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note
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In complex mixtures containing lipids (e.g., tissue or cell homogenates), acetone (10:1 volume ratio) precipitation may be preferable to ultrafiltration. Acetone-precipitated proteins should be spun gently to prevent pellet compaction and resuspended in 50 mM potassium phosphate, pH 7.8, for overnight trypsinolysis as described in text.
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