Cell culture observations of human postnatal thymic epithelium: An in vitro model for growth and humoral influence on intrathymic T lymphocyte maturation

In Vivo

Volumn 10, Issue 5, 1996, Pages 515-526

  Bodey, Bela ^a,d   Bodey Jr , Bela ^a   Kaiser, Hans E ^b,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF VIENNA   (Austria)

^d UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

FACS analysis; Immunocytochemistry; Immunophenotype (IP); Interleukin 2 receptor; Lymphocyte differentiation antigens; Monoclonal antibodies (MoABs); Postnatal development; Proliferation assay; Recombinant interleukin 2; Reticulo epithelial cell; Scanning electronmicroscopy (SEM); T lymphocytes; Thymic accessory cells; Thymic cell microenvironment (TCM); Thymocytes; Transmission electronmicroscopy (TEM)

Indexed keywords

CD11 ANTIGEN; CD3 ANTIGEN; CD7 ANTIGEN; CYTOKERATIN; CYTOSKELETON PROTEIN; INTERLEUKIN 2 RECEPTOR; LYMPHOCYTE ANTIGEN; MITOGENIC AGENT; MONOCLONAL ANTIBODY; PHYTOHEMAGGLUTININ; RECOMBINANT INTERLEUKIN 2; TRITIUM; VIMENTIN;

ARTICLE; CELL CULTURE; CELL ISOLATION; CELL PROLIFERATION; CHILD; CLINICAL ARTICLE; EPITHELIUM CELL; FLUORESCENCE ACTIVATED CELL SORTER; HELPER CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; IMMUNOPHENOTYPING; INFANT; ISOTOPE LABELING; LYMPHOCYTE DIFFERENTIATION; RECEPTOR UPREGULATION; SCHOOL CHILD; T LYMPHOCYTE; THYMOCYTE; THYMUS; TONOFILAMENT;

EID: 0029804328     PISSN: 0258851X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (88)

