Measurement of radiation-induced DNA double-strand breaks in human diploid fibroblasts from keloid and normal skin by single-cell gel electrophoresis

Plastic and Reconstructive Surgery

Volumn 98, Issue 5, 1996, Pages 821-826

  Ma, Shiuh ^a   Chang, Wushou P ^b   Fang, Rong Hwang ^b  

^a TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA;

ARTICLE; CONTROLLED STUDY; DIPLOIDY; DNA STRAND BREAKAGE; FLUORESCENCE MICROSCOPY; GEL ELECTROPHORESIS; HUMAN; HUMAN CELL; HUMAN TISSUE; KELOID; PRIORITY JOURNAL; RADIATION INJURY; RADIOSENSITIVITY; SKIN FIBROBLAST;

EID: 0029778828     PISSN: 00321052     EISSN: None     Source Type: Journal    
DOI: 10.1097/00006534-199610000-00011     Document Type: Article

References (24)

1. Ketchum, L. D., Cohen, I. K., and Masters, F. W. Hypertrophic scars and keloids: A collective review. Plast. Reconstr. Surg. 53: 140, 1974.
2. Rockwell, W. B., Cohen, I. K., and Ehrlich, H. P. Keloids and hypertrophic scars: A comprehensive review. Plast. Reconstr. Surg. 84: 827, 1989.
3. Muir, I. F. K. On the nature of keloid and hypertrophic scars. Br. J. Plast. Surg. 3: 61, 1990.
4. Borok, T. L., Bray, M., Sinclair, I., et al. Role of ionizing irradiation for 393 keloids. Int. J. Radiat. Oncol. Biol. Phys. 15: 865, 1988.
5. Cohen, I. K., and McCoy, B. J. The biology and control of surface overheating. World J. Surg. 4: 289, 1980.
6. Datubo-Brown, D. D. Keloids: A review of the literature. Br. J. Plast. Surg. 43: 70, 1990.
7. Kovalic, J. J., and Perez, C. A. Radiation therapy following keloidectomy: A 20-year experience. Int. J. Radiat. Oncol. Biol. Phys. 17: 77, 1989.
8. Lawrence, W. T. In search of the optimal treatment of keloids: Report of a series and a review of the literature. Ann. Plast. Surg. 27: 164, 1991.
9. Diegelmann, R. F., Cohen, I. K., and McCoy, B. J. Growth kinetics and collagen synthesis of normal skin, normal scar and keloid fibroblasts in vitro. J. Cell. Physiol. 98: 341, 1979.
10. Abergel, R. P., Pizzurro, D., and Meeker C. A. Biochemical composition of the connective tissue in keloids and analysis of collagen metabolism in keloid fibroblast cultures. J. Invest. Dermatol. 84: 384, 1985.
11. Ala-Kokko, L., Rintala, A., and Savolainen, E.-R. Collagen gene expression in keloids: Analysis of collagen metabolism and type I, III, IV, and V procollagen mRNAs in keloid tissue and keloid fibroblast cultures. J. Invest. Dermatol. 89: 238, 1987.
12. Russell, S. B., Trupin, K. M., Rodriguez-Eaton, S., et al. Reduced growth-factor requirement of keloid-derived fibroblasts may account for tumor growth. Proc. Natl. Acad. Sci. U.S.A. 85: 587, 1988.
13. Russell, S. B., Trupin. J. S., Myers J. C., et al. Differential glucocorticoid regulation of collagen mRNAs in human dermal fibroblasts. J. Biol. Chem. 264: 13,730, 1989.
14. Peltonen, J., Hsiao, L. L., Jaakkola, S., et al. Activation of collagen gene expression in keloids: Colocalization of type I and VI collagen and transforming growth factor-b1 mRNA. J. Invest. Dermatol. 97: 240, 1991.
15. Ma, S., Fang, R. H., and Chang, W. P. Heterogeneity in radiosensitivity of human diploid fibroblasts from keloids and normal skin. Cell Biol. Int. (in press).
16. Iliakis, G. The role of DNA double-strand breaks in ionizing radiation-induced killing of eukaryotic cells. Bioessays 13: 641, 1991.
17. Ostling, O., and Johanson, K. J. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 123: 291, 1984.
18. Singh, N. P., McCoy, M. T., Tice, R. R., et al. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175: 184, 1988.
19. Olive, P. L., Banath, J. P., and Durand, R. E. Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay. Radiat. Res. 122: 86, 1990.
20. Muller, W.-U., Bauch, T., Streffer, C., et al. Comet assay studies of radiation-induced DNA damage and repair in various tumour cell lines. Int. J. Radiat. Biol. 65: 315, 1994.
21. Chiu, J. H., Kao, H. L., Wu, L. H., Chang, H. M., et al. Prediction of relapse or survival after resection in human hepatomas by DNA flow cytometry. J. Clin. Invest. 89: 539, 1992.
22. Olive, P. L., Wlodek, D., Durand, R. E., et al. Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell Res. 198: 259, 1992.
23. Schor, S. L., Schor, A. M., Grey, A. M., et al. Foetal and cancer patient fibroblasts produce an autocrine migration stimulating factor not made by normal adult cells. J. Cell Sci. 90: 391, 1988.
24. Schor, S. L., Schor, A., M., and Rushton, G. Fibroblasts from cancer patients display a mixture of both foetal and adult-like phenotype characteristics. J. Cell Sci. 90: 401, 1988.