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Volumn 273, Issue 5275, 1996, Pages 648-650

Presynaptic long-term depression at the hippocampal mossy fiber-CA3 synapse

Author keywords

[No Author keywords available]

Indexed keywords

GLUTAMATE RECEPTOR; METABOTROPIC RECEPTOR; N METHYL DEXTRO ASPARTIC ACID RECEPTOR;

EID: 0029780982     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5275.648     Document Type: Review
Times cited : (142)

References (42)
  • 3
    • 0026458086 scopus 로고
    • S. M. Dudek and M. F. Bear, Proc. Natl. Acad. U.S.A. 89, 4363 (1992); R. M. Mulkey and R. C. Malenka, Neuron 9, 967 (1992).
    • (1992) Neuron , vol.9 , pp. 967
    • Mulkey, R.M.1    Malenka, R.C.2
  • 4
    • 13344261412 scopus 로고    scopus 로고
    • T. Kutsuwada et al., Neuron 16, 333 (1996).
    • (1996) Neuron , vol.16 , pp. 333
    • Kutsuwada, T.1
  • 11
    • 0024761050 scopus 로고
    • S. Williams and D. Johnston, ibid. 3, 583 (1989); D. Jaffe and D. Johnston, J. Neurophysiol. 64, 948 (1990).
    • (1989) Neuron , vol.3 , pp. 583
    • Williams, S.1    Johnston, D.2
  • 17
    • 0028053818 scopus 로고
    • T. Battistin and E. Cherubini, Eur. J. Neurosci. 6, 1750 (1994). They reported that homosynaptic LTD could be induced by tetanic stimulation at mossy fiber synapses only in young rats (<2 weeks old). However, it is likely that their EPSPs originated from inputs other than mossy fibers, because in their study the recording was made in the stratum radiatum, through which mossy fibers do not pass.
    • (1994) Eur. J. Neurosci. , vol.6 , pp. 1750
    • Battistin, T.1    Cherubini, E.2
  • 19
    • 0028271580 scopus 로고
    • A. Kirkwood, S. M. Dudek, J. T. Gold, C. D. Aizenman, M. F. Bear, Science 260, 1518 (1993); A. Kirkwood and M. F. Bear, J. Neurosci. 14, 3404 (1994).
    • (1994) J. Neurosci. , vol.14 , pp. 3404
    • Kirkwood, A.1    Bear, M.F.2
  • 20
    • 0029558545 scopus 로고
    • Transverse hippocampal slices (400 μm thick) were prepared from adult mice (>6 weeks old) (Charles River: ICR mouse) after mice were decapitated under halothane anesthesia. Slices were maintained in a holding chamber for at least 1 hour and were then transferred to a recording chamber and submerged beneath a continuously superfusing solution. Mossy fiber field EPSPs were recorded in the stratum lucidum with a glass microelectrode filled with 2 or 3 M NaCI (pipette resistance, 1 to 5 megohm) as described previously [Y. Takei et al., J. Cell Biol. 131, 1789 (1995)]. At the end of each experiment, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 20 μM) was added to the bath to assess a fiber volley component (see Fig. 1A), which was subtracted from the EPSP on analysis. All data were expressed as means ± SEM, CNQX, APV, MCPG, ACPD, and kynurenic acid were purchased from Tocris Cookson (Bristol, U.K.), Nicardipine (Sigma) was dissolved in dimethyl sulfoxide (DMSO) and diluted to the superfusing solution (final concentration of DMSO was 0.02% v/v). An Axopatch 1D amplifier was used to record field EPSPs. Records were filtered at 1 kHz, digitized at 10 kHz, and collected on a computer (466/LV, Dell). All experiments were carried out at room temperature (24° to 28°C).
    • (1995) J. Cell Biol. , vol.131 , pp. 1789
    • Takei, Y.1
  • 21
    • 9344259204 scopus 로고    scopus 로고
    • note
    • It is quite unlikely that mossy fiber LTD is contaminated with the depression of nonmossy fiber EPSPs because LFS induced only very small LTD (<5%) at associational-commissural synapses (n = 3).
  • 22
    • 9344230946 scopus 로고    scopus 로고
    • note
    • LFS sometimes caused an increase in amplitude of the fiber volley, resulting in an apparent reduction in magnitude of LTD. When the increase was more than 5%, data were excluded from analysis.
  • 23
    • 0024464603 scopus 로고
    • J. E. Bradler and G. Barrionuevo, Synapse 4, 132 (1989); M. G. Weisskopf, R. A. Zalutsky, R. A. Nicoll, Nature 362, 423 (1993).
    • (1989) Synapse , vol.4 , pp. 132
    • Bradler, J.E.1    Barrionuevo, G.2
  • 25
    • 9344226848 scopus 로고    scopus 로고
    • note
    • The absence of paired-pulse facilitation between the pathways was used as the criterion to determine that the stimulated pathways were independent.
  • 27
    • 9344263535 scopus 로고    scopus 로고
    • note
    • The effectiveness of APV was tested in the CA1 region. In the presence of 25 μM APV prepared from the same stock solution used in this study, tetanic stimulation of Schaffer collaterals (at 100 Hz for 1 s, twice) did not induce STP or LTP of field EPSPs (n = 4).
  • 32
    • 9344244195 scopus 로고    scopus 로고
    • note
    • On a few occasions, a clear enhancement of EPSPs was observed during MCPG application. Because this enhancement occurred for unknown reasons and was not observed consistently, these data were excluded from analysis. Fiber volleys were not altered by MCPG when it was applied either alone or during LFS.
  • 33
    • 9344246387 scopus 로고    scopus 로고
    • note
    • In four out of six slices, MCPG blocked LTD almost completely (less than 5% depression). In some experiments, LFS was applied twice, initially in the presence of MCPG and then after MCPG was washed out. The first LFS caused almost no depression (2.9 ± 1.0%) but the second induced robust depression (16.0 ± 3.6%) (n = 3).
  • 37
    • 0027410488 scopus 로고
    • Y. Tanabe, M. Masu, T. Ishii, R. Shigemoto, S, Nakanishi, Neuron 8, 169 (1992); Y. Tanabe et al., J. Neurosci. 13, 1372 (1993).
    • (1993) J. Neurosci. , vol.13 , pp. 1372
    • Tanabe, Y.1
  • 40
    • 9344251199 scopus 로고    scopus 로고
    • note
    • 2+ chelator BAPTA (10 mM) through the whole-cell patch pipette (22.5 ± 11.3% depression 30 min after LFS; n = 3).
  • 41
    • 9344255435 scopus 로고    scopus 로고
    • M. Yokoi et al., Science 273, 645 (1996).
    • (1996) Science , vol.273 , pp. 645
    • Yokoi, M.1
  • 42
    • 9344221154 scopus 로고    scopus 로고
    • note
    • We thank Y. Hayashi for his comments on the manuscript. This work was supported by a Ministry of Education, Science and Culture (Japan) grant-in-aid (to T.M. and T.T.), the Uehara Memorial Foundation (T.M. and T.T.), and the Brain Science Foundation (T.M.).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.