1. Reinherz EL, Kung PC, Goldstein G, Levey RH and Schlossman SF: Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci USA 77: 1588-1592, 1980.
2. Reinherz EL and Schlossman SF: The differentiation and function of human T lymphocytes. Cell 19: 821-827, 1980.
3. Janossy G, Tidman N, Papageorgiou ES, Kung PC and Goldstein G: Distribution of T lymphocyte subsets in the human bone marrow and thymus: an analysis with monoclonal antibodies. J Immunol, 126: 1608-1613, 1981.
4. Campana D, Janossy G, Coustan-Smith E, Amlot PL, Tian WT, Ip S and Wong L: The expression of T cell receptor-associated proteins during T cell ontogeny in man. J Immunol 142: 57-66, 1989.
5. Haynes BF: The human thymic microenvironment In: Advances in Immunology, FJ Dixon ed. 36: 87-142, 1984.
6. Hershfield MS, Kurtzberg J, Harden E, Moore JO, Whang-Peng J and Haynes BF: Conversion of a stem cell leukemia from a T-lymphoid to a myeloid phenotype induced by the adenosine deaminase inhibitor 2'-deoxycoformycin. Proc Natl Acad Sci USA 81: 253-257, 1984.
7. Kurtzberg J, Bigner SH and Hershfield MS: Establishment of the DU. 528 human lymphohemopoietic stem cell line. J Exp Med 162: 1561-1578, 1985.
8. Medlock ES and Goldschneider I: Generation of terminal deoxynucleotidyltransferase-positive lymphoid precursor cells in vitro. Methods Enzymol 150: 363-374, 1987.
9. Picker LJ, De los Toyos J, Telen MJ, Haynes BF and Butcher EC: Monoclonal antibodies against the CD44 In(Lu)-related p80, and Pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors. J Immunol 142: 2046-2051, 1989.
10. Haynes BF, Grover BJ, Whichard LP, Hale LP, Nunley JA, McCollum DE and Singer KH: Synovial microenvironment-T cell interactions. Human T cells bind to fibroblast-like synovial cells In vitro. Arthritis Rheum 31: 947-955, 1988.
11. Zinkernagel RM, Callahan GN, Althage A, Cooper S, Klein PA and Klein J: On the thymus in the differentiation of H-2 self-recognition by T cells: evidence for dual recognition? J Exp Med 147: 882-896, 1978.
12. Zinkernagel RM and Doherty PC: MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function and responsiveness Adv Immunol 27: 51-177, 1979.
13. Rock KL and Benacerraf B: MHC-restricted T cell activation: analysis with T cell hybridomas. Immunol Rev 76: 29-57, 1983.
14. Rock KL and Benacerraf B: Thymic T cells are driven to expand upon interaction with self-class II major histocompatibility complex gene products on accessory cells. Proc Natl Acad Sci USA 81: 1221-1224, 1984.
15. Penit C and Papiernik M: Regulation of thymocyte proliferation and survival by deoxynucleosides. Deoxycytidine produced by thymic accessory cells protects thymocytes from deoxyguanosine toxicity and stimulates their spontaneous proliferation. Eur J Immunol 16: 257-263, 1986.
16. Lanier LL: Lymphocyte surface antigens. Introduction. Fed Proc 44: 2863-2864, 1985.
17. 3+ T lymphocytes that express neither CD4 nor CD8 antigens. J Exp Med 164: 339-344, 1986.
18. Dardenne M and Bach JF: Thymic hormones. In: Kendall M, ed, The thymus gland. Academic Press, 1981; pp. 113-132.
19. Bach JF: Thymic hormones. Clin Immunol Allergy 3: 133-145, 1983.
20. Goldstein G: Thymic hormones. Triangle, 1972; 11: 7-14.
21. Goldstein G and Audhya TK: Thymopoietin to thymopentin: experimental studies. Surv Immunol Res 1985; 4 (Suppl.): 1-10.
22. Dardenne M, Pleau JM and Bach J-F: Evidence of the presence in normal serum of a carrier of the serum thymic factor (FTS). Eur J Immunol 10: 83-86, 1980.
23. Goldstein AL, Low TL, McAdoo M, McClure J, Thurman GB, Rossio J, Lai Ch-Y, Chang D, Wang S-S, Harvey C, Ramel AH Meienhofer J: Thymosin alphal: isolation and sequence analysis of an immunologically active thymic polypeptide. Proc Natl Acad Sci USA 74: 725-729, 1977.
24. Savino W and Dardenne M: Thymic hormone containing cells. VI. Immunohistologic evidence for the simultaneous presence of thymulin, thymopoietin and thymosin alpha-1 in normal and pathological human thymuses. Eur J Immunol 14: 987-991, 1984.
25. Wekerle H, Ketelsen U-P and Ernst M: Thymic nurse cells. Lymphoepithelial cell complexes in murine thymuses: morphological and serological characterization J Exp Med 151: 925-944, 1980.
26. Ritter MA, Sauvage CA and Cotmore SF: The human thymus microenvironment: in vivo identification of thymic nurse cells and other antigenically-distinct subpopulations of epithelial cells. Immunology 44: 439-446, 1981.
27. Kyewski BA and Kaplan HS: Lymphoepithelial interactions in the mouse thymus: phenotypic and kinetic studies on thymic nurse cells. J Immunol 128: 2287-2294, 1982.
28. Kyewski B, Hunsmann G, Friedrich R, Ketelsen UP and Wekerle H: Thymic nurse cells: intraepithelial thymocyte sojourn and its possible relevance for the pathogenesis of AKR lymphomas. Hamat Bluttransfus 26: 372-376, 1981.
