메뉴 건너뛰기




Volumn 274, Issue 5292, 1996, Pages 1517-1520

Dependence of yeast pre-mRNA 3'-end processing on CFT1: A sequence homolog of the mammalian AAUAAA binding factor

Author keywords

[No Author keywords available]

Indexed keywords

CELL EXTRACT; CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR; MESSENGER RNA PRECURSOR; POLYCLONAL ANTIBODY; POLYNUCLEOTIDE ADENYLYLTRANSFERASE; PROTEIN SUBUNIT; SIGNAL PEPTIDE; TRANS ACTING FACTOR;

EID: 0029804177     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5292.1517     Document Type: Article
Times cited : (42)

References (37)
  • 1
    • 0026635941 scopus 로고
    • E. Wahle and W. Keller, Annu. Rev. Biochem. 61, 419 (1992); W. Keller, Cell 81, 829 (1995); E. Wahle, Biochim. Biophys. Acta 1261, 183 (1995).
    • (1992) Annu. Rev. Biochem. , vol.61 , pp. 419
    • Wahle, E.1    Keller, W.2
  • 2
    • 0029069868 scopus 로고
    • E. Wahle and W. Keller, Annu. Rev. Biochem. 61, 419 (1992); W. Keller, Cell 81, 829 (1995); E. Wahle, Biochim. Biophys. Acta 1261, 183 (1995).
    • (1995) Cell , vol.81 , pp. 829
    • Keller, W.1
  • 3
    • 0028988016 scopus 로고
    • E. Wahle and W. Keller, Annu. Rev. Biochem. 61, 419 (1992); W. Keller, Cell 81, 829 (1995); E. Wahle, Biochim. Biophys. Acta 1261, 183 (1995).
    • (1995) Biochim. Biophys. Acta , vol.1261 , pp. 183
    • Wahle, E.1
  • 7
    • 0025787944 scopus 로고
    • J. Lingner, J. Kellermann, W. Keller, Nature 354, 496 (1991); A. M. Zhelkovsky, M. Kessler, C. L. Moore, J. Biol. Chem. 270, 26715 (1995).
    • (1991) Nature , vol.354 , pp. 496
    • Lingner, J.1    Kellermann, J.2    Keller, W.3
  • 11
    • 0028589505 scopus 로고
    • L. Minvielle-Sebastia, B. Winsor, N. Bonneaud, F. Lacroute, Mol. Cell. Biol. 11, 3075 (1991); L. Minvielle-Sebastia, P. J. Preker, W. Keller, Science 266, 1702 (1994).
    • (1994) Science , vol.266 , pp. 1702
    • Minvielle-Sebastia, L.1    Preker, P.J.2    Keller, W.3
  • 12
    • 0024805402 scopus 로고
    • G. M. Gilmartin and J. R. Nevins. Genes Dev. 3, 2180 (1989); S. Bienroth, E. Wahle, C. Suter-Crazzolara, W. Keller, J. Biol. Chem. 266, 19768 (1991); K. G. K. Murthy and J. L. Manley. ibid. 267, 14804 (1992); A. Jenny and W. Keller, Nucleic Acids Res. 23, 2629 (1995).
    • (1989) Genes Dev. , vol.3 , pp. 2180
    • Gilmartin, G.M.1    Nevins, J.R.2
  • 13
    • 0026009674 scopus 로고
    • G. M. Gilmartin and J. R. Nevins. Genes Dev. 3, 2180 (1989); S. Bienroth, E. Wahle, C. Suter-Crazzolara, W. Keller, J. Biol. Chem. 266, 19768 (1991); K. G. K. Murthy and J. L. Manley. ibid. 267, 14804 (1992); A. Jenny and W. Keller, Nucleic Acids Res. 23, 2629 (1995).
    • (1991) J. Biol. Chem. , vol.266 , pp. 19768
    • Bienroth, S.1    Wahle, E.2    Suter-Crazzolara, C.3    Keller, W.4
  • 14
    • 0026694636 scopus 로고
    • G. M. Gilmartin and J. R. Nevins. Genes Dev. 3, 2180 (1989); S. Bienroth, E. Wahle, C. Suter-Crazzolara, W. Keller, J. Biol. Chem. 266, 19768 (1991); K. G. K. Murthy and J. L. Manley. ibid. 267, 14804 (1992); A. Jenny and W. Keller, Nucleic Acids Res. 23, 2629 (1995).
    • (1992) J. Biol. Chem. , vol.267 , pp. 14804
    • Murthy, K.G.K.1    Manley, J.L.2
  • 15
    • 0029131452 scopus 로고
    • G. M. Gilmartin and J. R. Nevins. Genes Dev. 3, 2180 (1989); S. Bienroth, E. Wahle, C. Suter-Crazzolara, W. Keller, J. Biol. Chem. 266, 19768 (1991); K. G. K. Murthy and J. L. Manley. ibid. 267, 14804 (1992); A. Jenny and W. Keller, Nucleic Acids Res. 23, 2629 (1995).
