Isotype-directed enrichment of B cells by magnetic beads in the generation of immunoreactive human monoclonal antibodies

Hybridoma

Volumn 15, Issue 4, 1996, Pages 255-262

  McKnight, Michael E ^a   Mukerjee, Sonjoy ^a   Nasoff, Marc ^a   Gaskins, Chris ^a   Glassy, Mark C ^a  

^a Novopharm Biotech Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN G4; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY SC GM 4; STREPTAVIDIN; UNCLASSIFIED DRUG;

ANTIGEN RECOGNITION; ARTICLE; B LYMPHOCYTE; CANCER PATIENT; CELL SEPARATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; HYBRIDOMA; MELANOMA CELL; MOLECULAR WEIGHT; NEUROBLASTOMA CELL; PANCREAS TUMOR; PRIORITY JOURNAL; TUMOR CELL LINE;

EID: 0029775812     PISSN: 0272457X     EISSN: None     Source Type: Journal    
DOI: 10.1089/hyb.1996.15.255     Document Type: Article

References (23)

1. Kemmner W, Moldenhauer G, Schlag P, and Brossmer R: Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads. J Immunol Methods 1992;147:197.
2. A Lea T, Vartdal F, Nustad K, Funderud S, Berge A, Elkinsen T, Schmid R, Stenstad P, and Ugelstad J: Monosized, magnetic polymer particles: Their use in separation of cells and subcellular components, and in the study of lymphocyte function in vitro. J Mol Recognition 1988;1:9.
3. Sharma P, Lonneborg A, and Stougaard P: PCR-based construction of subtractive cDNA library using magnetic beads. Biotechniques 1993;15:610.
4. Lundkvist A, Horling J, Athlin L, Rosen A, and Niklasson B: Neutralizing human monoclonal antibodies against Puumala virus, causative agent of nephropathia epidemica: A novel method using antigen-coated magnetic beads for specific B cell isolation. J Gen Virol 1993;74:1303.
5. Ossendorp F, Bruning P, Van den Brink J, and DeBoer M: Efficient selection of high-affinity B cell hybridomas using antigen-coated magnetic beads. J Immunol Methods 1989;120:191.
6. Casali P, Inghirami G, Nakamura M, Davies T, and Notkins A: Human monoclonals from antigen-specific selection of B lymphocytes and transformation by EBV. Science 1986;234:476.
7. Glaser R, Volk H-D, Liebenthal C, Jahn S, and Grunow R: Immortalization of magnatically separated human lymphocytes by electrofusion. Hum Antibod Hybridoma 1990;1:111.
8. Glassy M, and McKnight M: Pharming the human lymph node. Exp Opin Invest Drugs 1994;3:1057.
9. Glassy M, and McKnight M: A novel drug discovery programme utilizing the human immune response. Curr Opin Invest Drugs 1993;2:853.
10. McKnight M, and Glassy M: The autoimmunity of cancer as applied to drug development. Exp Opin Invest Drugs 1995;4:629.
11. Glassy M: Generation of UC 729-6 and SHFP-1 derived human hybridomas and their growth in serum-free media. J Tissue Cult Methods 1989;12:85.
12. Glassy M: Immortalization of human lymphocytes from a tumor-involved lymph node. Cancer Res 1987;47:5181.
13. Koda K, Glassy M, and Chang H: Generation of human monoclonal antibodies against colon cancer. Arch Surg 1990;125:1591.
14. Glassy M, Handley H, Cleveland P, and Royston I: An enzyme immunofiltration assay useful for detecting human monoclonal antibody. J Immunol Methods 1983;58:119.
15. Glassy M, and Surh C: Immunodetection of cell bound antigens using both mouse and human monoclonal antibodies. J Immunol Methods 1985;81:115.
16. Glassy M, and Cleveland P: Use of mouse and human monoclonal antibodies in enzyme immunofiltration. Methods Enzymol 1986;121:525.
17. Gaffar S, Royston I, and Glassy M: Strategies for the design and use of tumor reactive human monoclonal antibodies. BioEssays 1986;4:119.
18. Glassy M: Production methods for generating human monoclonal antibodies. Hum Antibod Hybridomas 1993;4:154.
19. Chang H, Koda K, McKnight M, and Glassy M: Tumor-associated antigens recognized by human monoclonal antibodies. Ann Surg Oncol 1994;1:213.
20. Dean P: Data analysis in cell kinetics research. In: Techniques in Cell Cycle Research. Gray J, and Darzynkiewicz Z (Eds.). Humana Press, Clifton, NJ, 1987, pp. 207-253.
21. Guesdon J, Ternyck T, and Avrameas S: The use of avidin-biotin interactions in immunoenzymatic techniques. J Histochem Cytochem 1979;27:1131.
22. Viale G, Flamini G, Grassi F, Buffa R, Natali P, Pelagi M, Leoni F, Menard S, and Siccardi A: Idiotypic replica of an anti-human tumor associated antigen monoclonal antibody: Analysis on monoclonal Abl of Ab3 fine specificity. J Immunol 1989;143:4338.
23. Perelmutter L: Cell surface IgG subclasses. Monographs Allergy 1986;19:36.