29. Wijngaert FP van de, Rademakers LHPM, Schuurman H-J, Weger RA de and Kater L: Identification and in situ localization of the "thymic nurse cell" in man. J Immunol 130: 2348-2351, 1983.
30. Waal Malefijt Rde, Leene W, Roholl PJM, Wormmeester J and Hoeben KA: T cell differentiation within thymic nurse cells. Lab Invest, 1986; 55: 25-34.
31. Vakharia DD and Mitchison NA: Helper T cell activity demonstrated by thymic nurse T cells (TNC-T). Immunology 57: 269-273, 1984.
32. Fink PJ, Weissman IL, Kaplan HS and Kyewski BA: The immunocompetence of murine stromal cell-associated thymocytes. J Immunol 132: 2266-2272, 1984.
33. Fink PJ, Gallatin WM, Reichert RA, Butcher EC and Weissman IL: Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulation. Nature (London) 313: 233-235, 1985.
34. Boyd RL, Ward HA and Muller HK: Bursal and thymic reticular epithelial cells in the chicken: induction of B- and T-lymphocyte differentiation by in vitro monolayer cultures. J Reticuloendothel Soc 34: 383-393, 1983.
35. Andrews P, Boyd RL and Shortman K: The limited immuno-competence of thymocytes within murine thymic nurse cells. Eur J Immunol 15: 1043-1048, 1985.
36. Cantor H and Weissman I: Development and function of subpopulations of thymocytes and T lymphocytes. Prog Allergy 20: 1-64, 1976.
37. Mathieson BJ and Fowlkes BJ: Cell surface antigen expression on thymocytes: development and phenotypic differentiation of intrathymic subsets. Immunol Rev 82: 141-173, 1984.
38. Andrews P and Boyd RL: The thymic nurse cell: an isolated thymic microenvironment. Eur J Immunol 75: 36-42, 1985.
39. Emmart EW: A study of the histogenesis of the thymus in vitro. Anat Rec 66: 59-73, 1936.
40. + cortical thymocytes. Cell Immunol 97: 455-466, 1985.
41. Hofman FM, Modlin RL, Bhoopat L and Taylor CR: Distribution of cells bearing the Tac antigen during ontogeny of human lymphoid tissue. J Immunol 134: 3751-3755, 1985.
42. Maagd RA de, MacKenzie WA, Schuurman HJ, Ritter MA, Price KM, Broekhuizen R and Kater L: The human thymus microenvironment: heterogeneity detected by monoclonal anti-epithelial cell antibodies. Immunology 54: 745-754, 1985.
43. Nieburgs AC, Picciano PT, Korn JH, McCalister T, Allred C and Cohen S: In vitro growth and maintenance of two morphologically distinct populations of thymic epithelial cells. Cellul Immunol 90: 439-450, 1985.
44. Plum J and de Smedt M: Analysis of human prenatal and postnatal thymocytes with the anti-Tac monoclonal antibody and recombinant interleukin-2. Thymus 9: 149-158, 1987.
45. Papiernik M, Nabarra B and Bach JF: In vitro culture of functional thymic epithelium. Clin. Exp. Immunol 19: 281-287, 1975.
46. Waksal SD, Cohen IR, Waksal HW, Wekerle H, St Pierre RL and Feldman M: Induction of T-cell differentiation in vitro by thymus epithelial cells. Ann NY Acad Sci 249: 492-498, 1975.
47. Hsu SM, Raine L and Fanger H: Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29: 577-580, 1981.
48. Beard J: The origin and histogenesis of the thymus in Raja batis. Zoolog. Jahrbucher, Abt Anat Ontogenie Tiere 17: 403-480, 1902.
49. Bell ET: The development of the thymus. Arner J Anat 5: 29-62, 1906.
50. Hammar JA: Zur Histogenese und Involution der Thymusdruse. Anat. Anz 27: 23-30 und 41-80, 1905.
51. Hammar JA: Zur Frage der Histogenese der Thymusdruse. Zbl Pathol. 33-505-513, 1923.
52. Maximow A: Untersuchungen ueber Blut und Bindegewebe. IV. Ueber die Histogenese der Thymus bei Amphibien. Arch mikr Anat 79: 560-611, 1912.
53. Maximow A: Untersuchungen ueber Blut und Bindegewebe. V Ueber die embryonale Entwicklung der Thymus bei Selachiern. Arch mikr Anat 80: 39-88, 1912.
54. Bodey B: Histochemistry and histogenesis of the thymus in men during the prenatal development. PhD thesis, Sofia, 1977; pp. 1-360.
55. Bodey B and Hadjioloff AI: Thymus development, structure and function. Nature (Bulgaria), 1977; 26: 11-19.
56. Bodey B, Calvo W, Prummer O, Fliedner TM and Borysenko M: Development and histogenesis of the thymus in dog. A light and electronmicroscopical study 11: 227-238, 1987.
57. Le Douarin N: A biological cell labeling technique and its use in experimental embryology. Dev Biol. 50: 217-222, 1973.
58. Le Douarin N and Jotereau F: Embryologic origin of thymus lymphocytes in bird embryos. C R Acad Sci (Paris) 276: 629-632, 1973.
59. Le Douarin N and Jotereau FV: Tracing of cells of the avian thymus through embryonic life in interspecific chimeras. J Exp Med 142: 11-40, 1975.
60. Butcher EC: The regulation of lymphocyte traffic. Curr. Top. Microbiol Immunol 128: 85-122, 1986.