    • (1995) Nucleic Acids Res. , vol.23 , pp. 2629
    • Jenny, A.1    Keller, W.2
  • 18
    • 10544246716 scopus 로고    scopus 로고
    • note
    • 2-terminal hexahistidine tag and expressed in Escherichia coli strain BL21(DE3) (24). The recombinant protein was purified on nickel-nitrilotriacetic acid-agarose (Qiagen). Antisera were prepared in rabbits with the use of Ribi Adjuvant System (Sigma).
  • 19
    • 10544247613 scopus 로고    scopus 로고
    • The whole cell extract from S. cerevisiae was prepared as described (13)
    • The whole cell extract from S. cerevisiae was prepared as described (13).
  • 21
    • 0025334099 scopus 로고
    • J. S. Butler, P. P. Sadhale, T. Platt, Mol. Cell. Biol. 10, 2599 (1990); J. S. Bulter and T. Platt, Science 242, 1270 (1988)
    • (1988) Science , vol.242 , pp. 1270
    • Bulter, J.S.1    Platt, T.2
  • 22
    • 10544230470 scopus 로고    scopus 로고
    • note
    • Immunodepletion of the ammonium sulfate fractionated extract was performed as follows. Fifty microliters of preimmune sera or antisera to CFT1 protein or to PTF1 protein were coupled to 40 μl of protein A-Sepharose (Sigma), equilibrated with buffer D (6) containing 0.01% NP-40 for 2 hours at 4°C. After three washings, the ammonium sulfate-fractionated extract (50 μl) was added to the resin and incubated for 4 hours at 4°C on a rotating wheel.
  • 23
    • 10544229165 scopus 로고    scopus 로고
    • note
    • Standard in vitro processing reactions were performed in a volume of 10 μl as described (6, 13, 25). Reactions were incubated for 30 min at 30°C. Plasmids used for the in vitro transcription of the 3′-terminal region of CYC1 (pSKCYC) and ADH1 (pKSADHwt) were as described (26).
  • 25
    • 10544251279 scopus 로고    scopus 로고
    • note
    • Ammonium sulfate-fractionated extract and partially purified Mono Q fractions 14 to 24 were separated on an 8% SDS-polyacrylamide gel and analyzed by immunoblotting. Proteins were detected with antisera and reagents in the enhanced chemilumenescence kit (Amersham).
  • 27
    • 10544223069 scopus 로고    scopus 로고
    • note
    • Precleaved CYC1 RNA was obtained by gel purification of the upstream cleavage product of CYC1 pre-mRNA incubated for 45 min in an S. cerevisiae whole cell extract under standard processing conditions. To prevent polyadenylation of the upstream fragment, we replaced ATP with CTP, omitted magnesium, and included EDTA at a final concentration of 1 mM (6). Specific polyadenylation of precleaved RNA was assayed under the same conditions as for the standard cleavage and polyadenylation reaction (15) except that reactions were incubated for 60 min at 3O°C.
  • 36
    • 10544250887 scopus 로고    scopus 로고
    • note
    • For the reconstitution of specific polyadenylation, immunodepleted extract (2 μl) was mixed with 1 μl of partially purified PAP (contained in fraction 11) and 1 μl of Mono Q fractions 19 to 27. The PAP-containing fraction was identified by an unspecific polyadenylation assay as described (6).
  • 37
    • 10544239796 scopus 로고    scopus 로고
    • note
    • We thank J. Hani for advice with the computer search, U. Sterzer for technical assistance, H. Würl for materials, and A. Goppelt and K. Wiebauer for discussions and critical reading of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.