Isolation, cloning and gel blot analysis of high molecular weight wheat DNA

BioTechniques

Volumn 21, Issue 4, 1996, Pages 572-576

  Gill, Kulvinder S ^a   Nasuda, Shuhai ^a   Gill, Bikram S ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PLANT DNA;

ARTICLE; DNA DETERMINATION; MOLECULAR CLONING; NONHUMAN; PLANT LEAF; WHEAT;

TRITICUM AESTIVUM;

EID: 0029785316     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96214bm01     Document Type: Article

References (7)

1. Ganal, M.W. and S.D. Tanksley. 1989. Analysis of tomato DNA by pulsed-field gel electrophoresis. Plant Mol. Biol. Rep. 7:17-28.
2. Gill, K.S., B.S. Gill, T.R. Endo and Y. Mukai. 1993. Fine physical mapping of Ph1, a chromosome pairing regulator gene in polyploid wheat. Genetics 134:1231-1236.
3. Gill, K.S., E.L. Lubbers, B.S. Gill, W.J. Raupp and T.S. Cox. 1991. A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34:362-374.
4. Guidet, F., P. Rogowsky and P. Langridge. 1990. A rapid method of preparing megabase plant DNA. Nucleic Acids Res. 18:4955.
5. Röder, M.S., M.E. Sorrells and S.D. Tanksley. 1992. 5S ribosomal gene clusters in wheat: pulsed field gel electrophoresis reveals a high degree of polymorphism. Mol. Gen. Genet. 232:215-220.
6. Tanksley, S.D., M.W. Ganal and G.B. Martin. 1995. Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes. Trends Genet. 11:63-68.
7. Woo, S.-S., J. Jiang, B.S. Gill, A.H. Paterson and R.A. Wing. 1994. Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor. Nucleic Acids Res. 22:4922-4937.