Fidelity and predominant mutations produced by deep vent wild-type and exonuclease-deficient DNA polymerases during in vitro DNA amplification

DNA and Cell Biology

Volumn 15, Issue 7, 1996, Pages 589-594

  Huang, Hanxian ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE;

ARTICLE; BACTERIOPHAGE T4; GENE AMPLIFICATION; GENE DUPLICATION; GENE MUTATION; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

T4 BACTERIOPHAGE;

EID: 0029793878     PISSN: 10445498     EISSN: None     Source Type: Journal    
DOI: 10.1089/dna.1996.15.589     Document Type: Article

References (5)

1. ALBERTS, B.M., and FREY, L. (1970). T4 bacteriophage gene 32 a structural protein in the replication and recombination of DNA. Nature 227, 1313-1318.
2. CARIELLO, N.F., SWENBERG, T.A., and SKOPEK, T.R. (1991). Fidelity of the Thermococcus litoralis DNA polymerase (Vent) in polymerase chain reaction determined by denaturing gradient gel electrophoresis. Nucleic Acids Res. 19, 4193-4198.
3. CHASE, J.W., and WILLIAMS, K.R. (1986). Single-stranded DNA binding proteins required for DNA replication. Annu. Rev. Biochem. 55, 103-136.
4. ECKERT, K.A., and KUNKEL, T.A. (1991). The fidelity of DNA polymerases used in the PCR. In Polymerase Chain Reaction: A Practical Approach. M.J. McPherson, P. Quirke, and G.R. Taylor, eds. (IRL Press at Oxford University Press, Oxford) pp. 227-246.
5. FISCHER, S.G., and LERMAN, L.S. (1983). DNA fragments differing by single base-pair substitutions separated in denaturing gradient gel: correspondence with melting theory. Proc. Natl. Acad. Sci. USA 80, 1579-1583.