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6
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7
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9344269191
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note
-
k antibody 14-4-4, followed by size exclusion chromatography with Superdex-200 (Pharmacia).
-
-
-
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8
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9344270862
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-
note
-
k-Hsp of data collection quality by equilibrating 5 to 10 mg/ml of purified protein against 16 to 18% polyethylene glycol 4000 (Fluka), 2% ethylene glycol, 200 mM ammonium sulfate, and 100 mM citrate (pH 5.2 to 5.6). Crystals were cryoprotected by the addition of 20% polyethylene glycol 4000, 20% ethylene glycol, and 10% glycerol.
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9
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0026586413
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B. D. Evavold, S. G. Williams, B. L. Hsu, S. Buus, P. M. Allen, J. Immunol. 148, 347 (1992).
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Evavold, B.D.1
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10
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0027448853
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11
-
-
9344258389
-
-
note
-
sym = 0.087 and 92.1% completion between 20 and 2.7 A The solvent contents are 55% and 60% for the respective crystals with two heterodimeric complexes per asymmetric unit.
-
-
-
-
13
-
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0000356656
-
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2-terminal regions (P-8 to P-5 of Hb and P-7 to P-5 of Hsp) or the COOH-terminal 10 residues of either the α or β chains, as these regions appear highly disordered in the electron density maps. The density for residues β107 to β113 was poor in both peptide complexes; this segment is modeled as polyalanine.
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Jones, T.A.1
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14
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0028935078
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Ignatowicz, L.1
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15
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0028348369
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L. J. Stern et al., Nature 368, 215 (1994).
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Stern, L.J.1
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16
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0028823585
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P. Ghosh, M. Amaya, E. Mellins, D. C. Wiley, ibid. 378, 457 (1995).
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Ghosh, P.1
Amaya, M.2
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17
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0027217789
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J. H. Brown et al., ibid. 364, 33 (1993).
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Brown, J.H.1
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21
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0026502380
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J. L. Jorgensen, U. Esser, B. F. de St. Groth, P. A. Reay, M. M. Davis, Nature 355, 224 (1992).
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Jorgensen, J.L.1
Esser, U.2
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Davis, M.M.5
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22
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0026434554
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B. D. Evavold and P. M. Allen, Science 252, 1308 (1991); B. D. Evavold, J. Sloan-Lancaster, B. L. Hsu, P. M. Allen, J. Immunol. 150, 3131 (1993); L. M. Spain, J. L. Jorgensen, M. M. Davis, L. J. Berg, ibid. 152, 1709 (1994).
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Evavold, B.D.1
Allen, P.M.2
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23
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0027174663
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B. D. Evavold and P. M. Allen, Science 252, 1308 (1991); B. D. Evavold, J. Sloan-Lancaster, B. L. Hsu, P. M. Allen, J. Immunol. 150, 3131 (1993); L. M. Spain, J. L. Jorgensen, M. M. Davis, L. J. Berg, ibid. 152, 1709 (1994).
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Evavold, B.D.1
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Hsu, B.L.3
Allen, P.M.4
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24
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0028281677
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B. D. Evavold and P. M. Allen, Science 252, 1308 (1991); B. D. Evavold, J. Sloan-Lancaster, B. L. Hsu, P. M. Allen, J. Immunol. 150, 3131 (1993); L. M. Spain, J. L. Jorgensen, M. M. Davis, L. J. Berg, ibid. 152, 1709 (1994).
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Spain, L.M.1
Jorgensen, J.L.2
Davis, M.M.3
Berg, L.J.4
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26
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0025313181
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P. E. Jensen, J. Exp. Med. 171, 1779 (1990); A. Sette et al., J. Immunol. 148, 844 (1992); J. Boniface et al., Biochemistry 32, 11761 (1993); P. Reay, D. Wettstein, M. M. Davis, EMBO J. 11, 2829 (1992).
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Jensen, P.E.1
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27
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0026546814
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P. E. Jensen, J. Exp. Med. 171, 1779 (1990); A. Sette et al., J. Immunol. 148, 844 (1992); J. Boniface et al., Biochemistry 32, 11761 (1993); P. Reay, D. Wettstein, M. M. Davis, EMBO J. 11, 2829 (1992).
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Sette, A.1
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28
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0027361113
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P. E. Jensen, J. Exp. Med. 171, 1779 (1990); A. Sette et al., J. Immunol. 148, 844 (1992); J. Boniface et al., Biochemistry 32, 11761 (1993); P. Reay, D. Wettstein, M. M. Davis, EMBO J. 11, 2829 (1992).
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Boniface, J.1
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29
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0026643116
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P. E. Jensen, J. Exp. Med. 171, 1779 (1990); A. Sette et al., J. Immunol. 148, 844 (1992); J. Boniface et al., Biochemistry 32, 11761 (1993); P. Reay, D. Wettstein, M. M. Davis, EMBO J. 11, 2829 (1992).
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Reay, P.1
Wettstein, D.2
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-
32
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9344222023
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M. L. Connolly, ibid. 3, 19 (1985). Solvent-accessible surface calculations were performed with a 1.4 Å probe sphere.
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(1985)
J. Mol. Graphics
, vol.3
, pp. 19
-
-
Connolly, M.L.1
-
33
-
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9344228809
-
-
note
-
kHb crystal. Perhaps dimer packing in the different space groups, slight differences in pH, or small changes in the dimer interface controlled the sequestering of the sulfate ion. There is no obvious influence of the peptide or linker on sulfate coordination.
-
-
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-
34
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9344242156
-
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note
-
We thank J. Clements, F. Crawford, H. Kozono, D. Parker, and J. White for help in making the soluble class II molecules and H.-E. Aronson, K. Choi, C. Lima, X. Zhao, and X. Zhu for aid in the synchrotron data collection and Nir Ben-Tal for electrostatic discussions. Coordinates have been deposited in the Brookhaven Protein Data Bank (entries 1IEA and 1IEB) and are available via e-mail (fremont@ columbia.edu).
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