The effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system

Journal of Applied Bacteriology

Volumn 81, Issue 2, 1996, Pages 120-125

  Kinniment, S L ^a   Wimpenny, J W T ^a   Adams, D ^b   Marsh, P D ^b  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b Salisbury   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIMICROBIAL AGENTS; BACTERIA;

ACTINOMYCES; CHLORHEXIDINE; CLINICAL STUDY; DENTAL PLAQUES; LOW CONCENTRATIONS; MIXED CULTURES; MODELLING SYSTEMS; SHORT-PULSE; THREE ORDERS OF MAGNITUDE; VIABLE COUNT;

BIOFILMS;

CHLORHEXIDINE;

ACTINOMYCES; ARTICLE; BACTERICIDAL ACTIVITY; BACTERIUM ISOLATION; BIOFILM; CHEMOSTAT; FUSOBACTERIUM; MOUTH FLORA; PREDICTION; TOOTH PLAQUE;

ACTINOMYCES; ACTINOMYCES NAESLUNDII; BACTERIA (MICROORGANISMS); FUSOBACTERIUM; FUSOBACTERIUM NUCLEATUM; PORPHYROMONAS GINGIVALIS; VEILLONELLA DISPAR;

EID: 0029793291     PISSN: 00218847     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.1996.tb04488.x     Document Type: Article

References (32)

