Flow cytometric analysis of nuclear matrix proteins: Method and potential applications

Cytometry

Volumn 24, Issue 4, 1996, Pages 348-359

  Dynlacht, Joseph R ^a,c   Henthorn, Jim ^a   O'Nan, Chad ^a   Dunn, S Terence ^a   Story, Michael D ^b  

^a UNIVERSITY OF OKLAHOMA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF TEXAS   (United States)

^c UNIVERSITY OF OKLAHOMA HEALTH SCIENCES CENTER   (United States)

Author keywords

Apoptosis; Camptothecin; Etoposide; Hyperthermia; Lamin B; Necrosis

Indexed keywords

CAMPTOTHECIN; ETOPOSIDE; LAMIN B; NUCLEAR PROTEIN;

APOPTOSIS; ARTICLE; CELL NUCLEUS MATRIX; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HYPERTHERMIA; LEUKEMIA CELL; MELANOMA CELL; NECROSIS; PRIORITY JOURNAL;

ANTIBODIES, MONOCLONAL; ANTIGENS, NUCLEAR; CELL CYCLE; DNA; DNA FRAGMENTATION; FLOW CYTOMETRY; FLUORESCENCE; HEAT; NUCLEAR PROTEINS; STAINING AND LABELING; TISSUE FIXATION; TUMOR CELLS, CULTURED;

EID: 0029795408     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19960801)24:4<348::AID-CYTO6>3.0.CO;2-B     Document Type: Article

References (54)

