CLEAR: A novel family of highly cross-linked polymeric supports for solid-phase peptide synthesis

Journal of the American Chemical Society

Volumn 118, Issue 30, 1996, Pages 7083-7093

  Kempe, Maria ^a,b   Barany, George ^a  

^a University of Minnesota   (United States)

^b LUND UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

GASTRIN; LEUCINE ENKEPHALINAMIDE;

ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PEPTIDE SYNTHESIS; TECHNIQUE;

EID: 0029778868     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja954196s     Document Type: Article

References (56)

1. For preliminary accounts of portions of this work, see: (a) Kempe, M.; Barany, G. In Peptides: Chemistry, Structure & Biology, Proceedings of the Fourteenth American Peptide Symposium; Kaumaya, P. T. P., Hodges, R. S., Eds.; Mayflower Scientific Ltd.: Kingswinford, England, 1996; pp 865-866. (b) Kempe, M.; Barany, G. In Innovations and Perspectives in Solid Phase Synthesis and Combinatorial Chemical Libraries, 1995; Epton. R., Ed.; Mayflower Worldwide Ltd.: Kingswinford, England, 1996; in press.
2. For preliminary accounts of portions of this work, see: (a) Kempe, M.; Barany, G. In Peptides: Chemistry, Structure & Biology, Proceedings of the Fourteenth American Peptide Symposium; Kaumaya, P. T. P., Hodges, R. S., Eds.; Mayflower Scientific Ltd.: Kingswinford, England, 1996; pp 865-866. (b) Kempe, M.; Barany, G. In Innovations and Perspectives in Solid Phase Synthesis and Combinatorial Chemical Libraries, 1995; Epton. R., Ed.; Mayflower Worldwide Ltd.: Kingswinford, England, 1996; in press.
3. Abbreviations used for amino acids and the designation of peptides follow the rules of the IUPAC-IUB Commission of Biochemical Nomenclature in J. Biol. Chem. 1972, 247, 977-983. The following additional abbreviations are used: AAA, amino acid analysis, AIBN, 2,2′-azobisisobutyronitrile; BOP, benzotriazolyl N-oxytris(dirnethylamino)phosphonium hexafluorophosphate; CLEAR, title supports of this paper, DIPCDI, N.N′-diisopropylcarbodiimide; DMF, N.N-dimethylformamide; EDT, 1,2-ethanedithiol, Fmoc, 9-fluorenylmethyloxycarbonyl; HPLC, high-performance liquid chromatography; HOAt, 1-hydroxy-7-azabenzotriazole; HOBt, 1-hydroxybenzotriazole; IRAA, internal reference amino acid; NMM, N-methyl morpholine; PAL, 5-(4-aminomethyl-3,5-dimethoxyphenoxy)-valeric acid; PEG, polyethylene glycol; PS, polystyrene; SEM, scanning electron microscopy; SPPS, solid-phase peptide synthesis; TFA. trifluoroacetic acid. All solvent ratios are volume/volume. All amino acids used were of the L-configuration.
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49. 4OH for 2 weeks shows such a pattern [for details, see Experimental section; mass spectra are in supporting information: Figures 17-19]. FT-MS ESI performed under the same conditions on crude ACP(65-74) amide did not show this pattern of signals separated by 44 amu but only the expected signals due to the peptides [for spectra see supporting information: Figure 20].
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