Eucalypt α-tubulin: cDNA cloning and increased level of transcripts in ectomycorrhizal root system

Plant Molecular Biology

Volumn 31, Issue 4, 1996, Pages 905-910

  Diaz, Eugénie Carnero ^a   Martin, Francis ^a   Tagu, Denis ^a  

^a CEMAGREF   (France)

Author keywords

cytoskeleton; Eucalyptus globulus; gene expression; mycorrhiza; Pisolithus tinctorius; tubulin

Indexed keywords

BASIDIOMYCOTA; EUCALYPTUS; EUCALYPTUS GLOBULUS; PISOLITHUS TINCTORIUS;

EID: 0029789905     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00019477     Document Type: Article

References (22)

1. Burgess T, Laurent P, Dell B, Malajczuk N, Martin F: Effect of fungal-isolate aggressivity on the biosynthesis of symbiosis-related polypeptides in differentiating eucalypt ectomycorrhizas. Planta 195: 408-417 (1995).
2. Dexheimer J, Gérard J, Genet P: Study of transformations of the root system of Eucalyptus globulus associated with Pisolithus tinctorius. I. Aptitude to mycorrhization of different kinds of roots. Phytomorphology 44: 235-245 (1994).
3. Fosket DE, Morejohn LC: Structural and functional organization of tubulin. Annu Rev Plant Physiol 43: 201-240 (1992).
4. Guidet F, Rogowsky P, Taylor C, Weining S, Langridge P: Cloning and characterisation of a new rye-specific repeat sequence. Genome 34: 81-87 (1991).
5. Hilbert JL, Costa G, Martin F: Regulation of gene expression in ectomycorrhizas. Early ectomycorrhizins and polypeptide cleansing in eucalypt ectomycorrhizas. Plant Physiol 97: 977-984 (1991).
6. Kopczak SD, Haas NA, Hussey PJ, Silflow CD, Snustad DP: The small genome of Arabidopsis contains at least six expressed α-tubulin genes. Plant Cell 4: 539-547 (1992).
7. Logemann J, Schell J, Willmitzer L: Improved method for the isolation of RNA from plant tissues. Anal Biochem 163: 16-20 (1987).
8. Malajczuk N, Lapeyrie F, Garbaye J: Infectivity on pine and eucalypt isolates of Pisolithus tinctorius on roots of Eucalyptus urophylla in vitro. 1. Mycorrhiza formation in model systems. New Phytol 114: 627-631 (1990).
9. Martin F, Delaruelle C, Hilbert JL: An improved ergosterol assay to estimate fungal biomass in ectomycorrhizas. Mycol Res 94: 1059-1064 (1990).
10. Martin F, Laurent P, de Carvalho D, Burgess T, Murphy P, Nehls U, Tagu D: Fungal gene expression during ectomycorrhiza formation. Can J Bot 73 (Suppl. 1): S541-S547 (1995).
11. Montoliu Ll, Rigau J, Puigdomènech P: A tandem of α-tubulin genes preferentially expressed in radicular tissues from Zea mays. Plant Mol Biol 14: 1-15 (1989).
12. Nehls U, Martin F: Changes in root gene expression in ectomycorrhiza. In: Stocchi V, Bonfante P, Nuti M (eds) Biotechnology of Ectomycorrhizae: Molecular Approaches, pp. 125-137. Plenum Press, New York (1995).
13. Pelosi A, Lee MCS, Chandler SF, Hamill JD: Hormonal control of root primordia differentiation and root formation in cultured explants of Eucalyptus globulus seedlings. Aust J Plant Physiol 22: 409-415 (1995).
14. Rigau J, Capellades M, Montoliu Ll, Torres MA, Romera C, Martinez-Izquierdo JA, Tagu D, Puigdomènech P: Analysis of a maize α-tubulin gene promoter by transient expression and transgenic tobacco plants. Plant J 4: 1043-1050 (1993).
15. Rowland LJ, Nguyen B: Use of polyethylene glycol for purification of DNA from leaf tissue of woody plants. Biotechniques 14: 734-736 (1993).
16. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
17. Schatz PJ, Solomon F, Pillus L, Grisafi P, Botstein D: Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins. Mol Cell Biol 6: 3711-3721 (1986).
18. Short JM, Fernandez JM, Sorge JA, Hused WD: Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucl Acids Res 16: 7583-7600 (1988).
19. Stöcker M, Garcia-Mas J, Arus P, Messeguer R, Puigdomènech P: A highly conserved α-tubulin sequence from Prunus amygdalus. Plant Mol Biol 22: 913-916 (1993).
20. Tagu D, Python M, Crétin C, Martin F: Cloning symbiosis-related cDNAs from eucalypt ectomycorrhizas. New Phytol 125: 339-343 (1993).
21. Timonen S, Finlay RD, Söderström B, Raudaskoski M: Identification of cytoskeletal components in pine ectomycorrhizas. New Phytol 124: 83-92 (1993).
22. Timonen S, Söderström B, Raudaskoski M: Changes in the amounts of cytoskeletal proteins in pine roots and fungi during the development of ectomycorrhiza. Mycol Res, in press (1996).