Evaluation of cyclohexenoesculetin-β-D-galactoside and 8- hydroxyquinoline-β-D-galactoside as substrates for the detection of β- galactosidase

Applied and Environmental Microbiology

Volumn 62, Issue 10, 1996, Pages 3868-3870

  James, A L ^a   Perry, J D ^b   Ford, M ^b   Armstrong, L ^a   Gould, F K ^b  

^a NORTHUMBRIA UNIVERSITY   (United Kingdom)

^b FREEMAN HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

8 QUINOLINOL DERIVATIVE; BETA GALACTOSIDASE; ESCULIN; GALACTOSIDE;

ARTICLE; ENTEROBACTERIACEAE; ENZYME ASSAY; ENZYME SUBSTRATE; NONHUMAN; PERFORMANCE; SYNTHESIS;

ENTEROBACTERIACEAE;

EID: 0029781936     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.10.3868-3870.1996     Document Type: Article

References (9)

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2. Berg, J. D., and L. Fiksdal. 1988. Rapid detection of total and fecal coliforms in water by enzymatic hydrolysis of 4-methylumbelliferone-β-D-galactoside. Appl. Environ. Microbiol. 54:2118-2122.
3. Dalet, F., and T. Segovia. 1995. Evaluation of a new agar in Uricult-Trio for rapid detection of Escherichia coli in urine. J. Clin. Microbiol. 33:1395-1398.
4. James, A. L., and P. Yeoman. 1987. Detection of specific bacterial enzymes by high contrast metal chelate formation. I. 8-Hydroxyquinoline β-D-glucoside, an alternative to aesculin in the differentiation of members of the family Enterobacteriaceae. Zentralbl. Bakteriol. Mikrobiol. Hyg. Ser. A 267:188-193.
5. James, A. L., and P. Yeoman. 1988. Detection of specific bacterial enzymes by high contrast metal chelate formation. II. Specific detection of Escherichia coli on multipoint inoculated plates using 8-hydroxyquinoline β-D-glucuronide. Zentralbl. Bakteriol. Mikrobiol. Hyg. Ser. A 267:316-321.
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7. Lowe, G. H. 1962. The rapid detection of lactose fermentation in paracolon organisms by the demonstration of β-D-galactosidase. J. Med. Lab. Technol. 19:21.
8. Manafi, M., W. Kneifel, and S. Bascomb. 1991. Fluorogenic and chromogenic substrates used in bacterial diagnostics. Microbiol. Rev. 55:335-348.
9. 12-FDG as substrates for β-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl. Environ. Microbiol. 60:4638-4641.