Correlation of activity with stability of chemically modified ribozymes in nuclei suspension

Antisense and Nucleic Acid Drug Development

Volumn 6, Issue 2, 1996, Pages 111-118

  Heidenreich, Olaf ^a   Xu, Xiao ^a   Swiderski, Piotr ^b   Rossi, John J ^b   Nerenberg, Michael ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b LOMA LINDA UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEASE; NUCLEOSIDE; PHOSPHOROTHIOIC ACID; RIBONUCLEASE H; RIBOZYME; VIRUS RNA;

ANIMAL CELL; ARTICLE; CATALYSIS; CELL NUCLEUS; CONTROLLED STUDY; DRUG ACTIVITY; DRUG STABILITY; ENZYME ACTIVITY; FIBROBLAST; HUMAN T CELL LEUKEMIA VIRUS 1; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; VIRUS CELL TRANSFORMATION;

ANIMALIA; HTLV; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN T-LYMPHOTROPIC VIRUS 1; TRIXIS;

EID: 0029791662     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/oli.1.1996.6.111     Document Type: Article

References (6)

1. BEIGELMAN, L., McSWIGGEN, J.A., DRAPER, K.G., GONZALEZ, C., JENSEN, K., KARPEISKY, A.M., MODAK, A.S., MATULIC-ADAMIC, J., DIRENZO, A.B., HAEBERLI, P., SWEEDLER, D., TRACZ, D., GRIMM, S., WINCOTT, F.E., THACKRAY, V.G., and USMAN, N. (1995). Chemical modification of hammerhead ribozymes. J. Biol. Chem. 270, 25702-25708.
2. BRADFORD, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
3. GOTTEN, M., SCHAFFNER, G., and BIRNSTIEL, M.L. (1989). Ribozyme, antisense RNA and antisense DNA inhibition of U7 small nuclear ribonucleoprotein-mediated histone pre-mRNA processing in vitro. Mol. Cell. Biol. 9, 4479-4487.
4. DAGLE, J.M., WEEKS, D.L., and WALDER, J.A. (1991). Pathways of degradation and mechanism of action of antisense oligonucleotides in Xenopus laevis embryos. Antisense Res. Dev. 1, 11-20.
5. HEIDENREICH, O., BENSELER, F., FAHRENHOLZ, A., and ECKSTEIN, F. (1994). High activity and stability of hammerhead ribozymes containing 2′-modified pyrimidine nucleosides and phosphorothioates. J. Biol. Chem. 269, 2131-2138.
6. ZOUMADAKIS, M., NEUBERT, W.J., and TABLER, M. (1994). The influence of imperfectly paired helices I and III on the catalytic activity of hammerhead ribozymes. Nucleic Acids Res. 22, 5271-5278.