Reproducibility of DNA ploidy and S-phase values from paraffin-embedded tissue

Analytical and Quantitative Cytology and Histology

Volumn 18, Issue 4, 1996, Pages 316-322

  Elavathil, Leelamma Jacob ^a,b   Celebre, Gino ^b   McFarlane, David ^c  

^a MCMASTER UNIVERSITY MEDICAL CENTRE   (Canada)

^b HAMILTON GENERAL HOSPITAL   (Canada)

^c Coulter Electronics of Canada Ltd ^*  (Canada)

Author keywords

DNA; flow cytometry; paraffin embedding; reproducibility of results

Indexed keywords

DNA; PARAFFIN;

ANEUPLOIDY; ARTICLE; CELL CYCLE S PHASE; COMPUTER PROGRAM; CONTROLLED STUDY; DIPLOIDY; EMBEDDING; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN TISSUE; OVARY CARCINOMA; PLOIDY; PRIORITY JOURNAL; REPRODUCIBILITY; TETRAPLOIDY; TISSUE FIXATION;

DNA, NEOPLASM; FEMALE; FLOW CYTOMETRY; HUMANS; OVARIAN NEOPLASMS; PARAFFIN EMBEDDING; PLOIDIES; REPRODUCIBILITY OF RESULTS; S PHASE;

FORMICIDAE; PHOENIX;