61. Kingston R, Jenkinson EJ and Owen JJ: A single stem cell can recolonize an embryonic thymus, producing phenotypically distinct T-cell populations. Nature (London) 317: 811-813, 1985.
62. Mulder AH and Visser JW: The entry of the prothymocyte into the thymus after lethal irradiation and bone marrow transplantation. I Seeding of bone marrow cells into the thymus. Thymus 11: 15-27, 1988.
63. Mulder AH, Visser JW, Zoetelief J and Bekkum DW van: The entry of the prothymocyte into the thymus after lethal irradiation and bone marrow transplantation. II. Time of entry. Thymus 11: 29-41, 1988.
64. Calvo W, Fliedner TM, Herbst EW, Hugl E and Bodey B: Degenerative changes and recovery of the thymus of lethally irradiated dogs, rescued by transfusion of cryopreserved autologous blood leukocytes. Exper Hemat 15: 1171-1178, 1987.
65. Fleker T: Tests with Temesvary's thymophysin. Polska gaz. lek 8: 537-539, 1929.
66. Nowinski VW: Beitrage zur Physiologie der Drusen; Fortgesetzte Beitrage zur Funktion der Thymus. Die Wirkungen des Thymocrescins auf das Wachstum. Biochem Ztschr 226: 415-428, 1930.
67. Bomskov Ch: Das Hormon des Thymus. Arch Klin Chir 200: 568-588, 1940.
68. Bomskov Ch and Sladovic L: Das Thymushormon und seine biologische Auswertung. Pflugers Arch 243: 611-622, 1941.
69. Monier JC, Dardenne M, Pleau JM, Schmitt D, Deschaux P and Bach J-F: Characterization of facteur thymique serique (FTS) in the thymus. I. Fixation of anti-FTS antibodies on thymic reticuloepithelial cells. Clin Exp Immunol 470-476, 1980.
70. 125I-labelled derivates of the "factor thymique serique". Hoppe Seylers Z Physiol Chem 363: 331-335, 1982.
71. Stahli C, Takacs B and Kocyba C: Monoclonal antibodies to thymosin alpha 1. Mol Immunol 20: 1095-1097, 1983.
72. Dates KK, Naylor PH and Goldstein AL: Localization of thymosin alpha 1 production to thymus medullary epithelial cells by use of monoclonal antibodies. Hybridoma 6: 47-59, 1987.
73. Fuccello A, Audhya T, Talle MA and Goldstcin G: Immunoassay for bovine serum thymopoietin: discrimination from splenin by monoclonal antibodies. Arch Biochem Biophys 225: 292-298, 1984.
74. Dardenne M, Pleau JM, Savino W and Bach J-F: Monoclonal antibody against the serum thymic factor (FTS). Immunol Lett 4: 79-83, 1982.
75. Mookerjee BK and Pauly JL: Human recombinant interleukin-2 is mitogenic to human lymphocytes. J Leukoc Biol 38: 553-556, 1985.
76. Nieburgs AC, Korn JH, Picciano PT and Cohen S: The production of regulatory cytokines for thymocyte proliferation by murine thymic epithelium in vitro. Cellul Immunol 90: 426-438, 1985.
77. Roosnek EE, Brouwer MC, Kipp JB and Aarden LA: Monocyte-dependent induction of proliferation of human peripheral T cells by recombinant interleukin 2. Eur J Immunol 16: 35-40, 1986.
78. Vakharia DD: Demonstration of keratin filaments in thymic nurse cells (TNC) and alloreactivity of TNC-T cell population. Thymus 5: 43-52, 1983.
79. Rocha Bl Lehuen A and Papiernik M: IL-2-dependent proliferation of thymic accessory cells. J Immunol 140: 1076-1080, 1988.
80. Munoz-Blay T, Nieburgs AC and Cohen S: Thymic epithelium in vitro. V. Binding of thymocytes to cultured thymic epithelial cells. Cellul Immunol 109: 371-383, 1987.
81. Munoz-Blay T, Rollo A, Cone RE and Cohen S: Characterization of the molecules involved in thymocyte thymic epithelial cell adhesion. Clin. Immunol Immunopathol 48: 343-353, 1988.
82. Picciano PT, Johnson B, Walenga RW, Donovan M, Borman BJ, Douglas WHJ and Kreutzer DL: Effects of D-valine on pulmonary artery endothelial cell morphology and function in cell culture. Exper Cell Res 151: 134-147, 1984.
83. Jerne NK: The somatic generation of immune recognition. Eur J Immunol, 1971; 9.
84. Gromkowski SH, Krensky AM, Martz E and Burakoff SJ: Functional distinctions between the LFA-1, LFA-2 and LFA-3 membrane proteins on human CTL are revealed with trypsin-pretreated target cells. J Immunol 134: 244-249, 1985.
85. Kyewski BA, Rouse RV and Kaplan HS: Thymocyte rosettes: multicellular complexes of lymphocytes and bone marrow-derived stromal cells in the mouse thymus. Proc Natl Acad Sci USA 79: 5646-5650, 1982.
86. Jason JM and Janeway CA: In vitro cultivation of nonlymphoid thymic cells: morphological and immunological characterization. Clin Immunol Immunopathol 30: 214-226, 1984.
87. Singer KH, Wolf LS, Lobach DF, Denning SM, Tuck DT, Robertson AL and Haynes BF: Human thymocytes bind to autologous and allogeneic thymic epithelial cells in vitro. Proc Natl Acad Sci USA 83: 6588-6592, 1986.
88. Longo DL and Schwartz RH: Gene complementation Neither Ir-Glphi gene need be present in the proliferative T cell to generate an immune response to Poly (Glu55Lys36Phe9)n. J Exp Med 151: 1452-1467, 1980.