1. Afanasyev VN, Korol BA, Matylevich NP, Pechatnikov VA, Umansky SR: The use of flow cytometry for the investigation of cell death. Cytometry 14:603-609, 1993
2. Bidwell JP, Fey EG, van Wijnen AJ, Penman S, Stein JL, Lian JB, Stein GS: Nuclear matrix proteins distinguish normal diploid osteoblasts from osteosarcoma cells. Cancer Res 54:28-32, 1994.
3. Bladon T, Brasch K, Brown DL, Setterfield G: Changes in structure and protein composition of bovine lymphocyte nuclear matrix during concanavalin-A-induced mitogenesis. Biochem Cell Biol 66:40-53, 1988.
4. Burke B, Tooze J, Warren G: A monoclonal antibody which recognizes each of the nuclear lamin polypeptides in mammalian cells. EMBO J 2(3):361-367, 1983.
5. Casciola-Rosen LA, Miller DK, Anhalt GJ, Rosen A: Specific cleavage of the 70-kDa protein component of the ul small nuclear ribonucleoprotein is a characteristic biochemical feature of apoptotic cell death. J Biol Chem 269:30757-30760, 1994.
6. Chaly N, Bladon T, Setterfield G, Little JE, Kaplan JG, Brown DL: Changes in distribution of nuclear matrix antigens during the mitotic cell cycle. J Cell Biol 99:661-671, 1984.
7. Chu GL, Ross G, Wong RSL, Warters R, Dewey WC: Content of non-histone protein in nuclei after hyperthermic treatment. J Cell Physiol 154:217-221, 1993.
8. Clevenger CV, Bauer KD, Epstein JL: A method for simultaneous nuclear immunofluorescence and DNA content quantitation using monoclonal antibodies and flow cytometry. Cytometry 6:208-214, 1985.
9. Collins RJ, Harmon BV, Gobe GC, Kerr JFR: Internucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis. Int J Radiat Biol 61:451-453, 1992.
10. Compton DA, Cleveland DW: NuMA is required for the proper completion of mitosis. J Cell Biol 120:947-957, 1993.
11. Compton DA, Szilak I, Cleveland DW: Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis. J Cell Biol 116:1395-1408, 1992.
12. Crissman HA, Mullaney PF, Steinkamp SA: Methods and applications of flow systems for analysis and sorting of mammalian cells In: Methods in Cell Biology, Vol 9, Prescott DM (ed). Academic Press, New York, 1975, pp 179-246.
13. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F: Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808, 1992.
14. de Jong L, van Driel R, Stuurman N, Meijne AML, van Renswoude J: Principles of nuclear organization. Cell Biol Int Reports 14(12): 1051-1074, 1990.
15. Dewey WC, Ling CC, Meyn RE: Radiation-induced apoptosis: Relevance to radiotherapy. Int J Radiat Biol Oncol Phys 33:781-796, 1995.
16. Dworetzky SI, Fey EG, Penman S, Lian JB, Stein JL, Stein GS: Progressive changes in the protein composition of the nuclear matrix during rat osteoblast differentiation. Proc Natl Acad Sci USA 87:4605-4609, 1990.
17. Falcieri E, Gobbi P, Sabatelli P, Santi S, Farabegoli F, Rana R, Cataldi A, Maraldi NM, Martelli AM: A combined ultrastructural approach to the study of nuclear matrix thermal stabilization. Histochemistry 98:121-129, 1992.
18. Fernandes DJ, Catapano CV: Nuclear matrix targets for anticancer agents. Cancer Cells 3:134-140, 1991.
19. Fey EG, Penman S: Nuclear matrix proteins reflect cell type of origin in cultured human cells. Proc Natl Acad Sci USA 85:121-125, 1988.
20. Fey EG, Bangs P, Sparks C, Odgren P: The nuclear matrix: defining structural and functional roles. Crit Rev Eukaryot Gene Expr 1:127-143, 1991.
21. Hakes DJ, Berezney R: DNA binding properties of the nuclear matrix and individual nuclear matrix proteins. Evidence for salt-resistant DNA binding sites. J Biol Chem 266(17):11131-11140, 1991
22. Harmon BV, Corder AM, Collins RJ, Gobe GC, Allen J, Allan DJ, Kerr JFR: Cell death induced in a murine mastocytoma by 42-47°C heating in vitro: Evidence that the form of death changes from apoptosis to necrosis above a critical heat load. Int J Radiat Biol 58:845-858, 1990.
23. Harmon BV, Takano YS, Winterford CM, Gobe GC: The role of apoptosis in the response of tumours to mild hyperthermia. Int J Radiat Biol 59:489-501, 1991.
24. Henle KJ, Leeper DB: Effects of hyperthermia (45°C) on macromolecular synthesis in Chinese hamster ovary cells. Cancer Res 39: 2665-2770, 1979.
25. Kaufmann SH: Induction of endonucleolytic DNA cleavage in human acute myelogenous leukemia cells by etoposide, camptothecin, and other cytotoxic anticancer drugs: A cautionary note. Cancer Res 49:5870-5878, 1989.
26. Koopman G, Reutelingsperger CPM, Kuijten GAM, Keehnen RMJ, Pals ST, van Oers MUJ: Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84:1415-1420, 1994.
27. Krachmarov CP, Traub P: Heat-induced morphological and biochemical changes in the nuclear lamina from Ehrlich Ascites tumor cells in vivo. J Cell Biochem 52:308-319, 1993.