EID: 0029799943     PISSN: 08846812     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Alanen KA, Klemi PJ, Joensuu H, Kujari H, Pekkala E: Comparison of fresh, ethanol-preserved, and paraffin-embedded samples in DNA flow cytometry. Cytometry 1989;10:81-85
2. Alvcgard TA, Berg NO, Baldetorp B, Ferno M, Killander D, Ranstam J, Rydholm A, Akerman M: Cellular DNA content and prognosis of high grade soft tissue sarcoma: The Scandinavian sarcoma group experience. J Clin Oncol 1990;8: 538-547
3. Coon JS, Deitch AD, de Vere White RW, Koss LG, Melamed MR, Reeder JE, Weinstein RS, Wersto RP, Wheeless LL: Check samples for laboratory self-assessment in DNA flow cytometry. The National Cancer Institute's Flow Cytometry Network experience. Cancer 1989;63:1592-1599
4. Coon JS, Deitch AD, de Vere White RW, Koss LG, Melamed MR, Reeder JE, Weinstein RS, Wersto RP, Wheeless LL: Interinstitutional variability in DNA flow cytometric analysis of tumours: The National Cancer Institute's Flow Cytometry Network experience. Cancer 1988;61:126-130
5. Coon JS, Paxton H, Lucy L, Homburger H: Interlaboratory variation in DNA flow cytometry: Results of the College of American Pathologists Survey. Arch Pathol Lab Med 1994;118:681-685
6. Danesi DT, Spano M, Altavista P: Quality control study of the Italian group of cytometry on flow cytometry cellular DNA content measurement. Cytometry 1993;14:576-583
7. Engelholm SA, Spang-Thomsen M, Brunner N, Nohr I, Vindelov LL: Disaggregation of human solid rumours by combined mechanical and enzymatic methods. Br J Cancer 1985;51:93-98
8. Ferno M, Baldetorp B, Ewers SB, Idvall I, Olsson H, Sigurdsson H, Killander D: One or multiple samplings for flow cytometric DNA analysis in breast cancer: Prognostic implications. Cytometry 1992;13:241-249
9. Fossa SD, Berner A, Waehre H, Heiden T, Juul ME, van den Ouden D, Petterson EO, Wang N, Tribukait B: DNA ploidy in cell nuclei from paraffin-embedded material: Comparison of results from two laboratories. Cytometry 1992;13:395-403
10. Friedlander ML, Hedley DW, Swanson C, Russell P: Prediction of long-term survival by flow cytometric analysis of cellular DNA content in patients with advanced ovarian cancer. J Clin Oncol 1988;6:282-290
11. Frierson HF Jr: Flow cytometric analysis of ploidy in solid neoplasms: Comparison of fresh tissues with formalin-fixed paraffin-embedded specimens. Hum Pathol 1988;19:290-294
12. Frierson HF Jr: The need for improvement of flow cytometric analysis of ploidy and S-phase fraction. Am J Clin Pathol 1991;95:439-441
13. Giaretti W: Ploidy and proliferation evaluated by flow cytometry. An overview of techniques and impact in oncology. Tumori 1991;77:403-419
14. Hedley DW: Flow cytometry using paraffin-embedded tissue: Five years on cytometry. 1989;10:229-241
15. Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA: Method for analysis of cellular DNA content of paraffin embedded pathological material using flow cytometry. J Histochem Cytochem 1983;31:1333-1335
16. Hedley DW, Shankey VT, Wheeless LL: DNA cytometry consensus conference. Cytometry 1993;14:471
17. Heiden T, Strang P, Stendahl U, Tribukait B: The reproducibility of flow cytometric analysis in human tumours: Methodological aspects. Anticancer Res 1990;10:49-54
18. Herman CJ: Cytometric DNA analysis in the management of cancer: Clinical and laboratory considerations. Cancer 1992;69:1553-1556
19. Joensuu H, Kallioniemi OP: Different opinions on classification of DNA histograms produced from paraffin-embedded tissue. Cytometry 1989;10:711-717
20. Kallioniemi OP: Comparison of fresh and paraffinembedded tissue as starting material for DNA flow cytometry and evaluation of intra tumour heterogeneity. Cytometry 1988;9:164-169
21. Kallioniemi OP, Joensuu H, Klemi P, Koivula T: Inter-laboratory comparison of DNA flow cytometric results from paraffin-embedded breast carcinomas. Breast Cancer Res Treat 1990;17:59-61
22. Kallioniemi OP, Visakorpi T, Holli K, Heikkinen A, Isola J, Koivula T: Improved prognostic impact of S-phase values from paraffin-embedded breast and prostate carcinomas after correcting for nuclear slicing. Cytometry 1991;12: 413-421
23. Koss LG, Czerniak B, Herz F, Wersto RP: Flow cytometric measurements of DNA and other cell components in human tumours: A critical appraisal. Hum Pathol 1989,20:528-548
24. Kute TE, Gregory B, Galleshaw J, Hopkins M, Buss D, Case D: How reproducible are flow cytometry data from paraffin embedded blocks? Cytometry 1988;9:494-498
25. Listinsky CM, Bonfiglio TA, Leary J: Variable ploidy of ovarian clear cell carcinomas. Implications for adequacy of tissue sampling. Analyt Quant Cytol Histol 1988;10:21-27
26. Myc A, Tragonos F, Lara J, Melamed MR, Darzynkiewicz Z: DNA stainability in aneuploid breast tumours: Comparison of four DNA fluorochromes differing in binding properties. Cytometry 1992;13:389-394
27. Shankey TV, Rabinovitch PS, Bagwell B, Bauer KD, Duque RE, Hedley DW, Mayall BH, Wheeless L, Cox C: Guidelines for implementation of clinical DNA cytometry. Cytometry 1993;14:472-477
28. Silvestrini R: Quality control for evaluation of the S-phase fraction by flow cytometry: A multicentric study: The SICCAB group for quality control of cell kinetic determinations. Cytometry 1994;18:11-16
29. Van Thiel TP, Van der Linden JC, Baak JP, van de Sandt MM, van Galen C, Bezemer PD: Reproducibility of flow cytometric assessment of follicular tumours of the thyroid. J Clin Pathol 1989;42:260-263
30. Weaver DL, Bagwell CB, Hitchcox SA, Whetstone SD, Baker DR, Herbert DJ, Jones MA: Improved flow cytometric determination of proliferative activity (S phase fraction) from paraffin-embedded tissue. Am J Clin Pathol 1990;94: 576-584
31. Wersto RP, Liblit RL, Koss LG: Flow cytometric DNA analysis of human solid tumours. A review of interpretation of DNA histograms. Hum Pathol 1991;22:1085-1098
32. Wheeless LL, Coon JS, Cox C, Deitch AD, de Vere White RW, Koss LG, Melamed MR, O'Connell MJ, Reeder JE, Weinstein RS: Measurement variability in DNA flow cytometry of replicate samples. Cytometry 1989;10:731-738
33. Wheeless LL, Coon JS, Cox C, Deitch AD, de Vere White RW, Fradet Y, Koss LG, Melamed MR, O'Connell MJ, Reeder JE: Precision of DNA flow cytometry in inter-institutional analyses. Cytometry 1991;12:405-412
34. Williams NN, Daly JM: Flow cytometry and prognostic implications in patients with solid rumours. Surg Gynecol Obstet 1990;171:257-266