Prolonged final extension time increases cloning efficiency of PCR products

BioTechniques

Volumn 21, Issue 2, 1996, Pages 192-196

  Li, Qin Bao ^a   Guy, Charles L ^a,b  

^a UNIVERSITY OF FLORIDA   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RESTRICTION ENDONUCLEASE;

AMINO ACID SEQUENCE; ARTICLE; DNA DETERMINATION; ENZYME ACTIVITY; GENE INSERTION; MOLECULAR CLONING; POLYMERASE CHAIN REACTION;

EID: 0029784030     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96212bm04     Document Type: Article

References (5)

1. Clark, J.M. 1988. Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res. 16:9677-9686.
2. Costa, G.L. and M.P. Weiner. 1994. Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments. Nucleic Acids Res. 22:2423.
3. Hu, G. 1993. DNA polymerase-catalyzed addition of nontemplated extra nucleotides to the 3′ end of a DNA fragment. DNA Cell Biol. 12:763-770.
4. Li, Q.B., Q. Cai and C.L. Guy. 1994. A DNA extraction method for RAPD analysis from plant rich in soluble polysaccharides. Plant Mol. Biol. Reporter 11:122-127.
5. Robles, J. and M. Doers. 1994 T-vector tips. Promega Notes 45:19-20.