Purification and activities of the Rhodobacter capsulatus RpoN (σ(N)) protein

Molecular Microbiology

Volumn 21, Issue 2, 1996, Pages 233-245

  Cannon, Wendy ^a   Missailidis, Sotiris ^b   Austin, Sara ^a,d   Moore, Madeleine ^b   Drake, Alex ^c   Buck, Martin ^a,e  

^a UNIVERSITY OF SUSSEX   (United Kingdom)

^b UNIVERSITY OF YORK   (United Kingdom)

^c UNIVERSITY OF LONDON   (United Kingdom)

^d JOHN INNES CENTRE   (United Kingdom)

^e IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN; RNA POLYMERASE;

ARTICLE; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DETERMINATION; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN PURIFICATION; RHODOBACTER CAPSULATUS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE; RHODOBACTER CAPSULATUS; RHODOPSEUDOMONAS;

EID: 0029773417     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.6181334.x     Document Type: Article

References (26)

1. Austin, S., Henderson, N., and Dixon, R. (1987) Requirements for transcriptional activation in vitro of the nitrogen-regulated glnA and nifLA promoters from Klebsiella pneumoniae: dependence on activator concentration. Mol Microbiol 1: 92-100.
2. Buck, M., and Cannon, W. (1992) Specific binding of the transcription factor sigma-54 to promoter DNA. Nature 358: 422-424.
3. Buck, M., and Cannon, W. (1994) A simple procedure for visualising protein-nucleic acid complexes by photochemical crosslinking. Nucl Acids Res 22: 1119-1120.
4. Cannon, W., and Buck, M. (1992a) Central domain of the positive central protein NifA and its role in transcriptional activation. J Mol Biol 225: 271-286.
5. Cannon, W., and Buck, M. (1992b) Activator-independent formation of a closed complex between sigma-54 holoenzyme and nifH and nifU promoters of Klebsiella pneumoniae. Mol Microbiol 6: 1625-1630.
6. 54 to promoter DNA. Mol Microbiol 8: 287-298.
7. N). Mol Microbiol 11: 227-236.
8. 54) protein and promoter DNA in closed promoter complexes. Nucl Acids Res 23: 351-356.
9. N: bipartite functions. J Mol Biol 249: 781-803.
10. Cullen, P.J., Foster-Hartnett, D., Gabbert, K.K., and Kranz, R.G. (1994) Structure and expression of the alternative sigma factor, RpoN, in Rhodobacter capsulatus: physiological relevance of an autoactivatable nfU2-rpoN super-operon. Mol Microbiol 11: 51-65.
11. Gross, C.A., Lonetto, M., and Losick, R. (1992) Sigma Factors. In Transcriptional Regulation. Yamamoto, K., and McKnight, S. (eds). Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
12. 54. J Biol Chem 269: 373-378.
13. Jones, R., and Haselkorn, R. (1989) The DNA sequence of the Rhodobacter capsulatus ntrA, ntrB and ntrC gene analogues required for nitrogen fixation. Mol Gen Genet 215: 507-516.
14. Karls, R.K., Jin, D.J., and Donohue, T.J. (1993) Transcription properties of RNA polymerase holoenzymes isolated from the purple non-sulphur bacterium Rhodobacter sphaeroides. J Bacteriol 175: 7629-7638.
15. 54 (ntrA)-dependent genes is probably united by a common mechanism. Microbiol Rev 53: 367-376.
16. Meijer, W., and Tabita, F. (1992) Isolation and characterisation of the nifUSUW-rpoN gene cluster from Rhodobacter sphaeroides. J Bacteriol 174: 3855-3866.
17. N). Mol Microbiol 10: 903-909.
18. 54 is involved in recognition of the -13 promoter element. J Bacteriol 174: 7221-7226.
19. N) holoenzyme closed promoter complexes. J Biol Chem 269: 11563-11571.
20. Preker, P., and Klipp, W. (1992) Mapping and characterisation of the promoter elements of the regulatory nif genes rpoN, nifA1, and nifA2 in Rhodobacter capsulatus. Mol Microbiol 6: 1035-1047.
21. Sander, C., and Schneider, R. (1991) Database of homology-derived protein structures. Proteins 9: 56-68.
22. 54. Cell 62: 945-954.
23. Studier, F.W., and Moffat, B.A. (1986) Use of bacteriophage T7-RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189: 113-130.
24. 54. Proc Natl Acad Sci USA 91: 2120-2124.
25. 54 in setting the rate and temperature dependence of promoter melting in vivo. J Biol Chem 267: 24762-24768.
26. Wong, C., Tintut, Y., and Gralla, J.D. (1994) The domain structure of Sigma 54 as determined by analysis of a set of deletion mutants. J Mol Biol 236: 81-90.