Removal of RT-PCR inhibitors from RNA extracts of tissues

BioTechniques

Volumn 21, Issue 5, 1996, Pages 770-774

  Mathy, Nathalie L ^a,b   Lee, Rogan P ^a   Walker, John ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b Centre for Animal Biotechnology   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKINE; GAMMA INTERFERON; GUANIDINE DERIVATIVE; INTERLEUKIN 1ALPHA; LITHIUM CHLORIDE; MESSENGER RNA;

ANIMAL TISSUE; ARTICLE; CATTLE; CONTROLLED STUDY; EXTRACTION; GENE AMPLIFICATION; GENE EXPRESSION; LUNG PARENCHYMA; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SHEEP; TECHNIQUE;

ANIMALIA; BOS TAURUS; OVIS ARIES;

EID: 0029795316     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96215bm03     Document Type: Article

References (8)

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3. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
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8. Maliszewski, C.R., P.E. Baker, M.A. Schoenborn, B.S. Davis, D. Cosman, S. Gillis and D.P. Cerretti. 1988. Cloning, sequence and expression of bovine interleukin 1α and interleukin 1β complementary DNAs. Mol. Immunol. 25:429-437.