28. Krachmarov C, Tasheva B, Markov D, Hancock R, Dessev G: Isolation and characterization of nuclear lamina from Ehrlich Ascites tumor cells. J Cellular Biochem 30:351-359, 1986.
29. Landberg G, Roos G: Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis. Cancer Res 51:4570-4574, 1991.
30. Levitt D, King M: Methanol fixation permits flow cytometric analysis of immunofluorescent stained intracellular antigens. J Immunol Methods 96:233-237, 1987.
31. Li X, Traganos F, Darzynkiewicz Z: Simultaneous analysis of DNA replication and apoptosis during treatment of HL-60 cells with camptothecin and hyperthermia and mitogen stimulation of human lymphocytes. Cancer Res 54:4289-4293, 1994.
32. Lindquist S: The heat shock response. Annu Rev Biochem 55:1151-1191, 1986.
33. Lyderson BK, Pettijohn DE: Human-specific nuclear protein that associates with the polar region of the mitotic apparatus: Distribution in a human/hamster hybrid cell. Cell 22:489-499, 1980.
34. Mann GJ, Dyne M, Musgrove EA: Immunofluorescent quantification of ribonucleotide reductase M1 subunit and correlation with DNA content by flow cytometry. Cytometry 8:509-517, 1987.
35. Martelli AM, Falcieri E, Gobbi P, Manzoli L, Gilmour RS, Cocco L: Heat-induced stabilization of the nuclear matrix: A morphological and biochemical analysis in murine erythroleukemia cells. Exp Cell Res 196:216-225, 1991.
36. Miller TE, Beausang LA, Winchell LF, Lidgard GL: Detection of nuclear matrix proteins in serum from cancer patients. Cancer Res 52:422-427, 1992.
37. Miller TE, Beausang LA, Meneghini M, Lidgard GL: Death-induced changes to the nuclear matrix: The use of antinuclear matrix antibodies to study agents of apoptosis. Biotechniques 15:1042-1047, 1993.
38. Mullenders LHF, Venema J, van Hoffen A, Mayne LV, Natarajan AT, van Zeeland AA: The role of the nuclear matrix in DNA repair. In: Mutation and the Environment, Part A. New York, Wiley-Liss, 1990, pp 223-232.
39. Ornelles DA, Penman S: Prompt heat-shock and heat-shifted proteins associated with the nuclear matrix-intermediate filament scaffold in Drosophila melanogaster cells. J Cell Science 95:393-404, 1990.
40. Paddy MR, Belmont AS, Saumweber H, Agard DA, Sedat JW: Interphase nuclear envelope lamins form a discontinuous network that interacts with only a fraction of the chromatin in the nuclear periphery. Cell 62:89-106, 1990.
41. Partin AW, Getzenberg RH, CarMichael MJ, Vindivich D, Yoo J, Epstein JI, Coffey DS: Nuclear matrix protein patterns in human benign prostatic hyperplasia and prostate cancer. Cancer Res 53:744-746, 1993.
42. Reed LJ, Muench H: A simple method of estimating fifty per cent endpoints. Am J Hygiene 27:493-497, 1938.
43. Reiter T, Penman S: "Prompt" heat shock proteins: Translationally regulated synthesis of new proteins associated with nuclear matrix-intermediate filaments as an early response to heat shock. Proc Natl Acad Sci USA 80:4737-4741, 1983.
44. Sasaki K, Kurose A, Ishida Y: Flow cytometric analysis of the expression of PCNA during the cell cycle in HeLa cells and effects of the inhibition of DNA synthesis on it. Cytometry 14:876-882, 1993.
45. Schimenti KJ, Jacobberger JW: Fixation of mammalian cells for flow cytometric evaluation of DNA content and nuclear immunofluorescence. Cytometry 13:48-59, 1992.
46. Schindler M, Holland JF, Hogan M: Lateral diffusion in nuclear membranes. J Cell Biol 100:1408-1414, 1985.
47. Stewart C, Burke B: Teratocarcinoma stem cells and early mouse embryos contain only a single major lamin polypeptide closely resembling lamin B. Cell 51:383-392, 1987.
48. Tate EH, Wilder ME, Cram LS, Wharton W: A method for staining 3T3 cell nuclei with propidium iodide in hypotonic solution. Cytometry 4:211-215, 1983.
49. Ucker DS, Obermiller PS, Eckhart W, Apgar JR, Berger NA, Meyers J: Genome digestion is a dispensable consequence of physiological cell death mediated by cytotoxic T lymphocytes. Mol Cell Biol 12:3060-3069, 1992.
50. van Driel R, Humbel B, de Jong L: The nucleus: A black box being opened J Cell Biochem 47:311-316, 1991.
51. Verheijen R, Van Venrooij W, Ramaekers F: The nuclear matrix: Structure and composition. J Cell Science 90:11-36, 1988.
52. Vidair C, Dewey WC: Two distinct modes of hyperthermic cell death. Radiat Res 116:157-171, 1988.
53. Westra A, Dewey WC: Variation in sensitivity to heat shock during the cell-cycle of Chinese hamster cells in vitro. Int J Radiat Biol 19(5):467-477, 1971.
54. Wright WD, Higashikubo R, Roti Roti JL: Flow cytometric studies of the nuclear matrix. Cytometry 10:303-311, 1989.