Streamlined protocol for polytene chromosome in situ hybridization

BioTechniques

Volumn 21, Issue 2, 1996, Pages 226-230

  Blackman, Ronald K ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CHROMOSOME ANALYSIS; DATA BASE; DNA PROBE; DROSOPHILA; FLUORESCENCE IN SITU HYBRIDIZATION; IN SITU HYBRIDIZATION; POLYTENE CHROMOSOME;

EID: 0029776375     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96212bm12     Document Type: Article

References (11)

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2. Engels, W.R., C.R. Preston, P. Thompson and W.B. Eggleston. 1986. in situ hybridization to Drosophila salivary chromosomes with biotinylated DNA probes and alkaline phosphatase. Focus 8:6-8.
3. The FlyBase Consortium. 1996. FlyBase: the Drosophila database. Nucleic Acids Res. 24:53-56.
4. Hepperle, B. 1995. A simple device for preparing well flattened Drosophila polytene chromosomes for in situ hybridization. Dros. Info. Serv. 76:175-176.
5. Kim, W. and M.G. Kidwell. 1994. In situ hybridization to polytene: chromosomes using digoxigenin-11-dUTP-labelled probes. Dros. Info. Serv. 75:44-46.
6. Lim, J.K. 1993. In situ hybridization with biotinylated DNA. Dros. Info. Serv. 72:73-76.
7. Pardue, M.L. 1986. In situ hybridization to DNA of chromosomes and nuclei, p. 111-137. In D.B. Roberts (Ed.), Drosophila: A Practical Approach. IRL Press, Oxford.
8. Pardue, M.L. 1994. Looking at polytene chromosomes. Methods Cell Biol. 44:333-351.
9. Phillips, A.M., J. Martin and D.G. Bedo. 1994. In situ hybridization to polytene chromosomes of Drosophila melanogaster and other dipterans, p. 193-209. In K.H.A. Choo (Ed.), In Situ Hybridization Protocols. Humana Press, Totowa.
10. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
11. Saunders, R.D.C. 1994. In situ hybridization to Drosophila polytene chromosomes, p. 183-191. In K.H.A. Choo (Ed.), In Situ Hybridization Protocols. Humana Press, Totowa.