Removing monolayer cells from culture dishes by incubation with EDTA in studies of cell surface receptors

BioTechniques

Volumn 21, Issue 5, 1996, Pages 784-786

  Hu, Xiaotang ^a,b   Zuckerman, Kenneth S ^a  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

^b H LEE MOFFITT CANCER CENTER AND RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE RECEPTOR; COLONY STIMULATING FACTOR RECEPTOR; EDETIC ACID; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; PLASMID DNA; TRYPSIN;

ALPHA CHAIN; ANIMAL CELL; ARTICLE; CELL AGGREGATION; CELL COUNT; CELL LINE; CELL SUSPENSION; CELL VIABILITY; CULTURE MEDIUM; FLOW CYTOMETRY; GENETIC TRANSFECTION; MONOLAYER CULTURE; NONHUMAN; TECHNIQUE;

ANIMALIA;

EID: 0029799972     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96215bm06     Document Type: Article

References (4)

1. Gearing, D.P., J.A. King, N.M. Gough and N.A. Nicola. 1989. Expression cloning of a receptor for human granulocyte-macrophage colony-stimulation factor. EMBO J. 8:3367-3376.
2. Gluzman, Y. 1981. SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23:175-182.
3. Kreigler, M. 1990. Gene Transfer and Expression: A Laboratory Manual. Stockton Press, New York.
4. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.