Technical Note / Evaluation of an Automated Pyrogallol Red-Molybdate Method for the Measurement of Urinary Protein in Rats

Clinical Chemistry and Laboratory Medicine

Volumn 34, Issue 7, 1996, Pages 569-572

  Lynch, Karen M ^a   Sellers, Teresa S ^a   Gossett, Kent A ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MOLYBDIC ACID; PYROGALLOL;

ANIMAL EXPERIMENT; ARTICLE; AUTOANALYSIS; CONTROLLED STUDY; FEMALE; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN URINE LEVEL; RAT; URINALYSIS;

ANIMALS; COLORING AGENTS; FEMALE; MALE; MOLYBDENUM; PROTEINURIA; PYROGALLOL; RATS; RATS, SPRAGUE-DAWLEY; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; ROSANILINE DYES;

ANIMALIA;

EID: 0029775048     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/cclm.1996.34.7.569     Document Type: Article

References (11)

1. McElderry L. A., Tarbit I. F., Cassells-Smith A. J., Six methods for urinary protein compared. Clin Chem 1982; 28:356-360.
2. Dilena B. A., Penberty L. A., Fraser C. G., Six methods for determining urinary protein compared. Clin Chem 1983; 29:553-557.
3. Orsonneau J-L, Douet P., Massoubre C., Lustenburger P., Bernard S. An improved pyrogallol red-molybdate method for determining total urinary protein. Clin Chem 1989; 357 11:2233-2236.
4. Macart M., Gerbaut L. Evaluation of an improved Coomassie dye binding method for urinary protein assay. Clin Chim Acta 1984: 141:77-84.
5. Nishi H. H., Kestner J., Elin R. J., Four methods for determining total protein compared by using purified protein fractions from human serum. Clin Chem 1985; 31:95-98.
6. Kluwe W. M., Rapid, automated measurements of urinary protein and glucose concentrations. J Pharmacol Meth 1981; 5:235-240.
7. Evans G. O., Parsons C. E., Potential errors in the measurement of total protein in male rat urine using test strips. Lab Anim 1981; 2:27-31.
8. Fujita Y., Mori I., Kitano S. Color reaction between pyrogallol red-molybdenum (VI) complex and protein. Bunseki Kagaku 1983; 32:379-386.
9. Watanabe N., Kamei S., Ohkubo A., Yamanaka M., Ohsawa S., Makno K., et al, Urinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 Automated Analyzer. Clin Chem 1986; 32:1551-1554.
10. Westgard J. O., de Vos D. J., Hunt M. R., Quam E. F., Carey R. N., Garber C. C., Concepts and practices in the evaluation of laboratory methods. II. Experimental procedures. Am J Med Technol 1978; 44:420-430.
11. Goren M. P., Li JTL. The Coomassie brilliant blue method underestimates drug-induced tubular proteinuria. Clin Chem 1986; 32:386-388.