Effects of heat shock and teprenone on ethanol-induced damage to cultured rabbit gastric mucosal cells

Journal of Physiology and Pharmacology

Volumn 47, Issue 3, 1996, Pages 433-441

  Takahashi, S ^a   Yamazaki, T ^a   Okabe, S ^a  

^a KYOTO PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

cytoprotection; ethanol; gastric mucosal cells; heat shock; teprenone

Indexed keywords

ALCOHOL; CYCLOHEXIMIDE; DACTINOMYCIN; HEAT SHOCK PROTEIN 72; PROTECTIVE AGENT; PROTEIN ANTIBODY; TEPRENONE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL PROTECTION; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; HEAT SHOCK; IMMUNOBLOTTING; MALE; MEMBRANE PERMEABILITY; MITOCHONDRION; MUCOSA CELL; NONHUMAN; RABBIT; STOMACH MUCOSA LESION;

ANIMALS; ANTI-ULCER AGENTS; BLOTTING, WESTERN; CELL SURVIVAL; CELLS, CULTURED; CYCLOHEXIMIDE; DACTINOMYCIN; DITERPENES; ETHANOL; GASTRIC MUCOSA; HEAT-SHOCK PROTEINS; HEAT-SHOCK RESPONSE; HSP72 HEAT-SHOCK PROTEINS; RABBITS;

EID: 0029782793     PISSN: 08675910     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Nakamura K, Rokutan K, Marui N, Aoike A, Kawai K. Induction of heat shock proteins and their implication in protection against ethanol-induced damage in cultured guinea pig gastric mucosal cells. Gastroenterology 1991; 101: 161-166.
2. Musch MW, Ciancio MJ, Sarge K, Chang EB. Induction of heat shock protein 70 protects intestinal epithelial IEC-18 cells from oxidant and thermal injury. Am J Physiol 1996; 270: C429-C436.
3. Murakami N, Oketani K, Fujisaki H, Wakabayashi T, Ohgo T. Antiulcer effect of geranylgeranylacetone, a new acyclic polyisoprenoid, on experimentally induced gastric and duodenal ulcers in rats. Arzneim Forsch Drug Research 1981; 31: 799-804.
4. Terano A, Shiga J, Hiraishi H, Ota S, Sugimoto T. Protective action of tetraprenylacetone against ethanol-induced damage in rat gastric mucosa. Digestion 1986; 35: 182-188.
5. Arakawa T, Yamada H, Nakamura A et al. Gastric cytoprotection by tetraprenylacetone in human subjects. Digestion 1988; 39: 111-117.
6. Kunisaki C, Sugiyama M. Effect of teprenone on acute gastric mucosal lesions induced by cold-restraint stress. Digestion 1992; 53: 45-53.
7. Hirakawa T, Yamada M, Teshima S, Nikawa T, Rokutan K. Teprenone exerts its cytoprotective action through induction of heat shock proteins in cultured gastric mucosal cells. Gastroenterology 1995; 108: suppl. A114.
8. Watanabe S, Hirose M, Yasuda T, Miyazaki A, Sato N. Role of actin and calmodulin in migration and proliferation of rabbit gastric mucosal cells in culture. J Gastroent Hepatol 1994; 9: 325-333.
9. Philips HJ. Dye exclusion tests for cell viability. In: Tissue culture methods and applications. PF Krause, MK Patterson (eds), Academic Press, New York, 1973, pp. 406-408.
10. Takahashi S, Nakamura E, Okabe S. Stimulatory effect of leminoprazole on secretion and synthesis of mucus by rabbit gastric mucosal cells. J Pharmacol Exp Ther 1995; 275: 1396-1401.
11. 2 receptor antagonist, roxatidine, stimulates mucus secretion and synthesis by cultured rabbit gastric mucosal cells. J Physiol Pharmacol 1995; 46: 503-511.
12. Takahashi S, Okabe S. Stimulatory effects of sucralfate on secretion and synthesis of mucus by rabbit gastric mucosal cells: Involvement of phospholipase C. Dig Dis Sci 1996; 41: 498-504.
13. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63.
14. Terano A, Ivey KJ, Stachura J et al. Cell culture of rat gastric fundic mucosa. Gastroenterology 1982; 83: 1280-1291.
15. Laemmli UK Cleavage of structural proteins during the assembly of the head of bacteriophage T-4. Nature 1970; 227: 680-685.
16. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350-4354.
17. Levenson W, Oppermann H, Jackson J. Transition series metals and sulfhydryl reagents induce the synthesis of four proteins in eukaryotic cells. Biochim Biophys Acta 1980; 606: 170-180.
18. Lmdquist S. The heat shock response. Ann Rev Biochem 1986; 55: 1151-1191.
19. Polla BS. A role for heat shock proteins in inflammation. Immunol Today 1988; 9: 134-137.
20. Disa S, Manilla AC, Scher CD. Purification and characterization of a platelet derived growth factor and heavy metal-modulated nuclear protein. J Biol Chem 1989; 264: 15993-15999.
21. Cantoni L, ROSSI C, Rizzardini M, Gadina M, Ghezzi P. Interleukin-1 and tumour necrosis factor induce hepatic haem oxygenase. Biochem J 1991; 279: 891-894.
22. Laszlo A, Li GC. Heat-resistant variants of Chinese hamster fibroblasts altered in expression of heat shock protein. Proc Natl. Acad Sci USA 1985; 82: 8029-8033.
23. Johnston RN, Kucey BL. Competitive inhibition of hsp 70 gene expression causes thermosensitivity. Science 1988; 242: 1551-1554.
24. Riabowol KT, Mizzen LA, Welch WJ. Heat shock is lethal to fibroblasts microinjected with antibodies against hsp 70. Science 1988; 242: 433-436.
25. Glavin GB, Szabo S, Johnson BR et al. Isolated rat gastric mucosal cells: Optical conditions for cell harvesting, measures of viability and direct cytoprotection. J Pharmacol Exp Ther 1996; 276: 1174-1179.
26. Terano A, Hiraishi H, Ota S. Sugimoto T. Geranylgeranylacetone, a novel antiulcer drug, stimulates mucus synthesis and secretion in rat gastric mucosal cells. Digestion 1986; 33: 206-210.
27. Takahashi, S. Tsukahara, T. Okabe S. Protective effects of leminoprazole on various agents-induced damage to rabbit gastric mucosal cells. Gastroenterology 1996; 110 (Suppl): A270.