Cautionary note on the use of dUMP-containing PCR primers with Pfu and Vent(R)® DNA polymerases

BioTechniques

Volumn 21, Issue 3, 1996, Pages 368-370

  Sakaguchi, Alan Y ^a   Sedlak, Michael ^a   Harris, J Mansel ^a   Sarosdy, Michael F ^a  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; URIDINE PHOSPHATE;

AGAR GEL ELECTROPHORESIS; ARCHAEBACTERIUM; ARTICLE; BASE MISPAIRING; GENE AMPLIFICATION; GENE MUTATION; NONHUMAN; POLYMERASE CHAIN REACTION;

ARCHAEA;

EID: 0029787964     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96213bm04     Document Type: Article

References (6)

1. Aslanidis, C. and P.J. deJong. 1990. Ligation independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18:6069-6074.
2. Eagle, L.R, X. Yin, A.R. Brothman, B.J. Williams, N.B. Atkin and E.V. Prochownik. 1995. Mutation of the MXI1 gene in prostate cancer. Nature Genet. 9:249-255.
3. Maniatis, T., E.F. Fritsch and J. Sambrook. 1982. Molecular Cloning, p. 454. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
4. Nisson, P.E., A. Rashtchian and P.C. Watkins. 1991. Rapid and efficient cloning of Alu-PCR products using uracil DNA glycosylase. PCR Methods Appl. 1:120-123.
5. Scott, B., K. Nielson, J. Cline and K. Kretz. 1994. High-fidelity PCR amplification with Pfu DNA polymerase. Strategies 7:62-63.
6. Zervos, A.S., J. Gyuris and R. Brent. 1993. Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites. Cell 72:223-232.