RT-PCR analysis of MMP-9 expression in human articular cartilage chondrocytes and synovial fluid cells

Biotechnic and Histochemistry

Volumn 71, Issue 4, 1996, Pages 208-213

  Tsuchiya, K ^a   Maloney, W J ^a   Vu, T ^b   Hoffman, A R ^b   Schurman, D J ^a   Smith, R L ^a,b,c  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VA PALO ALTO HEALTH CARE SYSTEM   (United States)

^c STANFORD UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

articular cartilage; chondrocytes; matrix metalloproteinase 9; RT PCR; synovial fluid cells

Indexed keywords

GELATINASE B; MESSENGER RNA;

ADULT; AGED; ARTICLE; ARTICULAR CARTILAGE; CARTILAGE CELL; CARTILAGE DEGENERATION; CELL METABOLISM; CONTROLLED STUDY; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; MALE; NORTHERN BLOTTING; OSTEOARTHRITIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SYNOVIAL FLUID;

EID: 0029784163     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.3109/10520299609117161     Document Type: Article

References (29)

1. Aschoff, J. M., Lazarus, D., Fanburg, B. L. and Lanzillo, J. J. 1994. Relative quantification of angiotensin-converting enzyme mRNA in human smooth muscle cells, monocytes, and lymphocytes by the polymerase chain reaction. Anal. Biochem. 219: 218-223.
2. Campbell, I. K., Golds, E. E., Mort, J. S. and Roughley, P. J. 1986a. Human articular cartilage secretes characteristic metal dependent proteinases upon stimulation by mononuclear cell factor. J. Rheumatol. 13: 20-27.
3. Campbell, I. K., Roughley, P. J. and Mort, J. S. 1986b. The action of human articular-cartilage metalloproteinase on proteoglycan and link protein. Biochem. J. 237: 117-122.
4. Chelly, J., Kaplan, J. C., Maire, P., Gautron, S. and Kahn, A. 1988. Transcription of the dystrophin gene in human muscle and non-muscle tissues. Nature 333: 858-860.
5. Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol chloroform extraction. Anal. Biochem. 162: 156-159.
6. Dean, D. D. 1991. Proteinase-mediated cartilage deg-radation in osteoarthritis. Semin. Arthritis Rheum. 20 (suppl 2), 2-11.
7. Dean, D. D., Martel-Pelletier, J., Pelletier, J. P., Howell, D. S. and Woessner, J. F., Jr. 1989. Evidence for metalloproteinase and metalloproteinase inhibitor imbalance in human osteoarthritic cartilage. J. Clin. Invest. 84: 678-685.
8. Gilliland, G., Perrin, S., Blanchard, K. and Bunn, H. F. 1990. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc. Natl. Acad. Sci. USA 87: 2725-2729.
9. Hamerman, D. 1989. The biology of osteoarthritis. N. Engl. J. Med. 320: 1322-1330.
10. Karet, F. E., Charnock-Jones, D. S., Harrison-Woolrych, M. L., O'Reilly, G., Davenport, A. P. and Smith. S. K. 1994. Quantification of mRNA in human tissue using fluorescent nested reversetranscriptase polymerase chain reaction. Anal. Biochem. 220: 384-390.
11. Kinoshita, T., Imamura, J., Nagai, H. and Shimotohno, K. 1992. Quantification of gene expression over a wide range by the polymerase chain reaction. Anal. Biochem. 206: 231-235.
12. Martel-Pelletier, J., Pelletier, J. P., Cloutier, J. M., Howell, D. S., Ghandur-Mnaymneh, L. and Woessner, J. F., Jr. 1984. Neutral proteases capable of proteoglycan digesting activity in osteoarthritic and normal human articular cartilage. Arthritis Rheum. 27: 305-312.
13. McCachren, S. S. 1991. Expression of metalloproteinases and metalloproteinase inhibitor in human arthritic synovium. Arthritis Rheum. 34: 1085-1093.
14. Mohtai, M., Smith, R. L., Schurman, D. J., Tsuji, Y., Torti, F. M. Hutchinson, N. I. Stetler-Stevenson, W. G. and Goldberg, G. I. 1993. Expression of 92-kD collagenase/gelatinase (gelatinase B) in osteoarthritic cartilage and its induction in normal human articular cartilage by interleukin. 1. J. Clin. Invest. 92: 179-185.
15. Nguyen, Q., Murphy, G., Roughley, P. J. and Mort, J. S. 1989. Degradation of proteoglycan aggregate by a cartilage metalloproteinase. Biochem. J. 259: 61-67.
16. Noonan, K. E. and Roninson, I. B. 1988. mRNA phenotyping by enzymatic amplification of random primed CDNA. Nucleic Acids Res. 16: 10366.
17. Noonan, K. E., Beck, C., Holzmayer, T. A., Chin, J. E., Wunder, J. S., Andrulis, I. L., Gazdar, A. F., Willman, C. L., Griffith, B., Von Hoff, D. D. and Roninson, I. B. 1990. Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction. Proc. Natl. Acad. Sci. USA 87: 7160-7164.
18. Okada, Y., Nagase, H. and Harris, E. D. Jr. 1986. A metalloproteinase from human rheumatoid synovial fibroblasts that digests connective tissue matrix components. J. Biol. Chem. 261: 14245-14255.
19. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B. and Erlich, H. A. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-491.
20. Sanger, F., Nicklen. S. and Coulson. A. R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467.
21. Senior. R. M., Griffin, G. L., Fliszar, C. J., Shapiro, S. D., Goldberg, G. I. and Welgus, H. G. 1991. Human 92- and 72-kilodalton type IV collagenases are elastases. J. Biol. Chem. 266: 7870-7875.
22. Sugimoto, T. Fujita, M., Taguchi, T. and Morita, T. 1993. Quantitative detection of DNA by coamplification polymerase chain reaction: a wide detectable range controlled by the thermodynamic stability of primer template duplexes. Anal. Biochem. 211: 170-172.
23. Vu, T. H. and Hoffman, A. R. 1994. Promoter-specific imprinting of human insulin-like growth factor II gene. Nature 371: 714-717.
24. Vu, T. H., Yballe, C., Boonyanit, S. and Hoffman. A. R. 1995. Insulin-like growth factor II in uterine smooth-muscle tumors: maintenance of genomic imprinting in leiomyomata and loss of imprinting in leiomyosarcomata. J. Clin. Endocrinol. Metab. 80: 1670-1676.
25. Wang, A. M., Doyle, M. V. and Mark, D. F. 1989. Quantitation of mRNA by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA 86: 9717-9721.
26. Werb, Z. 1989. Proteinases and matrix degradation. In: Textbook of Rheumatology: Proteinases and Matrix Degradation. Kelly, W. N., Harris, E. D., Jr., Ruddy, S. and Sledge, C. B., Eds. W. B. Saunders Co., Philadelphia, pp. 300-321.
27. Wilhelm, S. M., Collier, I. E., Marmer, B. L., Eizen, A. Z., Grant, G. A. and Goldberg, G. I. 1989. SV40-transformed human lung fibroblasts secrete 92-kDa type IV collagenase which is identical to that secreted by normal human macrophages. J. Biol. Chem. 264: 17213-17221.
28. Woessner, J. F., Jr. and Selzer, M. G. 1984. Two latent metalloproteases of human articular cartilage that digest proteoglycan. J. Biol. Chem. 259: 3633-3638.
29. Zafarullah, M., Pelletier, J. P., Cloutier, J. M. and Martel-Pelletier, J. 1993. Elevated metalloproteinase and tissue inhibitor of metalloproteinase mRNA in human osteoarthritic synovia. J. Rheu.