1. Adams, D. and Addy, M. (1994) Mouthrinses. Advances in Dental Research 8, 291-301.
2. Addy, M. (1986) Chlorhexidine compared with other locally delivered antimicrobials. A short review. Journal of Clinical Periodontology 13, 957-964.
3. Addy, M. (1990) Chemical plaque control. In Periodontics : A Practical Approach ed. Kieser, J.B. pp. 527-534. London: Wright.
4. Anwar, H. and Costerton, J.W. (1990) Enhanced activity of combination of tobramycin and piperacillin for eradication of sessile biofilm cells of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy 34, 1666-1671.
5. Anwar, H., Strap, J.L. and Costerton, J.W. (1992) Establishment of aging biofilms : possible mechanism of bacterial resistance to antimicrobial therapy. Antimicrobial Agents and Chemotherapy 36, 1347-1351.
6. Bonesvoll, P., Lökken, P. and Rölla, G. (1974) Influence of concentration, time, temperature and pH on the retention of chlorhexidine in the human oral cavity after mouth rinses. Archives of Oral Biology 19, 1025-1029.
7. Bradshaw, D.J., McKee, A.S. and Marsh, P.D. (1989a) Effect of carbohydrate pulses and pH on population shifts within oral microbial communities in vitro. Journal of Dental Research 68, 1298-1302.
8. Bradshaw, D.J., McKee, A.S. and Marsh, P.D. (1989b) The use of denned inocula stored in liquid nitrogen for mixed-culture chemostat studies. Journal of Microbiological Methods 9, 123-128.
9. Costerton, J.W., Cheng, K.-J., Geesey, G.G., Ladd, T.I., Nickel,
10. J.C., Dasgupta, M. and Marrie, TJ. (1987) Bacterial biofilms in nature and disease. Annual Review of Microbiology 41, 435-464.
11. Coulter, W.A. and Russell, C. (1976) Effect of chlorhexidine on plaque development in an artificial mouth, Microbios 16, 21 28.
12. Emilson, G.G. (1977) Susceptibility of various microorganisms to chlorhexidine. Scandinavian Journal of Dental Research 85, 255-265.
13. Emilson, C.G. (1994) Potential efficacy of chlorhexidine against mutans streptococci and human dental caries. Journal of Dental Research 73, 682-691.
14. Ganderton, L., Kinniment, S. and Wimpenny, J. (1995) Development and organisation of a steady state, nine-membered oral biofilm community. In The Life and Death of Biofilm éd. Wimpenny, J., Handley, P., Gilbert, P. and Lappin-Scott, H. pp. 37-43. Cardiff: BioLine.
15. Gristina, A.G., Hobgood, C.D., Webb, L.X. and Myrvik, Q,N. (1987) Adhesive colonisation of biomaterials and antibiotic resistance. Biomaterials 8, 423-426.
16. Grossman, E., Meckel, A.H., Isaacs, R.L., Ferretti, G.A., Sturzenberger, O.P., Bollmer, B.W., Moore, D.J., Lijana, R.C. and Manhart, M.D. (1989) A clinical comparison of antibacterial mouth rinses : effects of chlorhexidine, phenolics and sanguinarine on dental plaque and gingivitis. Journal of Perlodontology 60, 435-440.
17. Herles, S., Olsen, S. Afflitto, J. and Gaffar, A. (1994). Chemostat flow cell system : an in vitro model for the evaluation of antiplaque agents. Journal of Dental Research 73, 1748-1755.
18. Hull, P.S. (1980) Chemical inhibition of plaque. Journal of Clinical Periodontology 7, 431-442.
19. Kornman, K.S. (1986) The role of supragingival plaque in the prevention and treatment of periodontal diseases. A review of current concepts. Journal of Periodontal Research 21, (Suppl. 16), 5-22.
20. Marsh, P.D. (1992) Microbiological aspects of the chemical control of plaque and gingivitis. Journal of Dental Research 71, 1431-1438.
21. Marsh, P.D. and Bradshaw, DJ. (1993) Microbiological effects of new agents in dentifrices for plaque control. International Dental Journal 43, 399-406.
22. Mikkelsen, L., Jensen, S.B., Schiött, C.R. and Löe, H. (1981) Classification and prevalence of plaque streptococci after two years oral use of chlorhexidine. Journal of Periodontal Research 16, 646-658.
23. Millward, T.A. and Wilson, M. (1989) The effect of chlorhexidine on Streptococcus sanguis biofilms. Microbios 58, 155-164.
24. Moran, J., Addy, M. and Newcombe, R. (1988) The antibacterial effect of toothpastes on the salivary flora. Journal of Clinical Periodontology 15, 193-199.
25. Salem, A.M., Adams, D., Newman, H.N. and Rawle, L.W. (1987) Antimicrobial properties of 2 aliphatic amines and chlorhexidine in vitro and in saliva. Journal of Clinical Periodontology 14, 44-47.
26. Schaeken, M.J.M., de Jong, M.H., Franken, H.CM. and van der Hoeven, J.S. (1984) Effect of chlorhexidine and iodine on the composition of the human dental plaque flora. Caries Research 18, 401 407.
27. Schiött, C.R., Briner, W.W. and Löe, H. (1976) Two years use of oral chlorhexidine in man. II. The effect on the salivary bacterial flora. Journal of Periodontal Research 11, 145-152.
28. Stanley, A., Wilson, M. and Newman, H. N. (1989) The in vitro effects of chlorhexidine on subgingival plaque bacteria. Journal of Clinical Periodontology 16, 259-264.
29. ten Gate, J.M. and Marsh, P.D. (1994) Procedures for establishing efficacy of antimicrobial agents for chemotherapeutic caries prevention. Journal of Dental Research 73, 695-703.
30. Wilson, M. (1993) Laboratory assessment of antimicrobial agents for the treatment of chronic periodontitis. Microbial Ecology in Health and Disease 6, 143-145.
31. Wimpenny, J.W.T., Kinniment, S.L. and Scourfield, M.A. (1993) The physiology and biochemistry of biofilm. In Microbial Biofilms : Formation and Control, Society for Applied Bacteriology Technical Series, Vol. 30, ed. Denyer, S.P., Gorman, S.P. and Sussman, M. pp. 51-94, Oxford : Blackwell Science.
32. Zampatti, O., Roques, C. and Michel, G. (1994) An in vitro mouth model to test antiplaque agents : preliminary studies using a toothpaste containing chlorhexidine. Caries Research 28, 354-2.