Nucleotide level detection of cyclobutane pyrimidine dimers using oligonucleotides and magnetic beads to facilitate labelling of DNA fragments incised at the dimers and chemical sequencing reference ladders

Carcinogenesis

Volumn 17, Issue 8, 1996, Pages 1549-1552

  Li, Shisheng ^a   Waters, Raymond ^a  

^a SWANSEA UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BIOTIN; CYCLOBUTANE DERIVATIVE; DNA FRAGMENT; ENDONUCLEASE; OLIGONUCLEOTIDE; PYRIMIDINE DIMER; RESTRICTION ENDONUCLEASE; STREPTAVIDIN;

ARTICLE; ESCHERICHIA COLI; ISOTOPE LABELING; MAGNETISM; MICROCOCCUS LUTEUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; ULTRAVIOLET IRRADIATION;

EID: 0029782934     PISSN: 01433334     EISSN: None     Source Type: Journal    
DOI: 10.1093/carcin/17.8.1549     Document Type: Article

References (17)

1. Kunala, S. and Brash, D.E. (1992) Excision repair at individual bases of the Escherichia coli lacl gene: relation to mutation hot spots and transcription coupling activity. Proc. Natl Acad. Sci. USA, 89, 11031-11035.
2. Kunala, S. and Brash, D.E. (1994) Intragenic domains of strand-specific repair in Escherichia coli. J. Mol. Biol., 246, 264-272.
3. Pfeiffer, G.P., Steigerwald, S.D., Mueller, P.R., Wold, B. and Riggs, A.D. (1989) Genomic sequencing and methylation analysis by ligation mediated PCR. Science, 246, 810-813.
4. Mueller, P.R. and Wold, B. (1989) In vivo footprinting of a muscle specific enhancer by ligation mediated PCR. Science, 246, 780-786.
5. Grimaldi, K.A., McAdam, S.R., Souhami, R.L. and Hartley, J.A. (1994) DNA damage by anti-cancer agents resolved at the nucleotide level of a single copy gene: evidence for a novel binding site for cisplatin in cells. Nucleic Acids Res., 22, 2311-2317.
6. Kalinowsky, D.P., Illenye, S. and Van Houten, B. (1992) Analysis of DNA damage and repair in murine leukemia L1210 cells using a quantitative polymerase chain reaction assay. Nucleic Acids Res., 20, 3485-3494.
7. Maxam, A.M. and Gilbert, W. (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol., 65, 499-560.
8. Ambrose, B.J.B. and Pless, R.C. (1987) DNA sequencing: chemical methods. Methods Enzymol., 152, 522-538.
9. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
10. Carrier, W.L. and Setlow, R.B. (1970) Endonuclease from Micrococcus luteus which has activity toward ultraviolet-irradiated deoxyribonucleic acid: purification and properties. J. Bacteriol., 102, 178-186.
11. Hultman, T., Ståhl, S., Hornes, E. and Uhlen, M. (1989) Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res., 17, 4937-4946.
12. Gordon, L.K. and Haseltine, W.A. (1980) Comparison of the cleavage of pyrimidine dimer by the bacteriophage T4 and Micrococcus luteus UV-specific endonucleases. J. Biol. Chem., 255, 12047-12050.
13. Dodson, M.L., Michaels, M.L. and Lloyd, R.S. (1994) Unified catalytic mechanism for DNA glycosylases. J. Biol. Chem., 269, 32709-32712.
14. O'Connor, T.R. and Laval, J. (1989) Physical association of the 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase of Escherichia coli and an activity nicking DNA at apurinic/apyrimidinic sites. Proc. Natl Acad. Sci. USA, 86, 5222-5226.
15. Bailly, V., Verly, W.G., O'Connor, T.R. and Laval, J. (1989) Mechanism of DNA strand nicking at apurinic/apyrimidinic sites by Escherichia coli [formamidopyrimidine] DNA glycosylase. Biochem. J., 262, 581-589.
16. Hertzberg, R.P. and Dervan, P.B. (1984) Cleavage of DNA with methidiumpropyl-EDTA-iron(II): reaction conditions and products analyses. Biochemistry, 23, 3934-3945.
17. Imlay, J.A. and Linn, S. (1988) DNA damage and oxygen radical toxicity. Science, 240, 1302-1309.