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9544221028
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2 = 10.36, P > 0.5). A significant but marginal deviation was detected in the P. crassifolia sample. A less biased estimator when allele frequencies are unequal gave the same estimated number of alleles (6).
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18
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9544237493
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note
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a equal to the estimated number of alleles in the population, determined from Eq. 2.
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19
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9544246172
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note
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2 (190 df) = 178, P > 0.5]. Accordingly, intraspecific phytogenies of allelic sequences were constructed, and the means and variances of the lengths of terminal branches were estimated. Again, one or more closely related sequences in P. crassifolia were omitted. Because P. crassifolia sequences are in general more closely related, this procedure resulted in a conservative test. Sequences examined in P. crassifolia were sequences PcS1-9, PcS11-13, PcS16, and PcS20-22.
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20
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9544240703
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note
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s = 0.90).
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9544226808
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note
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6, assuming that Nicotiana diverged from Physalis and Solanum 30 million years ago and a generation time of 2 years (see text).
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26
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9544222000
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note
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An S lineage was considered trans-generic if it inserted into the S-gene genealogy at a position ancestral to an allele found in Nicotiana [or Petunia, a more distantly related genus in the Solanaceae (21)]. The sensitivity of this estimate to uncertainty in the phylogeny was examined by use of the bootstrap. The data were resampled 100 times with replacement, and the number of transgeneric lineages was determined for each replicate.
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Supported by a grant from the Sloan Foundation (A.D.R.), NSF grants DEB-9404386 (A.D.R.), DEB-9306473 (J.R.K.), DEB-9527843 (J.R.K. and A.D.R.), a Hellman Fellowship (J.R.K.), and USPHS grant GM 37841 (M.K.U.). The assistance of the staff of the University of California Philip L. Boyd Deep Canyon Reserve is gratefully acknowledged.
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