Quantitative PCR with colorimetric detection

Research in Immunology

Volumn 147, Issue 6, 1996, Pages 403-406

  Demay, F ^a   Darche, S ^a   Kourilsky, P ^a   Delassus, S ^a  

^a INSERM   (France)

Author keywords

IL10; quantitative PCR; actin

Indexed keywords

BETA ACTIN; INTERLEUKIN 10;

ARTICLE; COLORIMETRY; GENETIC TRANSCRIPTION; HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 0029790213     PISSN: 09232494     EISSN: None     Source Type: Journal    
DOI: 10.1016/0923-2494(96)82049-9     Document Type: Article

References (12)

1. Coutinho, G.C., Delassus, S., Kourilsky, P., Bandeira, A. & Coutinho, A. (1994), Developmental shift in the patterns of interleukin production in early post-natal life. Eur. J. Immunol., 24, 1858-1862.
2. Delassus, S., Coutinho, G.C., Saucier, C., Darche, S. & Kourilsky, P. (1994), Differential cytokine expression during murine gestation in placenta versus maternal blood. J. Immunol., 152, 2411-2420.
3. Fritz, E., Thiele, D., Willems, H. & Wittenbrink, M. (1995), Quantification of Coxiella burnetii by polymerase chain reaction (PCR) and a colorimetric microtiter plate hybridization assay (CMHA). Eur. J. Epidemiol., 11, 549-557.
4. Mulder, N., Mc Kinney, C., Christopherson, C., Sninsky, J., Greenfield, L. & Kwok, S. (1994), Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma. J. Clin. Microbiol., 32, 292-300.
5. Pannetier, C., Cochet, M., Darche, S. & Kourilsky, P. (1992), Une méthode de dosage quantitatif d'acides nucléiques par amplification enzymatique (méthode PCR) à saturation. C.R. Acad. Sci. Paris, 315, 271-277.
6. Pannetier, C., Delassus, S., Darche, S., Saucier, C. & Kourilsky, P. (1993), Quantitative titration of nucleic acids by enzymatic amplification reactions run to saturation. Nucleic Acids Res., 21, 577-583.
7. Ranki, M., Schätzl, H., Zachoval, R., Uusi-Oukari, M. & Lehtovaara, P. (1995), Quantification of Hepatitis virus DNA over a wide range from serum. Hepatology, 21, 1492-1499.
8. Shindo, M., Di Bisceglie, A., Silver, J., Limjoco, T., Hoofnagle, J. & Feinstone, S. (1994), Detection and quantitation of Hepatitis C virus RNA in serum using PCR and a colorimetric detection system. J. Virol. Methods, 48, 65-72.
9. Tartour, E., Gey, A., Sastre-Garau, X., Pannetier, C., Mosseri, V., Kourilsky, P. & Fridman, W. (1994), Analysis of interleukin 6 gene expression in cervical neoplasia using a quantitative polymerase chain reaction assay : evidence for enhanced interleukin 6 gene expression in invasive carcinoma. Cancer Res., 54, 6243-6248.
10. Tartour, E., Pannetier, C., Mathiot, C., Teillaud, J., Sautès, C., Kourilsky, P. & Fridman, W. (1995), Prognostic value of cytokine and soluble Fcg receptor assays in oncology. Immunol. Lett., 44, 145-148.
11. Yun, Z., Lundeberg, J., Johansson, B., Hedrum, A., Weiland, O., Uhlen, M. & Sönnerborg, A. (1994), Colorimetric detection of competitive PCR products for quantification of hepatitis C viremia. J. Virol. Methods, 41, 1-13.
12. Zou, W., Durand-Gasselin, I., Dulioust, A., Maillot, M., Galanaud, P. & Emilie, D. (1995), Quantification of cytokine gene expression by competitive PCR using a colorimetric assay. Eru. Cytokyne Netw., 6, 257-264.