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LFS sometimes caused an increase in amplitude of the fiber volley, resulting in an apparent reduction in magnitude of LTD. When the increase was more than 5%, data were excluded from analysis.
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The absence of paired-pulse facilitation between the pathways was used as the criterion to determine that the stimulated pathways were independent.
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The effectiveness of APV was tested in the CA1 region. In the presence of 25 μM APV prepared from the same stock solution used in this study, tetanic stimulation of Schaffer collaterals (at 100 Hz for 1 s, twice) did not induce STP or LTP of field EPSPs (n = 4).
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We thank Y. Hayashi for his comments on the manuscript. This work was supported by a Ministry of Education, Science and Culture (Japan) grant-in-aid (to T.M. and T.T.), the Uehara Memorial Foundation (T.M. and T.T.), and the Brain Science Foundation (T.M.).
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