The gene for γ-glutamylcysteine synthetase from Thiobacillus ferrooxidans has low homology to its Escherichia coli equivalent and is linked to the gene for citrate synthase

Microbiology

Volumn 142, Issue 9, 1996, Pages 2543-2548

  Powles, Rosamund ^a   Deane, Shelly ^a   Rawlings, Douglas ^a  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Citrate synthase; Molecular cloning; Thiobacillus serraoxidans; glutamylcysteine synthetase

Indexed keywords

CITRATE SYNTHASE; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE;

ACIDITHIOBACILLUS FERROOXIDANS; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GROWTH; BACTERIUM MUTANT; CONTROLLED STUDY; COSMID; ESCHERICHIA COLI; GENE ISOLATION; GENE SEQUENCE; GENETIC LINKAGE; GENETIC TRANSCRIPTION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ACIDITHIOBACILLUS FERROOXIDANS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; THIOBACILLUS;

EID: 0029780426     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-142-9-2543     Document Type: Article

References (20)

1. Aiba, H., Adhaya, S. & de Crombrugge, B. (1981). Evidence for two functional gal promoters in intact E. coli cells. J Biol Chem 256, 11905-11910.
2. Clennel, A.-M., Johnston, B. & Rawlings, D. E. (1995). Structure and function of Tn5467, a Tn21-like transposon located on the Thiobacillus ferrooxidans broad-host-range plasmid pTF-FC2. Appl Environ Microbiol 61, 4223-4229.
3. Dennda, G. & Kula, M.-R. (1986). Purification and evaluation of the glutathione-synthesizing enzymes of Candida boidinii for cell-free synthesis of glutathione. J Biotechnol 4, 143-158.
4. Devereux, J., Haeberli, P. & Smithies, O. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12, 387-395.
5. Hell, R. & Bergmann, L. (1990). γ-Glutamylcysteine synthetase in higher plants: catalytic properties and subcellular localization. Planta 180, 603-612.
6. Hissin, P. J. & Hilf, R. (1976). A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem 74, 214.
7. Huang, C.-S., Moore, W. R. & Meister, A. (1988). On the active site thiol of γ-glutamylcysteine synthetase: relationships to catalysis, inhibition, and regulation. Proc Natl Acad Sci USA 85, 2464-2468.
8. Huang, C. S., Anderson, M. E. & Meister, A. (1993). Amino acid sequence and function of the light subunit of rat kidney gamma-glutamylcysteine synthetase. J Biol Chem 268, 20578-20583.
9. Kumagai, H., Nakayama, R. & Tochikura, T. (1982). γ-Glutamylcysteine synthetase from Proteus mirabilis. Agric Biol Chem 46, 1301-1309.
10. Lim, C.-J., Gleason, F. K. & Fuchs, J. A. (1986). Cloning, expression and characterization of the Anabaena thioredoxin gene is Escherichia coli. J Bacteriol 168, 1258-1264.
11. Lindström, E. B., Gunneriusson, E. & Tuovinen, O. H. (1992). Bacterial oxidation of refractory sulfide ores for gold recovery. Crit Rev Biotechnol 12, 133-155.
12. May, M. J. & Leaver, C. J. (1994). Arabidopsis thaliana γ-glutamylcysteine synthetase is structurally unrelated to mammalian, yeast, and Escherichia coli homologs. Proc Natl Acad Sci USA 91, 10059-10063.
13. Meister, A. & Anderson, M. E. (1983). Glutathione. Annu Rev Biochem 52, 711-760.
14. Powles, R. E., Deane, S. M. & Rawlings, D. E. (1995). Molecular and genetic analysis of a thioredoxin gene from Thiobacillus ferrooxidans. Microbiology 141, 2175-2181.
15. Ramesar, R. S. (1988). Developmental genetic studies on Thiobacillus ferrooxidans. PhD thesis, University of Cape Town, South Africa.
16. Richman, P. G. & Meister, A. (1975). Regulation of γ-glutamylcysteine synthetase by nonallosteric feedback inhibition by glutathione. J Biol Chem 250, 1422-1426.
17. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
18. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74, 3650-3654.
19. Seelig, G. F., Simondsen, R. P. & Meister, A. (1984). Reversible dissociation of γ-glutamylcysteine synthetase into two subunits. J Biol Chem 259, 9345-9347.
20. Wood, D. O., Williamson, L. R., Winkler, H. H. & Krause, D. C. (1987). Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene. J Bacteriol 169, 3564-3572.