Catabolite repression resistance of gnt operon expression in Bacillus subtilis conferred by mutation of His-15, the site of phosphoenolpyruvate- dependent phosphorylation of the phosphocarrier protein HPr

Journal of Bacteriology

Volumn 178, Issue 18, 1996, Pages 5480-5486

  Reizer, Jonathan ^a   Bergstedt, Uta ^b   Galinier, Anne ^b   Küster, Elke ^c   Saier Jr , Milton H ^a   Hillen, Wolfgang ^c   Steinmetz, Michel ^d   Deutscher, Josef ^b,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^c CNRS   (France)

^d CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; PHOSPHOENOLPYRUVATE; PHOSPHOTRANSFERASE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; CATABOLITE REPRESSION; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE MUTATION; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 0029787307     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.18.5480-5486.1996     Document Type: Article

References (45)

1. Aymerich, S., G. Gonzy-Treboul, and M. Steinmetz. 1986. 5′-noncoding region sacR is the target of all identified regulation affecting the Icvansucrase gene in Bacillus subtilis. J. Bacteriol. 166:993-998.
2. Band, L., D. G. Yansura, and D. J. Henner. 1983. Construction of a vector for cloning promoters in Bacillus subtilis. Gene 930:313-315.
3. Chambliss, G. H. 1993. Carbon source-mediated catabolite repression, p. 213-219. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus sublilis and other gram-positive bacteria: biochemistry, physiology, and molecular genetics. American Society for Microbiology. Washington, D.C.
4. Crutz, A. M., M. Steinmetz, S. Aymerich, R. Richter, and D. Le Coq. 1990. Induction of Icvansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system. J. Bacteriol. 172:1043-1050.
5. Glc of the phosphoenolpyruvatexarbohydrate phosphotransferase system. J. Bacteriol. 167:393-395.
6. Deutscher, J., and R. Engelmann. 1984. Purification of an ATP-dependent protein kinase from Streptococcus faecalis. FEMS Microbiol. Lett. 23:157-162.
7. Deutscher, J., U. Kessler, C.-A. Alpert, and W. Hengstenberg. 1984. Bacterial phosphoenolpyruvate-dependent phosphotransferase system: P-Ser-HPr and its possible regulatory function. Biochemistry 23:4455-4460.
8. Deutscher, J., U. Kessler, and W. Hengstenberg. 1985. Streptococcal phosphoenolpyruvate:sugar phosphotransferase system: purification and characterization of a phosphoprotein phosphatase which hydrolyzes the phosphoryl bond in seryl-phosphorylated histidine-containing protein. J. Bacteriol. 163:1203-1219.
9. Deutscher, J., E. Küster, U. Bergstedt, V. Charrier, and W. Hillen. 1995. Protein kinase-dependent HPr/CcpA interaction links glycolytic activity to catabolite repression in Gram-positive bacteria. Mol. Microbiol. 15:1049-1053.
10. Deutscher, J., B. Pevec, K. Beyreuther, H.-H. Kiltz, and W. Hengstenberg. 1986. Streptococcal phosphoenolpyruvate-sugar phosphotransferase system: amino acid sequence and site of ATP-dependent phosphorylation of HPr. Biochemistry 25:6543-6551.
11. Deutscher, J., J. Reizer, C. Fischer, A. Galinier, M. H. Saier, Jr., and M. Steinmetz. 1994. Loss of protein kinase-calalyzed phosphorylation of HPr, a phosphocarrier protein of the phosphotransferase system, by mutation of the ptsH gene confers catabolite repression resistance to several catabolic genes of Bacillus subtilis. J. Bacteriol. 176:3336-3344.
