Single-tube chemical cleavage of mismatch: Successive treatment with hydroxylamine and osmium tetroxide

BioTechniques

Volumn 21, Issue 2, 1996, Pages 216-220

  Ramus, Susan J ^a   Cotton, Richard G H ^a  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

HETERODUPLEX; HYDROXYLAMINE; OSMIUM TETRAOXIDE;

ARTICLE; BASE MISPAIRING; CHEMICAL ANALYSIS; DNA CLEAVAGE; DNA DETERMINATION; DNA STRAND; GENE MUTATION;

EID: 0029778293     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96212bm10     Document Type: Article

References (5)

1. Cotton, R.G.H. 1993. Current methods of mutation detection. Mutat. Res. 285:125-144.
2. Cotton, R.G.H., H.H.M. Dahl, S. Forrest, D. Howells, S.J. Ramus, R.E. Bishop, I. Dianzani, J.A. Saleeba, E. Polombo, M.J. Anderson, C.M. Milner and R.D. Campbell. 1993. Analysis of sequence contexts flanking T.G mismatches leads to predictions about reactivity of the mismatched T to osmium tetroxide. DNA Cell Biol. 12:945-949.
3. Cotton, R.G.H., N.R. Rodrigues and R.D. Campbell. 1988. Reactivity of cytosine and thymine in single-base pair mismatches with hydroxylamine and osmium tetroxide and its application to the study of mutations. Proc. Natl. Acad. Sci. USA 85:4397-4401.
4. Dobi, A.L., K. Matsumoto, E. Santha and D.V. Agoston. 1994. Guanine specific chemical sequencing of DNA by osmium tetroxide. Nucleic Acids Res. 22:4846-4847.
5. Ramus, S.J. and R.G.H. Cotton. 1995. CCM: Chemical Cleavage of Mismatch, p. 50-54. Laboratory Protocols for Mutation Detection. Oxford University Press, Oxford.