous to the eukaryotic group I splicing pathway. Proc Natl Acad Sci USA 83:5875-5879.