12. Deutscher, J., and M. H. Saier, Jr. 1983. ATP-dependent. protein kinase-catalyzed phosphorylation of a seryl residue in HPr, the phosphoryl carrier protein of the phosphotransferase system in Streptococcus pyogenes. Proc. Natl. Acad. Sci. USA 80:6790-6794.
13. Deutscher, J., and H. Sauerwald. 1986. Stimulation of dihydroxyacetone and glycerol kinase activity in Streptococcus faecalis by phosphoenolpyruvate-dependent phosphorylation catalyzed by enzyme 1 and HPr of the phosphotransferase system. J. Bacteriol. 166:829-836.
14. Eisermann, R., J. Deutscher, G. Gonzy-Treboul, and W. Hengstenberg. 1988. Site-directed mutagenesis with the ptsH gene of Bacillus subtilis: isolation and characterization of heat-stable proteins altered at the ATP-dependent regulatory phosphorylation site. J. Biol. Chem. 263:17050-17054.
15. Fujita, V., and Y. Miwa. 1994. Catabolite repression of the Bacillus subtilis gnt operon mediated by the CcpA protein. J. Bacteriol. 176:511-513.
16. Fujita, Y., Y. Miwa, A. Galinier, and J. Deutscher. 1995. Specific recognition of the Bacillus subtilis gnt cis-acting catabolite responsive element by a protein complex formed between CcpA and seryl-phosphorytated HPr. Mol. Microbiol. 17:953-960.
17. 31P nuclear-magnetic-resonance studies on the phosphocarrier protein HPr, phosphohistidines and phosphorylated HPr. Eur. J. Biochem. 75:287-296.
18. Hengstenberg, W., W. K. Penberthy, K. L. Hill, and M. L. Morse. 1969. Phosphotransferase system of Staphylococcus aureus: its requirement for the accumulation and metabolism of galactosides. J. Bacteriol. 99:383-388.
19. Henkin, T. M., F. J. Grundy, W. L. Nicholson, and G. H. Chambliss. 1991. Catabolite repression of α-amylase gene expression in Bacillus subtilis involves a transacting gene product homologous to the Escherichia coli lact and galR repressors. Mol. Microbiol. 5:575-584.
20. Hueck, C. J., W. Hillen, and M. H. Saier, Jr. 1994. Analysis of a cis-active sequence mediating catabolite repression in Gram-positive bacteria. Res. Microbiol. 145:503-518.
21. Jones, B., R. Klevit, W. Hillen, A. Galinier, and J. Deutscher. 1996. Unpublished data.
22. Nicholson, W. L., Y.-K. Park, T. M. Henkin, M. Won, M. J. Weickert, J. A. Gaskell, and G. H. Chambliss. 1987. Catabolite repression-resistant mutations of the Bacillus subtilis alpha-amylase promoter affect transcription levels and are in an operator-like sequence. J. Mol. Biol. 198:609-618.
23. Nihashi, J.-I., and Y. Fujita. 1984. Catabolite repression of inositol dehydrogenase and gluconate kinase synthesis in Bacillus subtilis. Biochim. Biophys. Acta 798:88-95.
24. Postma, P. W., J. W. Lengeler, and G. R. Jacobson. 1993. Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol. Rev. 57:543-594.
25. Reizer, J., J. Deutscher, F. Grenier, J. Thompson, W. Hengstenberg, and M. H. Saier, Jr. 1988. The phosphoenolpyruvate:sugar phosphotransferase system in Gram-positive bacteria: properties, mechanism and regulation. CRC Crit. Rev. Microbiol. 15:297-338.
26. Reizer, J., M. J. Novotny, W. Hengstenberg, and M. H. Saier, Jr. 1984. Properties of ATP-dependent protein kinase from Streptococcus pyogenes that phosphorylates a scryl residue in HPr, a phosphocarrier protein of the phosphotransferase system. J. Bacteriol. 160:333-340.
27. Reizer, J., M. J. Novotny, I. Stuiver, and M. H. Saier, Jr. 1984. Regulation of glycerol uptake by the phosphoenolpyruvate-sugar phosphotransferase system in Bacillus subtilis. J. Bacteriol. 159:243-250.
