Amino acid substitutions in the rat Na+, K+-ATPase α2-subunit alter the cation regulation of pump current expressed in HeLa cells

Journal of Physiology

Volumn 495, Issue 3, 1996, Pages 733-742

  Yamamoto, S ^a   Kuntzweiler, T A ^a   Wallick, E T ^a   Sperelakis, N ^a   Yatani, A ^a  

^a UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); AMINO ACID; COMPLEMENTARY DNA; GLUTAMINE; LEUCINE; MUTANT PROTEIN; OUABAIN; POTASSIUM ION; SODIUM ION;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STRUCTURE; ENZYME SUBUNIT; HELA CELL; HUMAN; HUMAN CELL; PATCH CLAMP; POTASSIUM CURRENT; PRIORITY JOURNAL; PROTEIN EXPRESSION; SODIUM CURRENT; STRUCTURE ACTIVITY RELATION;

EID: 0029774272     PISSN: 00223751     EISSN: None     Source Type: Journal    
DOI: 10.1113/jphysiol.1996.sp021629     Document Type: Article

References (28)

1. + pump is voltage insensitive. Proceedings of the National Academy of Sciences of the USA 85, 3412-3416.
2. + concentration in internally perfused cardiac cells. Biochimica et Biophysics Acta 1065, 269-271.
3. CANESSA, C. M., HORISBERGER, J. D., LOUVARD, D. & ROSSIER, B. C. (1992). Mutation of a cysteine in the first transmembrane segment of Na,K-ATPase α subunit confers ouabain resistance. EMBO Journal 11, 1681-1687.
4. +-ATPase by specific and nonspecific proteases with preservation of cation occlusion sites. Journal of Biochemical Chemistry 267, 1150-1158.
5. CHEN, C. & OKAYAMA, H. (1987). High-efficiency transformation of mammalian cells by plasmid DNA. Molecular and Cellular Biology 7, 2745-2752.
6. COHEN, I. S., DATYNER, N. B., GINTANT, G. A., MULRINE, N. K. & PENNEFATHER, P. (1987). Properties of an electrogenic sodium-potassium pump in isolated canine Purkinje myocytes. Journal of Physiology 383, 251-267.
7. 86Rb release from an occluded state of the Na,K-pump reflects the rate of dephosphorylation or dearsenylation. Journal of Biological Chemistry 263, 7961-7969.
8. GADSBY, D. C. & NAKAO, M. (1989). Steady-state current-voltage relationship of the Na/K pump in guinea pig ventricular myocytes. Journal of General Physiology 94, 511-537.
9. GLYNN, J. M. & KARLISH, S. J. D. (1990). Occluded cations in active transport. Annual Review of Biochemistry 59, 171-205.
10. HEYSE, S., WCDDEL, I., APELL, H-J. & STÜRMER, W. (1994). Partial reactions of the Na,K-ATPase: Determination of rate constants. Journal of General Physiology 104, 197-240.
11. JEWELL, E. A. & LINGREL, J. B. (1991). Comparison of the substrate dependence properties of the rat Na,K-ATPase α1, α2, and α3 isoforms expressed in HeLa cells. Journal of Biological Chemistry 266, 16925-16930.
12. JEWELL-MOTZ, E. A. & LINGREL, J. B. (1993). Site-directed mutagenesis of the Na,K-ATPase: Consequences of substitutions of negatively-charged amino acids in the transmembrane domains. Biochemistry 32, 13523-13530.
13. JøRGENSEN, P. L. (1992). Na,K-ATPase, structure and transport mechanism. In Molecular Aspects of Transport Proteins, ed. DEPONT, J. J., pp. 1-26. Elsevier Science Publishers, Holland,
14. o activation of the Na-K pump in adult rat cardiac myocytes. American Journal of Physiology 266, C37-41.
15. +-induced conformational change. Journal of Biological Chemistry 270, 2993-3000.
16. LÄUGER, P. (1991). Na,K-ATPase. In Electrogenic Ion Pumps, ed. LÄUGER, P., pp. 168-225. Sinauer Associates, Inc., Sunderland, MA, USA.
17. +-ATPase. Journal of Biological Chemistry 269, 19659-19662.
18. LINGREL, J. B., ORLOWSKI, J., SHULL, M. M. & PRICE, E. M. (1990). Molecular genetics of Na,K-ATPase. Progress in Nucleic Acid Research and Molecular Biology 38, 37-89.
19. NAKAUAWA, T., SHIRASAKI, T., WAKAMORI, M., FUKUDA, A. & AKAIKE, N. (1990). Excitatory amino acid response in isolated nucleus tractus solitarii neurons of the rat. Neuroscience Research 8, 114-123.
20. NAKAO, M. & GADSBY, D. C. (1989). [Na] and [K] dependence of the Na/K pump current-voltage relationship in guinea pig ventricular myocytes. Journal of General Physiology 94, 539-565.
21. PEDEMONTE, C. H. & KAPLAN, J. H. (1990). Chemical modification as an approach to elucidation of sodium pump structure-function relations. American Journal of Physiology 258, C1-23.
22. RAKOWSKI, R. F., GADSBY, D. C. & DE WEER, P. (1989). Stoichiometry and voltage dependence of the sodium pump in voltage-clamped, internally dialyzed squid giant axon. Journal of General Physiology 93, 903-941.
23. +-ATPase. Biochimica et Biophysica Acta 1154, 201-222.
24. VILSEN, B. (1993). A Glu329-Gln variant of the α-subunit of the rat kidney Na,K-ATPase can sustain transport of Na and K and Na,K-activated ATP hydrolysis with normal turnover numbers. FEBS Letters 333, 44-55.
25. +-ATPase α1 subunit. American Journal of Physiology 270, C457-464.
26. +. Biophysical Journal 68, A256.
27. + channels. American Journal of Physiology 258, H1507-1514.
28. + channel currents by quinidine. Circulation Research 73, 351-359.