Removal of template after random priming yields DNA probes of maximal specific activity

BioTechniques

Volumn 21, Issue 3, 1996, Pages 414-416

  Hammerl, Peter ^a   Thalhamer, Josef ^a  

^a Inst of Chemistry and Biochemistry   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DIAGNOSTIC ACCURACY; DNA PROBE; DNA TEMPLATE; NORTHERN BLOTTING; SOUTHERN BLOTTING;

EID: 0029787396     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96213bm16     Document Type: Article

References (7)

1. Brenig, B. and G. Brem. 1989. Integration and expression of a Somato-liberin-cDNA fusion gene construct in transgenic mice. Acta Endocrinol. Suppl. 120:137-138.
2. Brenig, B., M. Müller and G. Brem. 1989. A fast detection protocol for screening large numbers of transgenic animals. Nucleic Acids Res. 17:6422.
3. Feinberg, A.P. and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments a high specific activity. Anal. Biochem. 132:6-13.
4. Girvitz, S.C., S. Bacchetti, A.J. Rainbow and F.L. Graham. 1980. A rapid and efficient procedure for the purification of DNA from agarose gels. Anal. Biochem. 106:492-496.
5. Peloquin, J.J. and E.G. Platzer. 1991. A simple, inexpensive electroelution device for the recovery of nucleic acid fragments from agarose gels. BioTechniques 10:159-160.
6. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
7. Toth, L.E., K.L. Slawin, J.E. Pintar and M. Chi Ngujen-Huu. 1987. Region-specific expression of mouse homeobox genes in the mesodern and central nervous system. Proc. Natl. Acad. Sci. USA 84:6790-6794.