28. Reizer, J., S. L. Sutrina, M. H. Saier, Jr., G. C. Stewart, A. Peterkofsky, and P. Reddy. 1989. Mechanistic and physiological consequences of HPr(ser) phosphorylation on the activities of the phosphoenolpyruvate:sugar phosphotransferase system in Gram-positive bacteria: studies with site-specific mutants. EMBO J. 8:2111-2120.
29. Reizer, J., S. L. Sutrina, L.-F. Wu, J. Deutscher, P. Reddy, and M. H. Saier, Jr. 1992. Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli. J. Biol. Chem. 267:9158-9169.
30. Saier, M. H., Jr. 1989. Protein phosphorylation and allosteric control of inducer exclusion and catabolite repression by the bacterial phosphoenolpyruvate:sugar phosphotransferase system. Microbiol. Rev. 53:109-120.
31. Saier, M. H., Jr. 1993. Regulatory interactions involving the proteins of the phosphotransferase system in enteric bacteria. J. Cell. Biochem. 51:62-68.
32. Steinmetz, M., D. le Coq, and S. Aymerich. 1989. Induction of saccharolytic enzymes by sucrose in Bacillus subtilis: evidence for two partially interchangeable regulatory pathways. J. Bacteriol. 171:1519-1523.
33. Stülke, J., I. Martin-Verstraete, V. Charrier, A. Klier, J. Deutscher, and G. Rapoprt. 1995. The HPr protein of the phosphotransferase system links induction and catabolite repression of the Bacillus subtilis levanase operon. J. Bacteriol. 177:6928-6936.
34. Vadeboncoeur, C., D. Brochu, and J. Reizer. 1991. Quantitative determination of the intracellular concentration of the various forms of HPr, a phosphocarrier protein of the phosphoenolpyruvate:sugar phosphotransferase system in growing cells of oral streptococci. Anal. Biochem. 196:24-30.
35. Weickert, M. J., and S. Adhya. 1992. A family of bacterial regulators homologous to Gal and Lac repressors. J. Biol. Chem. 267:15869-15874.
36. Weinrauch, Y., T. Msadek, F. Kunst, and D. Dubnau. 1991. Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis. J. Bacteriol. 173:5685-5690.
37. Wittekind, M., J. Reizer, J. Deutscher, M. H. Saier, Jr., and R. E. Klevit. 1989. Common structural changes accompany the functional inactivation of HPr by seryl phosphorylation or by serine to aspartate substitution. Biochemistry 28:9908-9912.
38. + symporter by metabolite-activated ATP-dependent phosphorylation of HPr in Lactobacillus brevis. J. Bacteriol. 176:3484-3492.
39. + symport in Lactobacillus brevis. Proc. Natl. Acad. Sci. USA 91:3102-3106.
40. Ye, J. J., J. Reizer, X. Cui, and M. H. Saier, Jr. 1994. Inhibition of the phosphoenolpyruvate:lactose phosphotransferase system and activation of a cytoplasmic sugar-phosphate phosphatase in Lactococcus lactis by ATP-dependent metabolite-activated phosphorylation of serine-46 in the phosphocarrier protein HPr. J. Biol. Chem. 269:11837-11844.
41. Ye, J. J., J. Reizer, and M. H. Saier, Jr. 1994. Regulation of 2-deoxyglucose accumulation in Lactococcus lactis vesicles by metabolite-activated. ATPdependent phosphorylation of serine-46 in HPr of the phosphotransferase system. Microbiology 140:3421-3429.
42. + permease of Lactobacillus brevis. Proc. Natl. Acad. Sci. USA 92:417-421.
43. Ye, J. J., and M. H. Saier, Jr. 1995. Allosteric regulation of the glucose:H symporter of Lactobacillus brevis: cooperative binding of glucose and HPr(Ser-P). J. Bacteriol. 177:1900-1902.
44. Ye, J. J., and M. H. Saier, Jr. 1995. Purification and characterization of a small membrane-associated sugar phosphate phosphatase that is allosterically activated by HPr(Ser-P) of the phosphotransferase system in Lactococcus lactis. J. Biol. Chem. 270:16740-16744.
45. Ye, J. J., and M. H. Saier, Jr. 1995. Unpublished data.